Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection
The gut microbiome’s pivotal role in health and disease is well established. SARS-CoV-2 infection often causes gastrointestinal symptoms and is associated with changes of the microbiome in both human and animal studies. While hamsters serve as important animal models for coronavirus research, there...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2025.2505117 |
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| author | Marybeth Creskey Fabiola Silva Angulo Qing Wu Levi Tamming Emily E. F. Fekete Kai Cheng Zhibin Ning Angela Wang Patrícia Brito Rodrigues Vinícius de Rezende Rodovalho Marco Aurélio Ramirez Vinolo Daniel Figeys Xuguang Li Francois Trottein Xu Zhang |
| author_facet | Marybeth Creskey Fabiola Silva Angulo Qing Wu Levi Tamming Emily E. F. Fekete Kai Cheng Zhibin Ning Angela Wang Patrícia Brito Rodrigues Vinícius de Rezende Rodovalho Marco Aurélio Ramirez Vinolo Daniel Figeys Xuguang Li Francois Trottein Xu Zhang |
| author_sort | Marybeth Creskey |
| collection | DOAJ |
| description | The gut microbiome’s pivotal role in health and disease is well established. SARS-CoV-2 infection often causes gastrointestinal symptoms and is associated with changes of the microbiome in both human and animal studies. While hamsters serve as important animal models for coronavirus research, there exists a notable void in the functional characterization of their microbiomes with metaproteomics. In this study, we present a workflow for analyzing the hamster gut microbiome, including a metagenomics-derived hamster gut microbial protein database and a data-independent acquisition metaproteomics method. Using this workflow, we identified 32,419 protein groups from the fecal microbiomes of young and old hamsters infected with SARS-CoV-2. We showed age-specific changes in the expressions of microbiome functions and host proteins associated with microbiomes, providing further functional insight into the interactions between the microbiome and host in SARS-CoV-2 infection. Altogether, this study established and demonstrated the capability of metaproteomics for the study of hamster microbiomes. |
| format | Article |
| id | doaj-art-b9f8a81986d444d4a0fc98855c63f7b7 |
| institution | OA Journals |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-b9f8a81986d444d4a0fc98855c63f7b72025-08-20T01:53:30ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2025.2505117Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infectionMarybeth Creskey0Fabiola Silva Angulo1Qing Wu2Levi Tamming3Emily E. F. Fekete4Kai Cheng5Zhibin Ning6Angela Wang7Patrícia Brito Rodrigues8Vinícius de Rezende Rodovalho9Marco Aurélio Ramirez Vinolo10Daniel Figeys11Xuguang Li12Francois Trottein13Xu Zhang14Regulatory Research Division, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada, Ottawa, CanadaUniversity of Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, FranceSchool of Pharmaceutical Sciences, Faculty of Medicine, University of Ottawa, Ottawa, CanadaRegulatory Research Division, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada, Ottawa, CanadaRegulatory Research Division, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada, Ottawa, CanadaSchool of Pharmaceutical Sciences, Faculty of Medicine, University of Ottawa, Ottawa, CanadaSchool of Pharmaceutical Sciences, Faculty of Medicine, University of Ottawa, Ottawa, CanadaRegulatory Research Division, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada, Ottawa, CanadaUniversity of Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, FranceLaboratory of Immunoinflammation, Institute of Biology, University of Campinas (UNICAMP), Campinas, BrazilLaboratory of Immunoinflammation, Institute of Biology, University of Campinas (UNICAMP), Campinas, BrazilSchool of Pharmaceutical Sciences, Faculty of Medicine, University of Ottawa, Ottawa, CanadaRegulatory Research Division, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada, Ottawa, CanadaUniversity of Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, Lille, FranceRegulatory Research Division, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada, Ottawa, CanadaThe gut microbiome’s pivotal role in health and disease is well established. SARS-CoV-2 infection often causes gastrointestinal symptoms and is associated with changes of the microbiome in both human and animal studies. While hamsters serve as important animal models for coronavirus research, there exists a notable void in the functional characterization of their microbiomes with metaproteomics. In this study, we present a workflow for analyzing the hamster gut microbiome, including a metagenomics-derived hamster gut microbial protein database and a data-independent acquisition metaproteomics method. Using this workflow, we identified 32,419 protein groups from the fecal microbiomes of young and old hamsters infected with SARS-CoV-2. We showed age-specific changes in the expressions of microbiome functions and host proteins associated with microbiomes, providing further functional insight into the interactions between the microbiome and host in SARS-CoV-2 infection. Altogether, this study established and demonstrated the capability of metaproteomics for the study of hamster microbiomes.https://www.tandfonline.com/doi/10.1080/19490976.2025.2505117Metaproteomicsgut microbiomedata-independent acquisitionCOVID-19 |
| spellingShingle | Marybeth Creskey Fabiola Silva Angulo Qing Wu Levi Tamming Emily E. F. Fekete Kai Cheng Zhibin Ning Angela Wang Patrícia Brito Rodrigues Vinícius de Rezende Rodovalho Marco Aurélio Ramirez Vinolo Daniel Figeys Xuguang Li Francois Trottein Xu Zhang Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection Gut Microbes Metaproteomics gut microbiome data-independent acquisition COVID-19 |
| title | Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection |
| title_full | Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection |
| title_fullStr | Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection |
| title_full_unstemmed | Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection |
| title_short | Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection |
| title_sort | metaproteomics reveals age specific alterations of gut microbiome in hamsters with sars cov 2 infection |
| topic | Metaproteomics gut microbiome data-independent acquisition COVID-19 |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2025.2505117 |
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