Chitosan nanoencapsulation of Turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic rats
Abstract Cisplatin is a chemotherapeutic drug, which exhibits undesirable side effects. Chitosan nanoparticles are promising for drug delivery. The aim of this study was to determine the effect of the brown alga Turbinaria triquetra ethyl acetate fraction and polysaccharides, either loaded on chitos...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-82463-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559620348280832 |
|---|---|
| author | Abdullah Abdel-Aal Abdel-Aziz A. F. Zakaria El-khayat Nadia Mohamed Merit Rostom Emad Tolba Nihal Galal El-Din Shams El-Din Walaa S. A. Mettwally Abdelhamid Aly Hamdy |
| author_facet | Abdullah Abdel-Aal Abdel-Aziz A. F. Zakaria El-khayat Nadia Mohamed Merit Rostom Emad Tolba Nihal Galal El-Din Shams El-Din Walaa S. A. Mettwally Abdelhamid Aly Hamdy |
| author_sort | Abdullah Abdel-Aal |
| collection | DOAJ |
| description | Abstract Cisplatin is a chemotherapeutic drug, which exhibits undesirable side effects. Chitosan nanoparticles are promising for drug delivery. The aim of this study was to determine the effect of the brown alga Turbinaria triquetra ethyl acetate fraction and polysaccharides, either loaded on chitosan nanoparticles or free, against podocyturia and cisplatin nephrotoxicity in rats. Sixty-six male rats were distributed into 11 equal groups: untreated control, chitosan (CSNPs), ethyl acetate fraction (EAE), polysaccharide (PS), EAE loaded on chitosan nanoparticles (EAE-CSNPs), PS loaded on chitosan nanoparticles (PS-CSNPs), Cisplatin or cis-diamminedichloroplatinum(II) (CDDP), CDDP + EAE, CDDP + PS, CDDP + EAE-CSNPs, and CDDP + PS-CSNPs. Serum urea, creatinine, creatinine clearance, renal malondialdehyde, nitric oxide, paraoxonase 1, renal nephrin, and podocin, and their renal mRNA gene expressions, as well as urinary nephrin and podocin were determined. The results indicated that the ethyl acetate fraction and polysaccharides, either free or loaded, efficiently attenuated podocyturia and cisplatin nephrotoxicity compared to the Cis group. However, the improvement was higher in the nephrotoxic groups treated with EAE-CSNPs and PS-CSNPs. The current study revealed that chitosan nanoencapsulation showed ameliorative effects against podocyturia and cisplatin nephrotoxicity in rats compared to free extracts, offering a new therapeutic strategy for attenuating podocyturia and CDDP-induced nephrotoxicity. |
| format | Article |
| id | doaj-art-ba07bd22d26249c482c760bc8f1d5026 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ba07bd22d26249c482c760bc8f1d50262025-01-05T12:22:24ZengNature PortfolioScientific Reports2045-23222025-01-0115111610.1038/s41598-024-82463-yChitosan nanoencapsulation of Turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic ratsAbdullah Abdel-Aal0Abdel-Aziz A. F.1Zakaria El-khayat2Nadia Mohamed3Merit Rostom4Emad Tolba5Nihal Galal El-Din Shams El-Din6Walaa S. A. Mettwally7Abdelhamid Aly Hamdy8Biochemistry Division, Faculty of Science, Mansoura UniversityBiochemistry Division, Faculty of Science, Mansoura UniversityMedical Biochemistry Department, National Research CentreMedical Biochemistry Department, National Research CentreAcademy of Scientific Research and Technology, ASRTPolymers and Pigments Department, National Research CentreNational Institute of Oceanography and Fisheries (NIOF)Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute , National Research CentreChemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute , National Research CentreAbstract Cisplatin is a chemotherapeutic drug, which exhibits undesirable side effects. Chitosan nanoparticles are promising for drug delivery. The aim of this study was to determine the effect of the