Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress
Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects. This study identified a unique isoform of 18S rRNA gene (864 bp) from a novel wild mushroom (SMK-1) (GenBank accession number: SUB3267363). Thin layer chromatographic (TLC) profiling of the methanolic...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2021-07-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000549 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557191805009920 |
|---|---|
| author | M. Subbulakshmi Sugapriya Dhanasekaran S. Abirami M. Kannan R. Palaniappan Divya Venugopal |
| author_facet | M. Subbulakshmi Sugapriya Dhanasekaran S. Abirami M. Kannan R. Palaniappan Divya Venugopal |
| author_sort | M. Subbulakshmi |
| collection | DOAJ |
| description | Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects. This study identified a unique isoform of 18S rRNA gene (864 bp) from a novel wild mushroom (SMK-1) (GenBank accession number: SUB3267363). Thin layer chromatographic (TLC) profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and flavonoid fractions with retention factor (Rf) values of 0.955 and 0.927 respectively. The GC/MS chromatograms of the SMK-1 methanolic extract identified the main bioactive compound was phenol, 5-methyl-2-(1-methylethyl) (74.00%) (thymol). The radical scavenging activity for the flavonoid fraction was greater than the phenolic fractions (Rf–phenolics fractions > Rf–flavonoid fractions) with the antioxidant activity more than that of standard ascorbic acid. Also, the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5 μg/mL to 80 μg/mL in a dose-dependent manner. This present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefits. The application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug. |
| format | Article |
| id | doaj-art-ba530aac1f1645aba693648a8ea8b98a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-ba530aac1f1645aba693648a8ea8b98a2025-02-03T05:39:08ZengTsinghua University PressFood Science and Human Wellness2213-45302021-07-01104452459Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stressM. Subbulakshmi0Sugapriya Dhanasekaran1S. Abirami2M. Kannan3R. Palaniappan4Divya Venugopal5Department of Microbiology, K. R. College of Arts and Science, Kovilpatti, Tamil Nadu, IndiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Wadi Al Dawaser, Saudi Arabia; Corresponding author.Department of Microbiology, Kamaraj College, Thoothukudi, Tamil Nadu, IndiaResearch Department of Zoology, V.H.N. Senthikumara Nadar College (Autonomus) Virudhunagar, Tamil Nadu, IndiaResearch Department of Computer Science, V.H.N. Senthikumara Nadar College (Autonomus) Virudhunagar, Tamil Nadu, IndiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Wadi Al Dawaser, Saudi ArabiaMushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects. This study identified a unique isoform of 18S rRNA gene (864 bp) from a novel wild mushroom (SMK-1) (GenBank accession number: SUB3267363). Thin layer chromatographic (TLC) profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and flavonoid fractions with retention factor (Rf) values of 0.955 and 0.927 respectively. The GC/MS chromatograms of the SMK-1 methanolic extract identified the main bioactive compound was phenol, 5-methyl-2-(1-methylethyl) (74.00%) (thymol). The radical scavenging activity for the flavonoid fraction was greater than the phenolic fractions (Rf–phenolics fractions > Rf–flavonoid fractions) with the antioxidant activity more than that of standard ascorbic acid. Also, the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5 μg/mL to 80 μg/mL in a dose-dependent manner. This present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefits. The application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug.http://www.sciencedirect.com/science/article/pii/S2213453021000549AntioxidantAnti-proliferationDietary mushroomsHeLa cellsNovel MushroomsThymol. |
| spellingShingle | M. Subbulakshmi Sugapriya Dhanasekaran S. Abirami M. Kannan R. Palaniappan Divya Venugopal Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress Food Science and Human Wellness Antioxidant Anti-proliferation Dietary mushrooms HeLa cells Novel Mushrooms Thymol. |
| title | Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress |
| title_full | Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress |
| title_fullStr | Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress |
| title_full_unstemmed | Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress |
| title_short | Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress |
| title_sort | phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress |
| topic | Antioxidant Anti-proliferation Dietary mushrooms HeLa cells Novel Mushrooms Thymol. |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000549 |
| work_keys_str_mv | AT msubbulakshmi phylogeneticanalysisandprotectiveeffectsofthymolanditschromatographicfractionsfromanovelwildmushroomincombatingoxidativestress AT sugapriyadhanasekaran phylogeneticanalysisandprotectiveeffectsofthymolanditschromatographicfractionsfromanovelwildmushroomincombatingoxidativestress AT sabirami phylogeneticanalysisandprotectiveeffectsofthymolanditschromatographicfractionsfromanovelwildmushroomincombatingoxidativestress AT mkannan phylogeneticanalysisandprotectiveeffectsofthymolanditschromatographicfractionsfromanovelwildmushroomincombatingoxidativestress AT rpalaniappan phylogeneticanalysisandprotectiveeffectsofthymolanditschromatographicfractionsfromanovelwildmushroomincombatingoxidativestress AT divyavenugopal phylogeneticanalysisandprotectiveeffectsofthymolanditschromatographicfractionsfromanovelwildmushroomincombatingoxidativestress |