pH shift extraction technique for plant proteins: A promising technique for sustainable development
With the recognition of animal-derived foods as unhealthy and their association with climate change, researchers are increasingly focusing on plant-based protein as a sustainable alternative. Plant proteins offer versatile functional and dietary benefits, making them suitable for various food applic...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Energy Nexus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772427124000603 |
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| author | Zainab Sultan Alweera Ashfaq Kausar Jahan Ovais Shafiq Qadri Kaiser Younis Owais Yousuf |
| author_facet | Zainab Sultan Alweera Ashfaq Kausar Jahan Ovais Shafiq Qadri Kaiser Younis Owais Yousuf |
| author_sort | Zainab Sultan |
| collection | DOAJ |
| description | With the recognition of animal-derived foods as unhealthy and their association with climate change, researchers are increasingly focusing on plant-based protein as a sustainable alternative. Plant proteins offer versatile functional and dietary benefits, making them suitable for various food applications. This study investigates the influence of alkaline and acidic pH conditions on the extraction yield and the functional and nutritional properties of plant-based proteins. The primary sources of plant protein include cereals, legumes, and oilseeds, which can be used to address essential amino acid deficiencies through blending. Several methods have been employed for protein extraction from plant sources, such as salt extraction, microwave-assisted extraction, ultrasound-assisted extraction, and micellar precipitation. Among these techniques, the pH shift method stands out due to its non-thermal nature, sustainability, and cost-effectiveness. In this method, proteins are solubilized at alkaline pH and then precipitated at their isoelectric point, resulting in a collection of protein precipitate. It is crucial to optimize extraction techniques based on qualitative and quantitative analysis to enhance protein yield, characteristics, and nutritional value. Most conventional protein extraction methods require a large quantity of chemicals, which imposes the issue of safe disposal, compromising environmental sustainability. Traditional methods also produce protein with non-proteinaceous constituents, adding another purification step and resulting in increased overall cost. However, the pH shift method utilizes comparatively less harsh chemicals and has a high protein extraction yield, which makes it comparatively more environmentally and economically sustainable. The sustainable extraction of plant-based proteins addresses the health and environmental concerns associated with animal-derived foods and offers a promising solution to promote sustainability in the food industry. |
| format | Article |
| id | doaj-art-ba2268f4ecbd4e8a9e8e7145ec134721 |
| institution | OA Journals |
| issn | 2772-4271 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Energy Nexus |
| spelling | doaj-art-ba2268f4ecbd4e8a9e8e7145ec1347212025-08-20T02:38:39ZengElsevierEnergy Nexus2772-42712024-12-011610032910.1016/j.nexus.2024.100329pH shift extraction technique for plant proteins: A promising technique for sustainable developmentZainab Sultan0Alweera Ashfaq1Kausar Jahan2Ovais Shafiq Qadri3Kaiser Younis4Owais Yousuf5Department of Bioengineering, Integral University, Lucknow, IndiaFood Science Department, College of Agriculture and Veterinary Medicine, United Arab Emirates University, United Arab EmiratesDepartment of Bioengineering, Integral University, Lucknow, IndiaDepartment of Biotechnology, Thapar Institute of Engineering & Technology, IndiaDepartment of Food Technology, Islamic University of Science & Technology, IndiaDepartment of Food Technology, Islamic University of Science & Technology, India; Corresponding author.With the recognition of animal-derived foods as unhealthy and their association with climate change, researchers are increasingly focusing on plant-based protein as a sustainable alternative. Plant proteins offer versatile functional and dietary benefits, making them suitable for various food applications. This study investigates the influence of alkaline and acidic pH conditions on the extraction yield and the functional and nutritional properties of plant-based proteins. The primary sources of plant protein include cereals, legumes, and oilseeds, which can be used to address essential amino acid deficiencies through blending. Several methods have been employed for protein extraction from plant sources, such as salt extraction, microwave-assisted extraction, ultrasound-assisted extraction, and micellar precipitation. Among these techniques, the pH shift method stands out due to its non-thermal nature, sustainability, and cost-effectiveness. In this method, proteins are solubilized at alkaline pH and then precipitated at their isoelectric point, resulting in a collection of protein precipitate. It is crucial to optimize extraction techniques based on qualitative and quantitative analysis to enhance protein yield, characteristics, and nutritional value. Most conventional protein extraction methods require a large quantity of chemicals, which imposes the issue of safe disposal, compromising environmental sustainability. Traditional methods also produce protein with non-proteinaceous constituents, adding another purification step and resulting in increased overall cost. However, the pH shift method utilizes comparatively less harsh chemicals and has a high protein extraction yield, which makes it comparatively more environmentally and economically sustainable. The sustainable extraction of plant-based proteins addresses the health and environmental concerns associated with animal-derived foods and offers a promising solution to promote sustainability in the food industry.http://www.sciencedirect.com/science/article/pii/S2772427124000603Plant proteinpH shift methodIsoelectric pointFunctional propertiesNutritional properties |
| spellingShingle | Zainab Sultan Alweera Ashfaq Kausar Jahan Ovais Shafiq Qadri Kaiser Younis Owais Yousuf pH shift extraction technique for plant proteins: A promising technique for sustainable development Energy Nexus Plant protein pH shift method Isoelectric point Functional properties Nutritional properties |
| title | pH shift extraction technique for plant proteins: A promising technique for sustainable development |
| title_full | pH shift extraction technique for plant proteins: A promising technique for sustainable development |
| title_fullStr | pH shift extraction technique for plant proteins: A promising technique for sustainable development |
| title_full_unstemmed | pH shift extraction technique for plant proteins: A promising technique for sustainable development |
| title_short | pH shift extraction technique for plant proteins: A promising technique for sustainable development |
| title_sort | ph shift extraction technique for plant proteins a promising technique for sustainable development |
| topic | Plant protein pH shift method Isoelectric point Functional properties Nutritional properties |
| url | http://www.sciencedirect.com/science/article/pii/S2772427124000603 |
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