Protocol to assess the impact of sperm RNA on mouse preimplantation embryos using microinjection

Protocol to assess the impact of sperm RNA on mouse preimplantation embryos using microinjection

Summary: Zygotic microinjections show that sperm RNAs transmit non-genetically inherited phenotypes to offspring and influence embryonic development. Here, we present a protocol for the micromanipulation of mouse zygotes to introduce physiologically relevant levels of sperm RNA. We describe steps fo...

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Bibliographic Details
Main Authors: Grace S. Lee, Natalie A. Trigg, Colin C. Conine
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Developmental biology
Gene Expression
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001789
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