Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol

Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol

Single-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus presen...

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Bibliographic Details
Main Authors: Firoozeh V Gerayeli, Stephen Milne, Chen Xi Yang, Xuan Li, Elizabeth Guinto, Julia Shun-Wei Yang, Chung Yan Cheung, Tara R Stach, Tawimas Shaipanich, Janice M Leung, Don D Sin
Format: Article
Language:English
Published: Taylor & Francis Group 2023-10-01
Series:BioTechniques
Subjects:
airway diseases
bronchial brush
bronchoalveolar lavage
scRNA-seq
single-cell RNA sequencing
single-cell suspension
Online Access:https://www.future-science.com/doi/10.2144/btn-2023-0017
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