Effect of ethyl acetate aroma on viability of human breast cancer and normal kidney epithelial cells in vitro
Background: Aromatherapy is used in clinical settings for patients suffering from several chronic and critical diseases such as cancer. Ethyl acetate (EA) is a colorless liquid with a characteristic fruity smell and is naturally present in fruits and wines. Methods: In the present study, the effect...
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| Language: | English |
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Elsevier
2017-03-01
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| Series: | Integrative Medicine Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213422016301780 |
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| author | Mohsin A. Khan Rumana Ahmad Anand N. Srivastava |
| author_facet | Mohsin A. Khan Rumana Ahmad Anand N. Srivastava |
| author_sort | Mohsin A. Khan |
| collection | DOAJ |
| description | Background: Aromatherapy is used in clinical settings for patients suffering from several chronic and critical diseases such as cancer. Ethyl acetate (EA) is a colorless liquid with a characteristic fruity smell and is naturally present in fruits and wines.
Methods: In the present study, the effect of the aroma of EA was evaluated on human breast cancer cell line MDA-MB-231 and normal cell line, Vero. Cell line viability and mechanism of EA cytotoxicity were determined by Trypan blue dye exclusion assay and phase contrast microscopy.
Results: It was found that EA at a concentration of 0.026 M was effective in causing considerable cytotoxicity in breast cancer cells (without even coming in contact with the culture medium and cells), while showing no effect on normal cells. Mechanism of action of EA on cancer and Vero cells was investigated by DNA fragmentation and dye binding assays using agarose gel electrophoresis (AGE) and fluorescence microscopy/cytometry, respectively. It was found that EA aroma induced predominantly necrosis in the cancer cells exposed to it.
Conclusion: A study such as this has not been attempted before and results need further investigation before EA aroma can be used as a complementary therapy. |
| format | Article |
| id | doaj-art-ba2d5e7574ca4934be978b24cbf3ec9b |
| institution | OA Journals |
| issn | 2213-4220 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Integrative Medicine Research |
| spelling | doaj-art-ba2d5e7574ca4934be978b24cbf3ec9b2025-08-20T02:19:26ZengElsevierIntegrative Medicine Research2213-42202017-03-0161475910.1016/j.imr.2016.11.004Effect of ethyl acetate aroma on viability of human breast cancer and normal kidney epithelial cells in vitroMohsin A. Khan0Rumana Ahmad1Anand N. Srivastava2Chairman Research, Research Unit, Era's Lucknow Medical College & Hospital, Lucknow, IndiaDepartment of Biochemistry, Era's Lucknow Medical College and Hospital, Lucknow, IndiaDepartment of Pathology, Era's Lucknow Medical College and Hospital, Lucknow, IndiaBackground: Aromatherapy is used in clinical settings for patients suffering from several chronic and critical diseases such as cancer. Ethyl acetate (EA) is a colorless liquid with a characteristic fruity smell and is naturally present in fruits and wines. Methods: In the present study, the effect of the aroma of EA was evaluated on human breast cancer cell line MDA-MB-231 and normal cell line, Vero. Cell line viability and mechanism of EA cytotoxicity were determined by Trypan blue dye exclusion assay and phase contrast microscopy. Results: It was found that EA at a concentration of 0.026 M was effective in causing considerable cytotoxicity in breast cancer cells (without even coming in contact with the culture medium and cells), while showing no effect on normal cells. Mechanism of action of EA on cancer and Vero cells was investigated by DNA fragmentation and dye binding assays using agarose gel electrophoresis (AGE) and fluorescence microscopy/cytometry, respectively. It was found that EA aroma induced predominantly necrosis in the cancer cells exposed to it. Conclusion: A study such as this has not been attempted before and results need further investigation before EA aroma can be used as a complementary therapy.http://www.sciencedirect.com/science/article/pii/S2213422016301780aromatherapybreast cancercytotoxicityethyl acetateMDA-MB-231 cells |
| spellingShingle | Mohsin A. Khan Rumana Ahmad Anand N. Srivastava Effect of ethyl acetate aroma on viability of human breast cancer and normal kidney epithelial cells in vitro Integrative Medicine Research aromatherapy breast cancer cytotoxicity ethyl acetate MDA-MB-231 cells |
| title | Effect of ethyl acetate aroma on viability of human breast cancer and normal kidney epithelial cells in vitro |
| title_full | Effect of ethyl acetate aroma on viability of human breast cancer and normal kidney epithelial cells in vitro |
| title_fullStr | Effect of ethyl acetate aroma on viability of human breast cancer and normal kidney epithelial cells in vitro |
| title_full_unstemmed | Effect of ethyl acetate aroma on viability of human breast cancer and normal kidney epithelial cells in vitro |
| title_short | Effect of ethyl acetate aroma on viability of human breast cancer and normal kidney epithelial cells in vitro |
| title_sort | effect of ethyl acetate aroma on viability of human breast cancer and normal kidney epithelial cells in vitro |
| topic | aromatherapy breast cancer cytotoxicity ethyl acetate MDA-MB-231 cells |
| url | http://www.sciencedirect.com/science/article/pii/S2213422016301780 |
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