Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal
A rapid, simple, and novel method for folate receptor α (FRα) determination is reported here. A probe of gold nanoparticles (Au NPs) modified with anti-FRα antibody was synthesized under the optimized conditions first. The antibody-modified Au NPs would aggregate when FRα was added to the probe for...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/1670812 |
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| author | Liping Wu Yue Liu Rong Huang Huawen Zhao Weiqun Shu |
| author_facet | Liping Wu Yue Liu Rong Huang Huawen Zhao Weiqun Shu |
| author_sort | Liping Wu |
| collection | DOAJ |
| description | A rapid, simple, and novel method for folate receptor α (FRα) determination is reported here. A probe of gold nanoparticles (Au NPs) modified with anti-FRα antibody was synthesized under the optimized conditions first. The antibody-modified Au NPs would aggregate when FRα was added to the probe for the specific interaction between antibody and antigen, resulting in the enhancement of resonance Rayleigh scattering (RRS) intensity. There is a linear relationship between the change of RRS intensity (ΔIRRS) and the concentration of FRα, with the detecting range of 0.50–37.50 ng·mL−1 and the limit of determination of 0.05 ng·mL−1. The determination of FRα in serum samples was realized with the advantages of high selectivity, high sensitivity, and easy operation. |
| format | Article |
| id | doaj-art-ba1f2ae586344358b803baef98a85dac |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-ba1f2ae586344358b803baef98a85dac2025-02-03T05:46:53ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792017-01-01201710.1155/2017/16708121670812Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering SignalLiping Wu0Yue Liu1Rong Huang2Huawen Zhao3Weiqun Shu4Department of Environmental Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, ChinaDepartment of Chemistry, College of Pharmacy, Third Military Medical University, Chongqing 400038, ChinaDepartment of Chemistry, College of Pharmacy, Third Military Medical University, Chongqing 400038, ChinaDepartment of Chemistry, College of Pharmacy, Third Military Medical University, Chongqing 400038, ChinaDepartment of Environmental Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, ChinaA rapid, simple, and novel method for folate receptor α (FRα) determination is reported here. A probe of gold nanoparticles (Au NPs) modified with anti-FRα antibody was synthesized under the optimized conditions first. The antibody-modified Au NPs would aggregate when FRα was added to the probe for the specific interaction between antibody and antigen, resulting in the enhancement of resonance Rayleigh scattering (RRS) intensity. There is a linear relationship between the change of RRS intensity (ΔIRRS) and the concentration of FRα, with the detecting range of 0.50–37.50 ng·mL−1 and the limit of determination of 0.05 ng·mL−1. The determination of FRα in serum samples was realized with the advantages of high selectivity, high sensitivity, and easy operation.http://dx.doi.org/10.1155/2017/1670812 |
| spellingShingle | Liping Wu Yue Liu Rong Huang Huawen Zhao Weiqun Shu Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal International Journal of Analytical Chemistry |
| title | Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal |
| title_full | Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal |
| title_fullStr | Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal |
| title_full_unstemmed | Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal |
| title_short | Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal |
| title_sort | rapid and selective determination of folate receptor α with sensitive resonance rayleigh scattering signal |
| url | http://dx.doi.org/10.1155/2017/1670812 |
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