Homocysteine enhances the excitability of cultured hippocampal neurons without altering the gene expression of voltage-gated ion channels
Abstract Elevated plasma homocysteine (Hcy) levels lead to hyperhomocysteinemia, a condition associated with various neurological disorders affecting multiple brain regions, including the hippocampus. In this study, we investigated the effects of exposing cultured rat hippocampal neurons to Hcy conc...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-04-01
|
| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13041-025-01205-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850145549550878720 |
|---|---|
| author | Alzbeta Filipova Matus Tomko Katarina Ondacova Lucia Dubiel-Hoppanova Nikola Chmúrčiaková Leoš Cmarko Robin N. Stringer Norbert Weiss Lubica Lacinova |
| author_facet | Alzbeta Filipova Matus Tomko Katarina Ondacova Lucia Dubiel-Hoppanova Nikola Chmúrčiaková Leoš Cmarko Robin N. Stringer Norbert Weiss Lubica Lacinova |
| author_sort | Alzbeta Filipova |
| collection | DOAJ |
| description | Abstract Elevated plasma homocysteine (Hcy) levels lead to hyperhomocysteinemia, a condition associated with various neurological disorders affecting multiple brain regions, including the hippocampus. In this study, we investigated the effects of exposing cultured rat hippocampal neurons to Hcy concentrations corresponding to mild, moderate, and severe hyperhomocysteinemia. A short 24-hour exposure had minimal effects, whereas prolonged exposure up to 14 days moderately enhanced hippocampal excitability without altering the gene expression of voltage-dependent calcium, sodium, or potassium channels or intracellular calcium levels. These findings suggest that Hcy-induced changes in neuronal excitability may contribute to neuropathologies associated with hyperhomocysteinemia. |
| format | Article |
| id | doaj-art-ba4801d6f4384a1c84cfa9fd73d7c49c |
| institution | OA Journals |
| issn | 1756-6606 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj-art-ba4801d6f4384a1c84cfa9fd73d7c49c2025-08-20T02:28:04ZengBMCMolecular Brain1756-66062025-04-011811410.1186/s13041-025-01205-xHomocysteine enhances the excitability of cultured hippocampal neurons without altering the gene expression of voltage-gated ion channelsAlzbeta Filipova0Matus Tomko1Katarina Ondacova2Lucia Dubiel-Hoppanova3Nikola Chmúrčiaková4Leoš Cmarko5Robin N. Stringer6Norbert Weiss7Lubica Lacinova8Center of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of SciencesCenter of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of SciencesCenter of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of SciencesCenter of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of SciencesInstitute of Biology and Medical Genetics, First Faculty of Medicine, Charles UniversityInstitute of Biology and Medical Genetics, First Faculty of Medicine, Charles UniversityDepartment of Pathophysiology, Third Faculty of Medicine, Charles UniversityCenter of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of SciencesCenter of Biosciences, Institute of Molecular Physiology and Genetics, Slovak Academy of SciencesAbstract Elevated plasma homocysteine (Hcy) levels lead to hyperhomocysteinemia, a condition associated with various neurological disorders affecting multiple brain regions, including the hippocampus. In this study, we investigated the effects of exposing cultured rat hippocampal neurons to Hcy concentrations corresponding to mild, moderate, and severe hyperhomocysteinemia. A short 24-hour exposure had minimal effects, whereas prolonged exposure up to 14 days moderately enhanced hippocampal excitability without altering the gene expression of voltage-dependent calcium, sodium, or potassium channels or intracellular calcium levels. These findings suggest that Hcy-induced changes in neuronal excitability may contribute to neuropathologies associated with hyperhomocysteinemia.https://doi.org/10.1186/s13041-025-01205-xHyperhomocysteinemiaHippocampal excitabilityTranscriptomicsVoltage gated ion channelsIntracellular calcium |
| spellingShingle | Alzbeta Filipova Matus Tomko Katarina Ondacova Lucia Dubiel-Hoppanova Nikola Chmúrčiaková Leoš Cmarko Robin N. Stringer Norbert Weiss Lubica Lacinova Homocysteine enhances the excitability of cultured hippocampal neurons without altering the gene expression of voltage-gated ion channels Molecular Brain Hyperhomocysteinemia Hippocampal excitability Transcriptomics Voltage gated ion channels Intracellular calcium |
| title | Homocysteine enhances the excitability of cultured hippocampal neurons without altering the gene expression of voltage-gated ion channels |
| title_full | Homocysteine enhances the excitability of cultured hippocampal neurons without altering the gene expression of voltage-gated ion channels |
| title_fullStr | Homocysteine enhances the excitability of cultured hippocampal neurons without altering the gene expression of voltage-gated ion channels |
| title_full_unstemmed | Homocysteine enhances the excitability of cultured hippocampal neurons without altering the gene expression of voltage-gated ion channels |
| title_short | Homocysteine enhances the excitability of cultured hippocampal neurons without altering the gene expression of voltage-gated ion channels |
| title_sort | homocysteine enhances the excitability of cultured hippocampal neurons without altering the gene expression of voltage gated ion channels |
| topic | Hyperhomocysteinemia Hippocampal excitability Transcriptomics Voltage gated ion channels Intracellular calcium |
| url | https://doi.org/10.1186/s13041-025-01205-x |
| work_keys_str_mv | AT alzbetafilipova homocysteineenhancestheexcitabilityofculturedhippocampalneuronswithoutalteringthegeneexpressionofvoltagegatedionchannels AT matustomko homocysteineenhancestheexcitabilityofculturedhippocampalneuronswithoutalteringthegeneexpressionofvoltagegatedionchannels AT katarinaondacova homocysteineenhancestheexcitabilityofculturedhippocampalneuronswithoutalteringthegeneexpressionofvoltagegatedionchannels AT luciadubielhoppanova homocysteineenhancestheexcitabilityofculturedhippocampalneuronswithoutalteringthegeneexpressionofvoltagegatedionchannels AT nikolachmurciakova homocysteineenhancestheexcitabilityofculturedhippocampalneuronswithoutalteringthegeneexpressionofvoltagegatedionchannels AT leoscmarko homocysteineenhancestheexcitabilityofculturedhippocampalneuronswithoutalteringthegeneexpressionofvoltagegatedionchannels AT robinnstringer homocysteineenhancestheexcitabilityofculturedhippocampalneuronswithoutalteringthegeneexpressionofvoltagegatedionchannels AT norbertweiss homocysteineenhancestheexcitabilityofculturedhippocampalneuronswithoutalteringthegeneexpressionofvoltagegatedionchannels AT lubicalacinova homocysteineenhancestheexcitabilityofculturedhippocampalneuronswithoutalteringthegeneexpressionofvoltagegatedionchannels |