Determination of whole genome sequence of human cytomegalovirus circulating in Japan and discovery of geographic genome structure in UL148 gene
Human cytomegalovirus (HCMV), a member of the Herpesviridae family, is prevalent worldwide. HCMV is generally asymptomatic but causes severe disease in immunocompromised patients or infants who are congenitally infected. Recent advances in sequencing technology have led to the rapid expansion of the...
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Elsevier
2025-03-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170225000164 |
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| author | Yuji Wada Ken Ishioka Tatsuo Suzutani |
| author_facet | Yuji Wada Ken Ishioka Tatsuo Suzutani |
| author_sort | Yuji Wada |
| collection | DOAJ |
| description | Human cytomegalovirus (HCMV), a member of the Herpesviridae family, is prevalent worldwide. HCMV is generally asymptomatic but causes severe disease in immunocompromised patients or infants who are congenitally infected. Recent advances in sequencing technology have led to the rapid expansion of the HCMV genetic database, providing a comprehensive resource for studying viral genetics. Although genetic investigations have been vigorously performed in European countries, information on the whole-genome sequence of HCMV in the East Asian region remains limited. In this study, we determined whole-genome sequences of two clinical isolates of HCMV circulating in Japan. Partial genome sequences of 26 genes in UL/b' region were also identified using additional seven clinical isolates. Phylogenetic analysis of the UL148 gene revealed a characteristic genetic clade predominantly constructed from HCMV isolates from Japan and China, suggesting a geographic gene structure in the East Asian region. We consider that this research will contribute to expanding the genetic database of HCMV and unveiling novel genetic characteristics of HCMV in Asia. |
| format | Article |
| id | doaj-art-ba577d1753964095a598478ef5d2f59f |
| institution | Kabale University |
| issn | 1872-7492 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj-art-ba577d1753964095a598478ef5d2f59f2025-02-05T04:31:05ZengElsevierVirus Research1872-74922025-03-01353199540Determination of whole genome sequence of human cytomegalovirus circulating in Japan and discovery of geographic genome structure in UL148 geneYuji Wada0Ken Ishioka1Tatsuo Suzutani2Corresponding author.; Department of Microbiology, School of Medicine, Fukushima Medical University, JapanDepartment of Microbiology, School of Medicine, Fukushima Medical University, JapanDepartment of Microbiology, School of Medicine, Fukushima Medical University, JapanHuman cytomegalovirus (HCMV), a member of the Herpesviridae family, is prevalent worldwide. HCMV is generally asymptomatic but causes severe disease in immunocompromised patients or infants who are congenitally infected. Recent advances in sequencing technology have led to the rapid expansion of the HCMV genetic database, providing a comprehensive resource for studying viral genetics. Although genetic investigations have been vigorously performed in European countries, information on the whole-genome sequence of HCMV in the East Asian region remains limited. In this study, we determined whole-genome sequences of two clinical isolates of HCMV circulating in Japan. Partial genome sequences of 26 genes in UL/b' region were also identified using additional seven clinical isolates. Phylogenetic analysis of the UL148 gene revealed a characteristic genetic clade predominantly constructed from HCMV isolates from Japan and China, suggesting a geographic gene structure in the East Asian region. We consider that this research will contribute to expanding the genetic database of HCMV and unveiling novel genetic characteristics of HCMV in Asia.http://www.sciencedirect.com/science/article/pii/S0168170225000164Human cytomegalovirusPhylogenetic analysis |
| spellingShingle | Yuji Wada Ken Ishioka Tatsuo Suzutani Determination of whole genome sequence of human cytomegalovirus circulating in Japan and discovery of geographic genome structure in UL148 gene Virus Research Human cytomegalovirus Phylogenetic analysis |
| title | Determination of whole genome sequence of human cytomegalovirus circulating in Japan and discovery of geographic genome structure in UL148 gene |
| title_full | Determination of whole genome sequence of human cytomegalovirus circulating in Japan and discovery of geographic genome structure in UL148 gene |
| title_fullStr | Determination of whole genome sequence of human cytomegalovirus circulating in Japan and discovery of geographic genome structure in UL148 gene |
| title_full_unstemmed | Determination of whole genome sequence of human cytomegalovirus circulating in Japan and discovery of geographic genome structure in UL148 gene |
| title_short | Determination of whole genome sequence of human cytomegalovirus circulating in Japan and discovery of geographic genome structure in UL148 gene |
| title_sort | determination of whole genome sequence of human cytomegalovirus circulating in japan and discovery of geographic genome structure in ul148 gene |
| topic | Human cytomegalovirus Phylogenetic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0168170225000164 |
| work_keys_str_mv | AT yujiwada determinationofwholegenomesequenceofhumancytomegaloviruscirculatinginjapananddiscoveryofgeographicgenomestructureinul148gene AT kenishioka determinationofwholegenomesequenceofhumancytomegaloviruscirculatinginjapananddiscoveryofgeographicgenomestructureinul148gene AT tatsuosuzutani determinationofwholegenomesequenceofhumancytomegaloviruscirculatinginjapananddiscoveryofgeographicgenomestructureinul148gene |