Cultivation of Entamoeba histolytica in vitro and diagnose the bacterial growths in culture media
The parasite was isolated from a stool sample, cultivated and maintained in vitro using Locke-egg medium (LEM) and Liver infusion agar medium (LIAM) . The culture was maintained for up to 21 months, and the best time to maintain the parasite was every 48 hours, although the growth in the culture me...
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| Format: | Article |
| Language: | English |
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University of Baghdad, College of Science for Women
2009-09-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1001 |
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| author | Baghdad Science Journal |
| author_facet | Baghdad Science Journal |
| author_sort | Baghdad Science Journal |
| collection | DOAJ |
| description | The parasite was isolated from a stool sample, cultivated and maintained in vitro using Locke-egg medium (LEM) and Liver infusion agar medium (LIAM) . The culture was maintained for up to 21 months, and the best time to maintain the parasite was every 48 hours, although the growth in the culture media continued for 13 days without a maintenance. Additionally, no cyst formation was observed during cultivation of parasite in the two culture media. Although, was observe young cyst formed in LEM media were deletion of maintained. The diagnosis of bacteria growth in the culture media, bacterial content (Escherichia coli) was an dominance and essential requirement for a successful cultivation of Entamoeba histolytica in the two culture media. |
| format | Article |
| id | doaj-art-b9d2e993e6d146e8ba0d182d86f64c85 |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2009-09-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-b9d2e993e6d146e8ba0d182d86f64c852025-08-20T03:39:26ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862009-09-016310.21123/bsj.6.3.442-447Cultivation of Entamoeba histolytica in vitro and diagnose the bacterial growths in culture mediaBaghdad Science JournalThe parasite was isolated from a stool sample, cultivated and maintained in vitro using Locke-egg medium (LEM) and Liver infusion agar medium (LIAM) . The culture was maintained for up to 21 months, and the best time to maintain the parasite was every 48 hours, although the growth in the culture media continued for 13 days without a maintenance. Additionally, no cyst formation was observed during cultivation of parasite in the two culture media. Although, was observe young cyst formed in LEM media were deletion of maintained. The diagnosis of bacteria growth in the culture media, bacterial content (Escherichia coli) was an dominance and essential requirement for a successful cultivation of Entamoeba histolytica in the two culture media.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1001"Cultivation , Entamoeba histolytica , Escherichia coli" |
| spellingShingle | Baghdad Science Journal Cultivation of Entamoeba histolytica in vitro and diagnose the bacterial growths in culture media مجلة بغداد للعلوم "Cultivation , Entamoeba histolytica , Escherichia coli" |
| title | Cultivation of Entamoeba histolytica in vitro and diagnose the bacterial growths in culture media |
| title_full | Cultivation of Entamoeba histolytica in vitro and diagnose the bacterial growths in culture media |
| title_fullStr | Cultivation of Entamoeba histolytica in vitro and diagnose the bacterial growths in culture media |
| title_full_unstemmed | Cultivation of Entamoeba histolytica in vitro and diagnose the bacterial growths in culture media |
| title_short | Cultivation of Entamoeba histolytica in vitro and diagnose the bacterial growths in culture media |
| title_sort | cultivation of entamoeba histolytica in vitro and diagnose the bacterial growths in culture media |
| topic | "Cultivation , Entamoeba histolytica , Escherichia coli" |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1001 |
| work_keys_str_mv | AT baghdadsciencejournal cultivationofentamoebahistolyticainvitroanddiagnosethebacterialgrowthsinculturemedia |