Variability in Chromosome 1 of Select Moroccan Pyrenophora teres f. teres Isolates Overcomes a Highly Effective Barley Chromosome 6H Source of Resistance
Barley net form net blotch (NFNB) is a destructive foliar disease caused by Pyrenophora teres f. teres. Barley line CIho5791, which harbors the broadly effective chromosome 6H resistance gene Rpt5, displays dominant resistance to P. teres f. teres. To genetically characterize P. teres f. teres aviru...
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The American Phytopathological Society
2024-09-01
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| Series: | Molecular Plant-Microbe Interactions |
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-23-0159-R |
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| author | Jinling Li Nathan A. Wyatt Ryan M. Skiba Gayan K. Kariyawasam Jonathan K. Richards Karl Effertz Sajid Rehman Zhaohui Liu Robert S. Brueggeman Timothy L. Friesen |
| author_facet | Jinling Li Nathan A. Wyatt Ryan M. Skiba Gayan K. Kariyawasam Jonathan K. Richards Karl Effertz Sajid Rehman Zhaohui Liu Robert S. Brueggeman Timothy L. Friesen |
| author_sort | Jinling Li |
| collection | DOAJ |
| description | Barley net form net blotch (NFNB) is a destructive foliar disease caused by Pyrenophora teres f. teres. Barley line CIho5791, which harbors the broadly effective chromosome 6H resistance gene Rpt5, displays dominant resistance to P. teres f. teres. To genetically characterize P. teres f. teres avirulence/virulence on the barley line CIho5791, we generated a P. teres f. teres mapping population using a cross between the Moroccan CIho5791-virulent isolate MorSM40-3 and the avirulent reference isolate 0-1. Full genome sequences were generated for 103 progenies. Saturated chromosome-level genetic maps were generated, and quantitative trait locus (QTL) mapping identified two major QTL associated with P. teres f. teres avirulence/virulence on CIho5791. The most significant QTL mapped to chromosome (Ch) 1, where the virulent allele was contributed by MorSM40-3. A second QTL mapped to Ch8; however, this virulent allele was contributed by the avirulent parent 0-1. The Ch1 and Ch8 loci accounted for 27 and 15% of the disease variation, respectively, and the avirulent allele at the Ch1 locus was epistatic over the virulent allele at the Ch8 locus. As a validation, we used a natural P. teres f. teres population in a genome-wide association study that identified the same Ch1 and Ch8 loci. We then generated a new reference quality genome assembly of parental isolate MorSM40-3 with annotation supported by deep transcriptome sequencing of infection time points. The annotation identified candidate genes predicted to encode small, secreted proteins, one or more of which are likely responsible for overcoming the CIho5791 resistance. [Figure: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024. |
| format | Article |
| id | doaj-art-ba2bf95f9bf34def91a7d499416c44b6 |
| institution | OA Journals |
| issn | 0894-0282 1943-7706 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | The American Phytopathological Society |
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| series | Molecular Plant-Microbe Interactions |
| spelling | doaj-art-ba2bf95f9bf34def91a7d499416c44b62025-08-20T01:55:12ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062024-09-0137967668710.1094/MPMI-10-23-0159-RVariability in Chromosome 1 of Select Moroccan Pyrenophora teres f. teres Isolates Overcomes a Highly Effective Barley Chromosome 6H Source of ResistanceJinling Li0Nathan A. Wyatt1Ryan M. Skiba2Gayan K. Kariyawasam3Jonathan K. Richards4Karl Effertz5Sajid Rehman6Zhaohui Liu7Robert S. Brueggeman8Timothy L. Friesen9Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A.Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A.Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Center, USDA-ARS, Fargo, ND 58102, U.S.A.Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A.Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, U.S.A.Department of Crop and Soil Science, Washington State University, Pullman, WA 99164, U.S.A.Biodiversity and Crop Improvement Program, International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat 10100, MoroccoDepartment of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A.Department of Crop and Soil Science, Washington State University, Pullman, WA 99164, U.S.A.Department of Plant Pathology, North Dakota State University, Fargo, ND 58102, U.S.A.Barley net form net blotch (NFNB) is a destructive foliar disease caused by Pyrenophora teres f. teres. Barley line CIho5791, which harbors the broadly effective chromosome 6H resistance gene Rpt5, displays dominant resistance to P. teres f. teres. To genetically characterize P. teres f. teres avirulence/virulence on the barley line CIho5791, we generated a P. teres f. teres mapping population using a cross between the Moroccan CIho5791-virulent isolate MorSM40-3 and the avirulent reference isolate 0-1. Full genome sequences were generated for 103 progenies. Saturated chromosome-level genetic maps were generated, and quantitative trait locus (QTL) mapping identified two major QTL associated with P. teres f. teres avirulence/virulence on CIho5791. The most significant QTL mapped to chromosome (Ch) 1, where the virulent allele was contributed by MorSM40-3. A second QTL mapped to Ch8; however, this virulent allele was contributed by the avirulent parent 0-1. The Ch1 and Ch8 loci accounted for 27 and 15% of the disease variation, respectively, and the avirulent allele at the Ch1 locus was epistatic over the virulent allele at the Ch8 locus. As a validation, we used a natural P. teres f. teres population in a genome-wide association study that identified the same Ch1 and Ch8 loci. We then generated a new reference quality genome assembly of parental isolate MorSM40-3 with annotation supported by deep transcriptome sequencing of infection time points. The annotation identified candidate genes predicted to encode small, secreted proteins, one or more of which are likely responsible for overcoming the CIho5791 resistance. [Figure: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024.https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-23-0159-Ravirulencebarleygenome-wide association mappingnet form net blotchPyrenophora teres f. teresQTL mapping |
| spellingShingle | Jinling Li Nathan A. Wyatt Ryan M. Skiba Gayan K. Kariyawasam Jonathan K. Richards Karl Effertz Sajid Rehman Zhaohui Liu Robert S. Brueggeman Timothy L. Friesen Variability in Chromosome 1 of Select Moroccan Pyrenophora teres f. teres Isolates Overcomes a Highly Effective Barley Chromosome 6H Source of Resistance Molecular Plant-Microbe Interactions avirulence barley genome-wide association mapping net form net blotch Pyrenophora teres f. teres QTL mapping |
| title | Variability in Chromosome 1 of Select Moroccan Pyrenophora teres f. teres Isolates Overcomes a Highly Effective Barley Chromosome 6H Source of Resistance |
| title_full | Variability in Chromosome 1 of Select Moroccan Pyrenophora teres f. teres Isolates Overcomes a Highly Effective Barley Chromosome 6H Source of Resistance |
| title_fullStr | Variability in Chromosome 1 of Select Moroccan Pyrenophora teres f. teres Isolates Overcomes a Highly Effective Barley Chromosome 6H Source of Resistance |
| title_full_unstemmed | Variability in Chromosome 1 of Select Moroccan Pyrenophora teres f. teres Isolates Overcomes a Highly Effective Barley Chromosome 6H Source of Resistance |
| title_short | Variability in Chromosome 1 of Select Moroccan Pyrenophora teres f. teres Isolates Overcomes a Highly Effective Barley Chromosome 6H Source of Resistance |
| title_sort | variability in chromosome 1 of select moroccan pyrenophora teres f teres isolates overcomes a highly effective barley chromosome 6h source of resistance |
| topic | avirulence barley genome-wide association mapping net form net blotch Pyrenophora teres f. teres QTL mapping |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-10-23-0159-R |
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