Emerging biotechnologies for engineering liver organoids
The engineering construction of the liver has attracted enormous attention. Organoids, as emerging miniature three-dimensional cultivation units, hold significant potential in the biomimetic simulation of liver structure and function. Despite notable successes, organoids still face limitations such...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24004869 |
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| author | Junqi Zhao Yue Zhi Haozhen Ren Jinglin Wang Yuanjin Zhao |
| author_facet | Junqi Zhao Yue Zhi Haozhen Ren Jinglin Wang Yuanjin Zhao |
| author_sort | Junqi Zhao |
| collection | DOAJ |
| description | The engineering construction of the liver has attracted enormous attention. Organoids, as emerging miniature three-dimensional cultivation units, hold significant potential in the biomimetic simulation of liver structure and function. Despite notable successes, organoids still face limitations such as high variability and low maturity. To overcome these challenges, engineering strategies have been established to maintain organoid stability and enhance their efficacy, laying the groundwork for the development of advanced liver organoids. The present review comprehensively summarizes the construction of engineered liver organoids and their prospective applications in biomedicine. Initially, we briefly present the latest research progress on matrix materials that maintain the three-dimensional morphology of organoids. Next, we discuss the manipulative role of engineering technologies in organoid assembly. Additionally, we outline the impact of gene-level regulation on organoid growth and development. Further, we introduce the applications of liver organoids in disease modeling, drug screening and regenerative medicine. Lastly, we overview the current obstacles and forward-looking perspectives on the future of engineered liver organoids. We anticipate that ongoing innovations in engineered liver organoids will lead to significant advancements in medical applications. |
| format | Article |
| id | doaj-art-ba2f049c43474e85b47c69165aaf9502 |
| institution | Kabale University |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-ba2f049c43474e85b47c69165aaf95022025-01-26T05:04:19ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-03-0145118Emerging biotechnologies for engineering liver organoidsJunqi Zhao0Yue Zhi1Haozhen Ren2Jinglin Wang3Yuanjin Zhao4Department of Hepatobiliary Surgery, Hepatobiliary Institute, Nanjing Drum Tower Hospital, Medical School, Nanjing University, Nanjing, 210008, China; Department of Gastrointestinal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325035, ChinaDepartment of Hepatobiliary Surgery, Hepatobiliary Institute, Nanjing Drum Tower Hospital, Medical School, Nanjing University, Nanjing, 210008, ChinaDepartment of Hepatobiliary Surgery, Hepatobiliary Institute, Nanjing Drum Tower Hospital, Medical School, Nanjing University, Nanjing, 210008, China; Corresponding author.Department of Hepatobiliary Surgery, Hepatobiliary Institute, Nanjing Drum Tower Hospital, Medical School, Nanjing University, Nanjing, 210008, China; Corresponding author.Department of Hepatobiliary Surgery, Hepatobiliary Institute, Nanjing Drum Tower Hospital, Medical School, Nanjing University, Nanjing, 210008, China; Department of Gastrointestinal Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325035, China; Shenzhen Research Institute, Southeast University, Shenzhen, 518038, China; Corresponding author.The engineering construction of the liver has attracted enormous attention. Organoids, as emerging miniature three-dimensional cultivation units, hold significant potential in the biomimetic simulation of liver structure and function. Despite notable successes, organoids still face limitations such as high variability and low maturity. To overcome these challenges, engineering strategies have been established to maintain organoid stability and enhance their efficacy, laying the groundwork for the development of advanced liver organoids. The present review comprehensively summarizes the construction of engineered liver organoids and their prospective applications in biomedicine. Initially, we briefly present the latest research progress on matrix materials that maintain the three-dimensional morphology of organoids. Next, we discuss the manipulative role of engineering technologies in organoid assembly. Additionally, we outline the impact of gene-level regulation on organoid growth and development. Further, we introduce the applications of liver organoids in disease modeling, drug screening and regenerative medicine. Lastly, we overview the current obstacles and forward-looking perspectives on the future of engineered liver organoids. We anticipate that ongoing innovations in engineered liver organoids will lead to significant advancements in medical applications.http://www.sciencedirect.com/science/article/pii/S2452199X24004869Liver organoidBiotechnologyHydrogelMicrofluidicsGenetic engineering |
| spellingShingle | Junqi Zhao Yue Zhi Haozhen Ren Jinglin Wang Yuanjin Zhao Emerging biotechnologies for engineering liver organoids Bioactive Materials Liver organoid Biotechnology Hydrogel Microfluidics Genetic engineering |
| title | Emerging biotechnologies for engineering liver organoids |
| title_full | Emerging biotechnologies for engineering liver organoids |
| title_fullStr | Emerging biotechnologies for engineering liver organoids |
| title_full_unstemmed | Emerging biotechnologies for engineering liver organoids |
| title_short | Emerging biotechnologies for engineering liver organoids |
| title_sort | emerging biotechnologies for engineering liver organoids |
| topic | Liver organoid Biotechnology Hydrogel Microfluidics Genetic engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24004869 |
| work_keys_str_mv | AT junqizhao emergingbiotechnologiesforengineeringliverorganoids AT yuezhi emergingbiotechnologiesforengineeringliverorganoids AT haozhenren emergingbiotechnologiesforengineeringliverorganoids AT jinglinwang emergingbiotechnologiesforengineeringliverorganoids AT yuanjinzhao emergingbiotechnologiesforengineeringliverorganoids |