brown alga Turbinaria triquetra ethyl acetate fraction and polysaccharides, either loaded on chitosan nanoparticles or free, against podocyturia and cisplatin nephrotoxicity in rats. Sixty-six male rats were distributed into 11 equal groups: untreated control, chitosan (CSNPs), ethyl acetate fraction (EAE), polysaccharide (PS), EAE loaded on chitosan nanoparticles (EAE-CSNPs), PS loaded on chitosan nanoparticles (PS-CSNPs), Cisplatin or cis-diamminedichloroplatinum(II) (CDDP), CDDP + EAE, CDDP + PS, CDDP + EAE-CSNPs, and CDDP + PS-CSNPs. Serum urea, creatinine, creatinine clearance, renal malondialdehyde, nitric oxide, paraoxonase 1, renal nephrin, and podocin, and their renal mRNA gene expressions, as well as urinary nephrin and podocin were determined. The results indicated that the ethyl acetate fraction and polysaccharides, either free or loaded, efficiently attenuated podocyturia and cisplatin nephrotoxicity compared to the Cis group. However, the improvement was higher in the nephrotoxic groups treated with EAE-CSNPs and PS-CSNPs. The current study revealed that chitosan nanoencapsulation showed ameliorative effects against podocyturia and cisplatin nephrotoxicity in rats compared to free extracts, offering a new therapeutic strategy for attenuating podocyturia and CDDP-induced nephrotoxicity.https://doi.org/10.1038/s41598-024-82463-yBrown algaTurbinaria TriquetraPolysaccharideChitosan nanoparticlesCisplatinNephrotoxicity |
| spellingShingle | Abdullah Abdel-Aal Abdel-Aziz A. F. Zakaria El-khayat Nadia Mohamed Merit Rostom Emad Tolba Nihal Galal El-Din Shams El-Din Walaa S. A. Mettwally Abdelhamid Aly Hamdy Chitosan nanoencapsulation of Turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic rats Scientific Reports Brown alga Turbinaria Triquetra Polysaccharide Chitosan nanoparticles Cisplatin Nephrotoxicity |
| title | Chitosan nanoencapsulation of Turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic rats |
| title_full | Chitosan nanoencapsulation of Turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic rats |
| title_fullStr | Chitosan nanoencapsulation of Turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic rats |
| title_full_unstemmed | Chitosan nanoencapsulation of Turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic rats |
| title_short | Chitosan nanoencapsulation of Turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic rats |
| title_sort | chitosan nanoencapsulation of turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic rats |
| topic | Brown alga Turbinaria Triquetra Polysaccharide Chitosan nanoparticles Cisplatin Nephrotoxicity |
| url | https://doi.org/10.1038/s41598-024-82463-y |
| work_keys_str_mv | AT abdullahabdelaal chitosannanoencapsulationofturbinariatriquetrametabolitesinthemanagementofpodocyturiainnephrotoxicrats AT abdelazizaf chitosannanoencapsulationofturbinariatriquetrametabolitesinthemanagementofpodocyturiainnephrotoxicrats AT zakariaelkhayat chitosannanoencapsulationofturbinariatriquetrametabolitesinthemanagementofpodocyturiainnephrotoxicrats AT nadiamohamed chitosannanoencapsulationofturbinariatriquetrametabolitesinthemanagementofpodocyturiainnephrotoxicrats AT meritrostom chitosannanoencapsulationofturbinariatriquetrametabolitesinthemanagementofpodocyturiainnephrotoxicrats AT emadtolba chitosannanoencapsulationofturbinariatriquetrametabolitesinthemanagementofpodocyturiainnephrotoxicrats AT nihalgalaleldinshamseldin chitosannanoencapsulationofturbinariatriquetrametabolitesinthemanagementofpodocyturiainnephrotoxicrats AT walaasamettwally chitosannanoencapsulationofturbinariatriquetrametabolitesinthemanagementofpodocyturiainnephrotoxicrats AT abdelhamidalyhamdy chitosannanoencapsulationofturbinariatriquetrametabolitesinthemanagementofpodocyturiainnephrotoxicrats |