Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers

The chemical composition of essential oil and volatile obtained from the roots of Jatropha ribifolia (Pohl) Baill was performed in this work. The Clevenger extractor was utilized in hydrodistillation of oil and chemical composition determined by gas chromatography coupled with mass spectrometry det...

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Main Authors: Celia Eliane de Lara da Silva, Willian Ferreira da Costa, Sandro Minguzzi, Rogério Cesar de Lara da Silva, Euclésio Simionatto
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2013/352606
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author Celia Eliane de Lara da Silva
Willian Ferreira da Costa
Sandro Minguzzi
Rogério Cesar de Lara da Silva
Euclésio Simionatto
author_facet Celia Eliane de Lara da Silva
Willian Ferreira da Costa
Sandro Minguzzi
Rogério Cesar de Lara da Silva
Euclésio Simionatto
author_sort Celia Eliane de Lara da Silva
collection DOAJ
description The chemical composition of essential oil and volatile obtained from the roots of Jatropha ribifolia (Pohl) Baill was performed in this work. The Clevenger extractor was utilized in hydrodistillation of oil and chemical composition determined by gas chromatography coupled with mass spectrometry detector (GC-MS). The identification of compounds was confirmed by retention index (Kovats index) obtained from a series of straight chain alkanes (C7–C30) and by comparison with NIST and ADAMS library. A total of 61 compounds were identified in essential oil by GC-MS. The extraction of volatile was performed also by the use of the solid phase microextraction (SPME) with four different fibers. The essential oil extraction was extremely rapid (15 s) to avoid saturation of the fiber and the MS detector. The majority of the composition of essential oil is the terpenes: β-pinene (major compound 9.16%), β-vatirene (8.34%), α-gurjunene (6.98%), α-pinene (6.35%), camphene (4.34%), tricyclene (3.79%) and dehydro aromadendrene (3.52%) it and aldehydes and alcohols. Through the SPME it was possible to determine the nine volatile compounds not identified in oil 2,3,4-trimethyl-2-cyclopenten-1-one, α-phellandrene, 3-carene, trans-p-mentha-2,8-dienol, pinocamphone, D-verbenon, 1,3,3-trimethyl-2-(2-methyl-cyclopropyl)-cyclohexene, 2,4-diisocyanato-1-methylbenzene, and (6-hydroxymethyl-2,3-dimethylehenyl) methanol.
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spelling doaj-art-ba04000cdf4e4b12ac605cdee82591582025-02-03T01:12:57ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/352606352606Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different FibersCelia Eliane de Lara da Silva0Willian Ferreira da Costa1Sandro Minguzzi2Rogério Cesar de Lara da Silva3Euclésio Simionatto4Centro de Pesquisas e Tecnologia em Recursos Naturais (CPTREN), Pós-Gradução em Recursos Naturais (PGRN), Departamento de Química, Universidade Estadual de Mato Grosso do Sul (UEMS), Rua Emílio Mascolli, 275, 79950-000 Naviraí, MS, BrazilComplexos e Centrais de Apoio a Pesquisa (COMCAP), Departamento de Química, Universidade Estadual de Maringá (UEM), Avenida Colombo, 5790, Jd Universitário, 87020-900 Maringá, PR, BrazilCentro de Pesquisas e Tecnologia em Recursos Naturais (CPTREN), Pós-Gradução em Recursos Naturais (PGRN), Departamento de Química, Universidade Estadual de Mato Grosso do Sul (UEMS), Rua Emílio Mascolli, 275, 79950-000 Naviraí, MS, BrazilCentro de Pesquisas e Tecnologia em Recursos Naturais (CPTREN), Pós-Gradução em Recursos Naturais (PGRN), Departamento de Química, Universidade Estadual de Mato Grosso do Sul (UEMS), Rua Emílio Mascolli, 275, 79950-000 Naviraí, MS, BrazilCentro de Pesquisas e Tecnologia em Recursos Naturais (CPTREN), Pós-Gradução em Recursos Naturais (PGRN), Departamento de Química, Universidade Estadual de Mato Grosso do Sul (UEMS), Rua Emílio Mascolli, 275, 79950-000 Naviraí, MS, BrazilThe chemical composition of essential oil and volatile obtained from the roots of Jatropha ribifolia (Pohl) Baill was performed in this work. The Clevenger extractor was utilized in hydrodistillation of oil and chemical composition determined by gas chromatography coupled with mass spectrometry detector (GC-MS). The identification of compounds was confirmed by retention index (Kovats index) obtained from a series of straight chain alkanes (C7–C30) and by comparison with NIST and ADAMS library. A total of 61 compounds were identified in essential oil by GC-MS. The extraction of volatile was performed also by the use of the solid phase microextraction (SPME) with four different fibers. The essential oil extraction was extremely rapid (15 s) to avoid saturation of the fiber and the MS detector. The majority of the composition of essential oil is the terpenes: β-pinene (major compound 9.16%), β-vatirene (8.34%), α-gurjunene (6.98%), α-pinene (6.35%), camphene (4.34%), tricyclene (3.79%) and dehydro aromadendrene (3.52%) it and aldehydes and alcohols. Through the SPME it was possible to determine the nine volatile compounds not identified in oil 2,3,4-trimethyl-2-cyclopenten-1-one, α-phellandrene, 3-carene, trans-p-mentha-2,8-dienol, pinocamphone, D-verbenon, 1,3,3-trimethyl-2-(2-methyl-cyclopropyl)-cyclohexene, 2,4-diisocyanato-1-methylbenzene, and (6-hydroxymethyl-2,3-dimethylehenyl) methanol.http://dx.doi.org/10.1155/2013/352606
spellingShingle Celia Eliane de Lara da Silva
Willian Ferreira da Costa
Sandro Minguzzi
Rogério Cesar de Lara da Silva
Euclésio Simionatto
Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers
Journal of Analytical Methods in Chemistry
title Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers
title_full Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers
title_fullStr Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers
title_full_unstemmed Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers
title_short Assessment of Volatile Chemical Composition of the Essential Oil of Jatropha ribifolia (Pohl) Baill by HS-SPME-GC-MS Using Different Fibers
title_sort assessment of volatile chemical composition of the essential oil of jatropha ribifolia pohl baill by hs spme gc ms using different fibers
url http://dx.doi.org/10.1155/2013/352606
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