Common Variable Immunodeficiency and Circulating TFH
CD4+ T follicular helper cells (TFH) were assessed in adult patients with common variable immune deficiency (CVID) classified according to the presence of granulomatous disease (GD), autoimmunity (AI), or both GD and AI (Group I) or the absence of AI and GD (Group II). TFH lymphocytes were character...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4951587 |
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| author | Ana Coraglia Nora Galassi Diego S. Fernández Romero M. Cecilia Juri Marta Felippo Alejandro Malbrán María M. E. de Bracco |
| author_facet | Ana Coraglia Nora Galassi Diego S. Fernández Romero M. Cecilia Juri Marta Felippo Alejandro Malbrán María M. E. de Bracco |
| author_sort | Ana Coraglia |
| collection | DOAJ |
| description | CD4+ T follicular helper cells (TFH) were assessed in adult patients with common variable immune deficiency (CVID) classified according to the presence of granulomatous disease (GD), autoimmunity (AI), or both GD and AI (Group I) or the absence of AI and GD (Group II). TFH lymphocytes were characterized by expression of CXCR5 and PD-1. TFH were higher (in both absolute number and percentage) in Group I than in Group II CVID patients and normal controls (N). Within CXCR5+CD4+ T cells, the percentage of PD-1 (+) was higher and that of CCR7 (+) was lower in Group I than in Group II and N. The percentages of Treg and TFH reg were similar in both CVID groups and in N. TFH responded to stimulation increasing the expression of the costimulatory molecules CD40L and ICOS as did N. After submitogenic PHA+IL-2 stimulation, intracellular expression of TFH cytokines (IL-10, IL-21) was higher than N in Group I, and IL-4 was higher than N in Group II. These results suggest that TFH are functional in CVID and highlight the association of increased circulating TFH with AI and GD manifestations. |
| format | Article |
| id | doaj-art-b9da62f1bdca488e9460c5e60964c135 |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-b9da62f1bdca488e9460c5e60964c1352025-08-20T02:18:57ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/49515874951587Common Variable Immunodeficiency and Circulating TFHAna Coraglia0Nora Galassi1Diego S. Fernández Romero2M. Cecilia Juri3Marta Felippo4Alejandro Malbrán5María M. E. de Bracco6Instituto de Investigaciones Hematológicas (IIHEMA), Academia Nacional de Medicina, C1425ASU Ciudad Autónoma de Buenos Aires, ArgentinaIMEX-CONICET, Academia Nacional de Medicina, C1425ASU Ciudad Autónoma de Buenos Aires, ArgentinaUnidad de Alergia, Asma e Inmunología Clínica, C1035AAT Ciudad Autónoma de Buenos Aires, ArgentinaUnidad de Alergia, Asma e Inmunología Clínica, C1035AAT Ciudad Autónoma de Buenos Aires, ArgentinaIMEX-CONICET, Academia Nacional de Medicina, C1425ASU Ciudad Autónoma de Buenos Aires, ArgentinaUnidad de Alergia, Asma e Inmunología Clínica, C1035AAT Ciudad Autónoma de Buenos Aires, ArgentinaInstituto de Investigaciones Hematológicas (IIHEMA), Academia Nacional de Medicina, C1425ASU Ciudad Autónoma de Buenos Aires, ArgentinaCD4+ T follicular helper cells (TFH) were assessed in adult patients with common variable immune deficiency (CVID) classified according to the presence of granulomatous disease (GD), autoimmunity (AI), or both GD and AI (Group I) or the absence of AI and GD (Group II). TFH lymphocytes were characterized by expression of CXCR5 and PD-1. TFH were higher (in both absolute number and percentage) in Group I than in Group II CVID patients and normal controls (N). Within CXCR5+CD4+ T cells, the percentage of PD-1 (+) was higher and that of CCR7 (+) was lower in Group I than in Group II and N. The percentages of Treg and TFH reg were similar in both CVID groups and in N. TFH responded to stimulation increasing the expression of the costimulatory molecules CD40L and ICOS as did N. After submitogenic PHA+IL-2 stimulation, intracellular expression of TFH cytokines (IL-10, IL-21) was higher than N in Group I, and IL-4 was higher than N in Group II. These results suggest that TFH are functional in CVID and highlight the association of increased circulating TFH with AI and GD manifestations.http://dx.doi.org/10.1155/2016/4951587 |
| spellingShingle | Ana Coraglia Nora Galassi Diego S. Fernández Romero M. Cecilia Juri Marta Felippo Alejandro Malbrán María M. E. de Bracco Common Variable Immunodeficiency and Circulating TFH Journal of Immunology Research |
| title | Common Variable Immunodeficiency and Circulating TFH |
| title_full | Common Variable Immunodeficiency and Circulating TFH |
| title_fullStr | Common Variable Immunodeficiency and Circulating TFH |
| title_full_unstemmed | Common Variable Immunodeficiency and Circulating TFH |
| title_short | Common Variable Immunodeficiency and Circulating TFH |
| title_sort | common variable immunodeficiency and circulating tfh |
| url | http://dx.doi.org/10.1155/2016/4951587 |
| work_keys_str_mv | AT anacoraglia commonvariableimmunodeficiencyandcirculatingtfh AT noragalassi commonvariableimmunodeficiencyandcirculatingtfh AT diegosfernandezromero commonvariableimmunodeficiencyandcirculatingtfh AT mceciliajuri commonvariableimmunodeficiencyandcirculatingtfh AT martafelippo commonvariableimmunodeficiencyandcirculatingtfh AT alejandromalbran commonvariableimmunodeficiencyandcirculatingtfh AT mariamedebracco commonvariableimmunodeficiencyandcirculatingtfh |