Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.

MYB family genes are widely distributed in plants and comprise one of the largest transcription factors involved in various developmental processes and defense responses of plants. To date, few MYB genes and little expression profiling have been reported for citrus. Here, we describe and classify 17...

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Main Authors: Xiao-Jin Hou, Si-Bei Li, Sheng-Rui Liu, Chun-Gen Hu, Jin-Zhi Zhang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0112375&type=printable
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author Xiao-Jin Hou
Si-Bei Li
Sheng-Rui Liu
Chun-Gen Hu
Jin-Zhi Zhang
author_facet Xiao-Jin Hou
Si-Bei Li
Sheng-Rui Liu
Chun-Gen Hu
Jin-Zhi Zhang
author_sort Xiao-Jin Hou
collection DOAJ
description MYB family genes are widely distributed in plants and comprise one of the largest transcription factors involved in various developmental processes and defense responses of plants. To date, few MYB genes and little expression profiling have been reported for citrus. Here, we describe and classify 177 members of the sweet orange MYB gene (CsMYB) family in terms of their genomic gene structures and similarity to their putative Arabidopsis orthologs. According to these analyses, these CsMYBs were categorized into four groups (4R-MYB, 3R-MYB, 2R-MYB and 1R-MYB). Gene structure analysis revealed that 1R-MYB genes possess relatively more introns as compared with 2R-MYB genes. Investigation of their chromosomal localizations revealed that these CsMYBs are distributed across nine chromosomes. Sweet orange includes a relatively small number of MYB genes compared with the 198 members in Arabidopsis, presumably due to a paralog reduction related to repetitive sequence insertion into promoter and non-coding transcribed region of the genes. Comparative studies of CsMYBs and Arabidopsis showed that CsMYBs had fewer gene duplication events. Expression analysis revealed that the MYB gene family has a wide expression profile in sweet orange development and plays important roles in development and stress responses. In addition, 337 new putative microsatellites with flanking sequences sufficient for primer design were also identified from the 177 CsMYBs. These results provide a useful reference for the selection of candidate MYB genes for cloning and further functional analysis forcitrus.
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publisher Public Library of Science (PLoS)
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spelling doaj-art-b9f0f3e620574320aa3822ed3c3cfb5b2025-08-20T03:01:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11237510.1371/journal.pone.0112375Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.Xiao-Jin HouSi-Bei LiSheng-Rui LiuChun-Gen HuJin-Zhi ZhangMYB family genes are widely distributed in plants and comprise one of the largest transcription factors involved in various developmental processes and defense responses of plants. To date, few MYB genes and little expression profiling have been reported for citrus. Here, we describe and classify 177 members of the sweet orange MYB gene (CsMYB) family in terms of their genomic gene structures and similarity to their putative Arabidopsis orthologs. According to these analyses, these CsMYBs were categorized into four groups (4R-MYB, 3R-MYB, 2R-MYB and 1R-MYB). Gene structure analysis revealed that 1R-MYB genes possess relatively more introns as compared with 2R-MYB genes. Investigation of their chromosomal localizations revealed that these CsMYBs are distributed across nine chromosomes. Sweet orange includes a relatively small number of MYB genes compared with the 198 members in Arabidopsis, presumably due to a paralog reduction related to repetitive sequence insertion into promoter and non-coding transcribed region of the genes. Comparative studies of CsMYBs and Arabidopsis showed that CsMYBs had fewer gene duplication events. Expression analysis revealed that the MYB gene family has a wide expression profile in sweet orange development and plays important roles in development and stress responses. In addition, 337 new putative microsatellites with flanking sequences sufficient for primer design were also identified from the 177 CsMYBs. These results provide a useful reference for the selection of candidate MYB genes for cloning and further functional analysis forcitrus.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0112375&type=printable
spellingShingle Xiao-Jin Hou
Si-Bei Li
Sheng-Rui Liu
Chun-Gen Hu
Jin-Zhi Zhang
Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.
PLoS ONE
title Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.
title_full Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.
title_fullStr Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.
title_full_unstemmed Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.
title_short Genome-wide classification and evolutionary and expression analyses of citrus MYB transcription factor families in sweet orange.
title_sort genome wide classification and evolutionary and expression analyses of citrus myb transcription factor families in sweet orange
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0112375&type=printable
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