Greater Endothelial Apoptosis and Oxidative Stress in Patients with Peripheral Artery Disease
We compared apoptosis, cellular oxidative stress, and inflammation of cultured endothelial cells treated with sera from 156 subjects with peripheral artery disease (PAD) and 16 healthy control subjects. Furthermore, we compared circulating inflammatory, antioxidant capacity, and vascular biomarkers...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | International Journal of Vascular Medicine |
| Online Access: | http://dx.doi.org/10.1155/2014/160534 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850177616039903232 |
|---|---|
| author | Andrew W. Gardner Donald E. Parker Polly S. Montgomery Danuta Sosnowska Ana I. Casanegra Zoltan Ungvari Anna Csiszar William E. Sonntag |
| author_facet | Andrew W. Gardner Donald E. Parker Polly S. Montgomery Danuta Sosnowska Ana I. Casanegra Zoltan Ungvari Anna Csiszar William E. Sonntag |
| author_sort | Andrew W. Gardner |
| collection | DOAJ |
| description | We compared apoptosis, cellular oxidative stress, and inflammation of cultured endothelial cells treated with sera from 156 subjects with peripheral artery disease (PAD) and 16 healthy control subjects. Furthermore, we compared circulating inflammatory, antioxidant capacity, and vascular biomarkers between the two groups. The PAD group had a 164% higher value for endothelial cell apoptosis (P<0.001) and a 62% higher value for endothelial cellular reactive oxygen species production (P<0.001) than the control group. Furthermore, the PAD group had lower systemic antioxidant capacity measured by hydroxyl radical antioxidant capacity activity (P<0.001), higher inflammatory and vascular measures of high-sensitivity C-reactive protein (P<0.001), interleukin-8 (P<0.001), serum amyloid A (P<0.001), vascular cell adhesion molecule-1 (P<0.001), adiponectin (P<0.001), apolipoprotein B (P=0.013), apolipoprotein CIII (P=0.035), lower vascular endothelial growth factor-A (P<0.001), and hepatocyte growth factor (P<0.001) than the control group. Subjects with PAD have greater endothelial apoptosis and oxidative stress than control subjects with low burden of comorbid conditions and cardiovascular risk factors. Furthermore, subjects with PAD have lower systemic antioxidant capacity and angiogenic measures and higher circulating inflammatory parameters. |
| format | Article |
| id | doaj-art-b9da70cd732b46fcb96f4e18f996c249 |
| institution | OA Journals |
| issn | 2090-2824 2090-2832 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Vascular Medicine |
| spelling | doaj-art-b9da70cd732b46fcb96f4e18f996c2492025-08-20T02:18:57ZengWileyInternational Journal of Vascular Medicine2090-28242090-28322014-01-01201410.1155/2014/160534160534Greater Endothelial Apoptosis and Oxidative Stress in Patients with Peripheral Artery DiseaseAndrew W. Gardner0Donald E. Parker1Polly S. Montgomery2Danuta Sosnowska3Ana I. Casanegra4Zoltan Ungvari5Anna Csiszar6William E. Sonntag7Reynolds Oklahoma Center on Aging, Donald W. Reynolds Department of Geriatric Medicine, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK 73117, USADepartment of Biostatistics and Epidemiology, OUHSC, Oklahoma City, OK 73117, USAReynolds Oklahoma Center on Aging, Donald W. Reynolds Department of Geriatric Medicine, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK 73117, USAReynolds Oklahoma Center on Aging, Donald W. Reynolds Department of Geriatric Medicine, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK 73117, USACardiovascular Section, Department of Medicine, OUHSC, Oklahoma City, OK 73117, USAReynolds Oklahoma Center on Aging, Donald W. Reynolds Department of Geriatric Medicine, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK 73117, USAReynolds Oklahoma Center on Aging, Donald W. Reynolds Department of Geriatric Medicine, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK 73117, USAReynolds Oklahoma Center on Aging, Donald W. Reynolds Department of Geriatric Medicine, University of Oklahoma Health Sciences Center (OUHSC), Oklahoma City, OK 73117, USAWe compared apoptosis, cellular oxidative stress, and inflammation of cultured endothelial cells treated with sera from 156 subjects with peripheral artery disease (PAD) and 16 healthy control subjects. Furthermore, we compared circulating inflammatory, antioxidant capacity, and vascular biomarkers between the two groups. The PAD group had a 164% higher value for endothelial cell apoptosis (P<0.001) and a 62% higher value for endothelial cellular reactive oxygen species production (P<0.001) than the control group. Furthermore, the PAD group had lower systemic antioxidant capacity measured by hydroxyl radical antioxidant capacity activity (P<0.001), higher inflammatory and vascular measures of high-sensitivity C-reactive protein (P<0.001), interleukin-8 (P<0.001), serum amyloid A (P<0.001), vascular cell adhesion molecule-1 (P<0.001), adiponectin (P<0.001), apolipoprotein B (P=0.013), apolipoprotein CIII (P=0.035), lower vascular endothelial growth factor-A (P<0.001), and hepatocyte growth factor (P<0.001) than the control group. Subjects with PAD have greater endothelial apoptosis and oxidative stress than control subjects with low burden of comorbid conditions and cardiovascular risk factors. Furthermore, subjects with PAD have lower systemic antioxidant capacity and angiogenic measures and higher circulating inflammatory parameters.http://dx.doi.org/10.1155/2014/160534 |
| spellingShingle | Andrew W. Gardner Donald E. Parker Polly S. Montgomery Danuta Sosnowska Ana I. Casanegra Zoltan Ungvari Anna Csiszar William E. Sonntag Greater Endothelial Apoptosis and Oxidative Stress in Patients with Peripheral Artery Disease International Journal of Vascular Medicine |
| title | Greater Endothelial Apoptosis and Oxidative Stress in Patients with Peripheral Artery Disease |
| title_full | Greater Endothelial Apoptosis and Oxidative Stress in Patients with Peripheral Artery Disease |
| title_fullStr | Greater Endothelial Apoptosis and Oxidative Stress in Patients with Peripheral Artery Disease |
| title_full_unstemmed | Greater Endothelial Apoptosis and Oxidative Stress in Patients with Peripheral Artery Disease |
| title_short | Greater Endothelial Apoptosis and Oxidative Stress in Patients with Peripheral Artery Disease |
| title_sort | greater endothelial apoptosis and oxidative stress in patients with peripheral artery disease |
| url | http://dx.doi.org/10.1155/2014/160534 |
| work_keys_str_mv | AT andrewwgardner greaterendothelialapoptosisandoxidativestressinpatientswithperipheralarterydisease AT donaldeparker greaterendothelialapoptosisandoxidativestressinpatientswithperipheralarterydisease AT pollysmontgomery greaterendothelialapoptosisandoxidativestressinpatientswithperipheralarterydisease AT danutasosnowska greaterendothelialapoptosisandoxidativestressinpatientswithperipheralarterydisease AT anaicasanegra greaterendothelialapoptosisandoxidativestressinpatientswithperipheralarterydisease AT zoltanungvari greaterendothelialapoptosisandoxidativestressinpatientswithperipheralarterydisease AT annacsiszar greaterendothelialapoptosisandoxidativestressinpatientswithperipheralarterydisease AT williamesonntag greaterendothelialapoptosisandoxidativestressinpatientswithperipheralarterydisease |