Effect of polysaccharide fractions from Fortunella margarita on the fecal microbiota of mice and SCFA production in vitro
Fortunella margarita polysaccharides (FMPS) are composed of 4 polysaccharide fragments, namely mannogalactoglucan, galactoglucan, glucan and arabinoglucan. This study investigated the in vitro fermentation of FMPS fractions and their effect on the fecal microbiota of mice and short chain fatty acids...
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Tsinghua University Press
2022-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302100077X |
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| author | Peilin Chen Suzhen Lei Mingyao Tong Qing Chang Baodong Zheng Yi Zhang Hongliang Zeng |
| author_facet | Peilin Chen Suzhen Lei Mingyao Tong Qing Chang Baodong Zheng Yi Zhang Hongliang Zeng |
| author_sort | Peilin Chen |
| collection | DOAJ |
| description | Fortunella margarita polysaccharides (FMPS) are composed of 4 polysaccharide fragments, namely mannogalactoglucan, galactoglucan, glucan and arabinoglucan. This study investigated the in vitro fermentation of FMPS fractions and their effect on the fecal microbiota of mice and short chain fatty acids (SCFAs) production. The results showed that the structure of the fecal microbiota was changed after FMPS fermentation. At the phylum level, the polysaccharide fractions reduced the relative abundance of Bacteroidetes compared with the inulin group. At the genus level, the polysaccharide fractions increased the abundance of Lactobacillus, and decreased the abundance of Granulicatella, Proteus and Rummeliibacillus. Polysaccharide fractions could promote the proliferation of beneficial bacteria (Lactobacillus), and decreased harmful bacteria (Granulicatella, Proteus, Rummeliibacillus and Serratia). Furthermore, there was a significant distinction in the genus level flora processed by different polysaccharide fractions, especially mannogalactoglucan. Polysaccharide fractions showed potential prebiotic effects, with mannogalactoglucan, in particular, promoting proliferation of SCFA-producing bacteria. SCFAs were regulated by monosaccharide composition, molecular weight of the FMPS fractions, and the composition of the fecal bacteria. Polysaccharide fractions could modulate metabolic function of the fecal microbiota, altering the levels of SCFAs. These results suggested that FMPS are important functional components in gut health, especially mannogalactoglucan. |
| format | Article |
| id | doaj-art-b9e6d85e9dca42649a71d79790a50659 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-b9e6d85e9dca42649a71d79790a506592025-02-03T04:08:14ZengTsinghua University PressFood Science and Human Wellness2213-45302022-01-0111197108Effect of polysaccharide fractions from Fortunella margarita on the fecal microbiota of mice and SCFA production in vitroPeilin Chen0Suzhen Lei1Mingyao Tong2Qing Chang3Baodong Zheng4Yi Zhang5Hongliang Zeng6College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China; China-Ireland International Cooperation Centre for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China; China-Ireland International Cooperation Centre for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Quality Science and Processing Technology in Special Starch, Fujian Agriculture and Forestry University, Fuzhou 350002, China; China-Ireland International Cooperation Centre for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Corresponding author at: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.Fortunella margarita polysaccharides (FMPS) are composed of 4 polysaccharide fragments, namely mannogalactoglucan, galactoglucan, glucan and arabinoglucan. This study investigated the in vitro fermentation of FMPS fractions and their effect on the fecal microbiota of mice and short chain fatty acids (SCFAs) production. The results showed that the structure of the fecal microbiota was changed after FMPS fermentation. At the phylum level, the polysaccharide fractions reduced the relative abundance of Bacteroidetes compared with the inulin group. At the genus level, the polysaccharide fractions increased the abundance of Lactobacillus, and decreased the abundance of Granulicatella, Proteus and Rummeliibacillus. Polysaccharide fractions could promote the proliferation of beneficial bacteria (Lactobacillus), and decreased harmful bacteria (Granulicatella, Proteus, Rummeliibacillus and Serratia). Furthermore, there was a significant distinction in the genus level flora processed by different polysaccharide fractions, especially mannogalactoglucan. Polysaccharide fractions showed potential prebiotic effects, with mannogalactoglucan, in particular, promoting proliferation of SCFA-producing bacteria. SCFAs were regulated by monosaccharide composition, molecular weight of the FMPS fractions, and the composition of the fecal bacteria. Polysaccharide fractions could modulate metabolic function of the fecal microbiota, altering the levels of SCFAs. These results suggested that FMPS are important functional components in gut health, especially mannogalactoglucan.http://www.sciencedirect.com/science/article/pii/S221345302100077XPolysaccharide fractionFortunella margaritaFermentationFecal microbiotaShort chain fatty acid |
| spellingShingle | Peilin Chen Suzhen Lei Mingyao Tong Qing Chang Baodong Zheng Yi Zhang Hongliang Zeng Effect of polysaccharide fractions from Fortunella margarita on the fecal microbiota of mice and SCFA production in vitro Food Science and Human Wellness Polysaccharide fraction Fortunella margarita Fermentation Fecal microbiota Short chain fatty acid |
| title | Effect of polysaccharide fractions from Fortunella margarita on the fecal microbiota of mice and SCFA production in vitro |
| title_full | Effect of polysaccharide fractions from Fortunella margarita on the fecal microbiota of mice and SCFA production in vitro |
| title_fullStr | Effect of polysaccharide fractions from Fortunella margarita on the fecal microbiota of mice and SCFA production in vitro |
| title_full_unstemmed | Effect of polysaccharide fractions from Fortunella margarita on the fecal microbiota of mice and SCFA production in vitro |
| title_short | Effect of polysaccharide fractions from Fortunella margarita on the fecal microbiota of mice and SCFA production in vitro |
| title_sort | effect of polysaccharide fractions from fortunella margarita on the fecal microbiota of mice and scfa production in vitro |
| topic | Polysaccharide fraction Fortunella margarita Fermentation Fecal microbiota Short chain fatty acid |
| url | http://www.sciencedirect.com/science/article/pii/S221345302100077X |
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