IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway
Spontaneous preterm birth (sPTB), defined as delivery before 37 weeks of gestation, is thought to be a multifactorial syndrome. However, the inflammatory imbalance at the maternal-fetal interface promotes excessive secretion of inflammatory factors and induces apoptosis and degradation of the extrac...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/1069563 |
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| author | Lulu Wang Zheng Liu Dongni Huang Yuxin Ran Hanwen Zhang Jie He Nanlin Yin Hongbo Qi |
| author_facet | Lulu Wang Zheng Liu Dongni Huang Yuxin Ran Hanwen Zhang Jie He Nanlin Yin Hongbo Qi |
| author_sort | Lulu Wang |
| collection | DOAJ |
| description | Spontaneous preterm birth (sPTB), defined as delivery before 37 weeks of gestation, is thought to be a multifactorial syndrome. However, the inflammatory imbalance at the maternal-fetal interface promotes excessive secretion of inflammatory factors and induces apoptosis and degradation of the extracellular matrix (ECM), which can subsequently lead to preterm birth. As an anti-inflammatory molecule in the IL-1 family, interleukin-37 (IL-37) mainly plays an inhibiting role in a variety of inflammatory diseases. However, as a typical inflammatory disease, no previous studies have been carried out to explore the role of IL-37 in sPTB. In this study, a series of molecular biological experiments were performed in clinical samples and human amniotic epithelial cell line (Wistar Institute Susan Hayflick (WISH)) to investigate the deficiency role of IL-37 and the potential mechanism. Firstly, the results indicated that the expression of IL-37 in human peripheral plasma and fetal membranes was significantly decreased in the sPTB group. Afterward, it is proved that IL-37 could significantly suppress the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in WISH cells. Simultaneously, once silence IL-37, LPS-induced apoptosis and activity of matrix metalloproteinases (MMPs) 2 and 9 were significantly increased. In addition, the western blot data showed that IL-37 performed its biological effects by inhibiting the NF-κB and IL-6/STAT3 pathway. In conclusion, our results suggest that IL-37 limits excessive inflammation and subsequently inhibits ECM remodeling and apoptosis through the NF-κB and IL-6/STAT3 signaling pathway in the fetal membranes. |
| format | Article |
| id | doaj-art-ba287bfb36e94819ba06775802b53ff2 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ba287bfb36e94819ba06775802b53ff22025-08-20T02:19:26ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/10695631069563IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling PathwayLulu Wang0Zheng Liu1Dongni Huang2Yuxin Ran3Hanwen Zhang4Jie He5Nanlin Yin6Hongbo Qi7Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaSpontaneous preterm birth (sPTB), defined as delivery before 37 weeks of gestation, is thought to be a multifactorial syndrome. However, the inflammatory imbalance at the maternal-fetal interface promotes excessive secretion of inflammatory factors and induces apoptosis and degradation of the extracellular matrix (ECM), which can subsequently lead to preterm birth. As an anti-inflammatory molecule in the IL-1 family, interleukin-37 (IL-37) mainly plays an inhibiting role in a variety of inflammatory diseases. However, as a typical inflammatory disease, no previous studies have been carried out to explore the role of IL-37 in sPTB. In this study, a series of molecular biological experiments were performed in clinical samples and human amniotic epithelial cell line (Wistar Institute Susan Hayflick (WISH)) to investigate the deficiency role of IL-37 and the potential mechanism. Firstly, the results indicated that the expression of IL-37 in human peripheral plasma and fetal membranes was significantly decreased in the sPTB group. Afterward, it is proved that IL-37 could significantly suppress the production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in WISH cells. Simultaneously, once silence IL-37, LPS-induced apoptosis and activity of matrix metalloproteinases (MMPs) 2 and 9 were significantly increased. In addition, the western blot data showed that IL-37 performed its biological effects by inhibiting the NF-κB and IL-6/STAT3 pathway. In conclusion, our results suggest that IL-37 limits excessive inflammation and subsequently inhibits ECM remodeling and apoptosis through the NF-κB and IL-6/STAT3 signaling pathway in the fetal membranes.http://dx.doi.org/10.1155/2020/1069563 |
| spellingShingle | Lulu Wang Zheng Liu Dongni Huang Yuxin Ran Hanwen Zhang Jie He Nanlin Yin Hongbo Qi IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway Mediators of Inflammation |
| title | IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway |
| title_full | IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway |
| title_fullStr | IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway |
| title_full_unstemmed | IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway |
| title_short | IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway |
| title_sort | il 37 exerts anti inflammatory effects in fetal membranes of spontaneous preterm birth via the nf κb and il 6 stat3 signaling pathway |
| url | http://dx.doi.org/10.1155/2020/1069563 |
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