The PARP inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse models

Abstract Reovirus type 3 Dearing (RT3D) is an oncolytic, double-stranded RNA virus. To identify potential RT3D drug-viral sensitizer, here we use a high-throughput screen of therapeutic agents and find a PARP-1 inhibitor, talazoparib, as a top hit. RT3D interacts with retinoic acid-induced gene-1 (R...

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Main Authors: Joan Kyula-Currie, Victoria Roulstone, James Wright, Francesca Butera, Arnaud Legrand, Richard Elliott, Martin McLaughlin, Galabina Bozhanova, Dragomir Krastev, Stephen Pettitt, Tencho Tenev, Magnus Dillon, Shane Foo, Emmanuel C. Patin, Victoria Jennings, Charleen Chan Wah Hak, Elizabeth Appleton, Amarin Wongariyapak, Malin Pedersen, Antonio Rullan, Jyoti Choudhary, Chris Bakal, Pascal Meier, Christopher J. Lord, Alan Melcher, Kevin J. Harrington
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61297-w
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author Joan Kyula-Currie
Victoria Roulstone
James Wright
Francesca Butera
Arnaud Legrand
Richard Elliott
Martin McLaughlin
Galabina Bozhanova
Dragomir Krastev
Stephen Pettitt
Tencho Tenev
Magnus Dillon
Shane Foo
Emmanuel C. Patin
Victoria Jennings
Charleen Chan Wah Hak
Elizabeth Appleton
Amarin Wongariyapak
Malin Pedersen
Antonio Rullan
Jyoti Choudhary
Chris Bakal
Pascal Meier
Christopher J. Lord
Alan Melcher
Kevin J. Harrington
author_facet Joan Kyula-Currie
Victoria Roulstone
James Wright
Francesca Butera
Arnaud Legrand
Richard Elliott
Martin McLaughlin
Galabina Bozhanova
Dragomir Krastev
Stephen Pettitt
Tencho Tenev
Magnus Dillon
Shane Foo
Emmanuel C. Patin
Victoria Jennings
Charleen Chan Wah Hak
Elizabeth Appleton
Amarin Wongariyapak
Malin Pedersen
Antonio Rullan
Jyoti Choudhary
Chris Bakal
Pascal Meier
Christopher J. Lord
Alan Melcher
Kevin J. Harrington
author_sort Joan Kyula-Currie
collection DOAJ
description Abstract Reovirus type 3 Dearing (RT3D) is an oncolytic, double-stranded RNA virus. To identify potential RT3D drug-viral sensitizer, here we use a high-throughput screen of therapeutic agents and find a PARP-1 inhibitor, talazoparib, as a top hit. RT3D interacts with retinoic acid-induced gene-1 (RIG-I) and activates PARP-1, with consequent PARylation of components of the extrinsic apoptosis pathway. Pharmacological or genetic inhibition of PARP-1 abrogates this PARylation and enhances extrinsic apoptosis, NF-kB signalling and pro-inflammatory cell death. Interaction between PARP-1 and RIG-I induced by treating RT3D-infected cells with talazoparib activates downstream IFN-β and TNF/TRAIL production to amplify the therapeutic effect through positive feedback. Furthermore, the effect of RT3D-talazoparib combination is phenocopied by non-viral ds-RNA therapy and RIG-I agonism. In vivo, mouse tumour model results show that RT3D/talazoparib combination regimen induces complete control of inoculated tumour as well as protection from subsequent tumour rechallenge with the, with accompanied innate and adaptive immune activation.
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spelling doaj-art-b9d6e4a2bc7545d692bb585d15d6a84e2025-08-20T03:05:06ZengNature PortfolioNature Communications2041-17232025-07-0116111910.1038/s41467-025-61297-wThe PARP inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse modelsJoan Kyula-Currie0Victoria Roulstone1James Wright2Francesca Butera3Arnaud Legrand4Richard Elliott5Martin McLaughlin6Galabina Bozhanova7Dragomir Krastev8Stephen Pettitt9Tencho Tenev10Magnus Dillon11Shane Foo12Emmanuel C. Patin13Victoria Jennings14Charleen Chan Wah Hak15Elizabeth Appleton16Amarin Wongariyapak17Malin Pedersen18Antonio Rullan19Jyoti Choudhary20Chris Bakal21Pascal Meier22Christopher J. Lord23Alan Melcher24Kevin J. Harrington25The Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe CRUK Gene Function Laboratory and Breast Cancer Now Toby Robins ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe CRUK Gene Function Laboratory and Breast Cancer Now Toby Robins ResearchThe CRUK Gene Function Laboratory and Breast Cancer Now Toby Robins ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchThe CRUK Gene Function Laboratory and Breast Cancer Now Toby Robins ResearchThe Institute of Cancer ResearchThe Institute of Cancer ResearchAbstract Reovirus type 3 Dearing (RT3D) is an oncolytic, double-stranded RNA virus. To identify potential RT3D drug-viral sensitizer, here we use a high-throughput screen of therapeutic agents and find a PARP-1 inhibitor, talazoparib, as a top hit. RT3D interacts with retinoic acid-induced gene-1 (RIG-I) and activates PARP-1, with consequent PARylation of components of the extrinsic apoptosis pathway. Pharmacological or genetic inhibition of PARP-1 abrogates this PARylation and enhances extrinsic apoptosis, NF-kB signalling and pro-inflammatory cell death. Interaction between PARP-1 and RIG-I induced by treating RT3D-infected cells with talazoparib activates downstream IFN-β and TNF/TRAIL production to amplify the therapeutic effect through positive feedback. Furthermore, the effect of RT3D-talazoparib combination is phenocopied by non-viral ds-RNA therapy and RIG-I agonism. In vivo, mouse tumour model results show that RT3D/talazoparib combination regimen induces complete control of inoculated tumour as well as protection from subsequent tumour rechallenge with the, with accompanied innate and adaptive immune activation.https://doi.org/10.1038/s41467-025-61297-w
spellingShingle Joan Kyula-Currie
Victoria Roulstone
James Wright
Francesca Butera
Arnaud Legrand
Richard Elliott
Martin McLaughlin
Galabina Bozhanova
Dragomir Krastev
Stephen Pettitt
Tencho Tenev
Magnus Dillon
Shane Foo
Emmanuel C. Patin
Victoria Jennings
Charleen Chan Wah Hak
Elizabeth Appleton
Amarin Wongariyapak
Malin Pedersen
Antonio Rullan
Jyoti Choudhary
Chris Bakal
Pascal Meier
Christopher J. Lord
Alan Melcher
Kevin J. Harrington
The PARP inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse models
Nature Communications
title The PARP inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse models
title_full The PARP inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse models
title_fullStr The PARP inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse models
title_full_unstemmed The PARP inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse models
title_short The PARP inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse models
title_sort parp inhibitor talazoparib synergizes with reovirus to induce cancer killing and tumour control in vivo in mouse models
url https://doi.org/10.1038/s41467-025-61297-w
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