Development and studies on LEAFY genetic transformation system with high efficiency in commercial grape (Vitis vinifera L. var muscat)
Transgenic plants appear to be a new way to grape improvement and a powerful tool for studying gene functions. Using a direct high frequency shoot regeneration system suitable for genetic transformation was established successfully in this study, which was the base for the following research of cons...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2001-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2001.05.0523 |
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| author | CHEN Li-geng LIU Shu-fang HU Xi-qin |
| author_facet | CHEN Li-geng LIU Shu-fang HU Xi-qin |
| author_sort | CHEN Li-geng |
| collection | DOAJ |
| description | Transgenic plants appear to be a new way to grape improvement and a powerful tool for studying gene functions. Using a direct high frequency shoot regeneration system suitable for genetic transformation was established successfully in this study, which was the base for the following research of construction of a direct high frequency shoot regeneration system suitable for genetic transformation was established successfully by using pieces of fresh stem of Muscat of Alexandria (Vitis vinifera L. var. muscat) as explants, and transformed plants were obtained with a higher transformation efficiency. Stem pieces from in vitro-grow plants were cocultivated for 3 days with Agrobacterium tumefaciens strain GV3101 containing the vector pDC202 in which LEAFY gene from Arabrodopsis has been inserted. It was followed by incubating on regeneration medium (GS+ZT 2.0 mg·L<sup>-1</sup>) containing rif, and gen for 4 weeks, After observed according its growth status and identified by use of PCR and Southern blot, the transformed plants was obtained with roots growing at first and the buds at second, which was different from some other trarisgenic plants to some extent, and the regeneration percentage of transformed buds was 6.8%. |
| format | Article |
| id | doaj-art-b9cf506624a94b4caac9ac1d87bf1775 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2001-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-b9cf506624a94b4caac9ac1d87bf17752025-08-20T02:52:51ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552001-09-012752352610.3785/1008-9209.2001.05.052310089209Development and studies on LEAFY genetic transformation system with high efficiency in commercial grape (Vitis vinifera L. var muscat)CHEN Li-gengLIU Shu-fangHU Xi-qinTransgenic plants appear to be a new way to grape improvement and a powerful tool for studying gene functions. Using a direct high frequency shoot regeneration system suitable for genetic transformation was established successfully in this study, which was the base for the following research of construction of a direct high frequency shoot regeneration system suitable for genetic transformation was established successfully by using pieces of fresh stem of Muscat of Alexandria (Vitis vinifera L. var. muscat) as explants, and transformed plants were obtained with a higher transformation efficiency. Stem pieces from in vitro-grow plants were cocultivated for 3 days with Agrobacterium tumefaciens strain GV3101 containing the vector pDC202 in which LEAFY gene from Arabrodopsis has been inserted. It was followed by incubating on regeneration medium (GS+ZT 2.0 mg·L<sup>-1</sup>) containing rif, and gen for 4 weeks, After observed according its growth status and identified by use of PCR and Southern blot, the transformed plants was obtained with roots growing at first and the buds at second, which was different from some other trarisgenic plants to some extent, and the regeneration percentage of transformed buds was 6.8%.https://www.academax.com/doi/10.3785/1008-9209.2001.05.0523grapevine<italic>LEAFY</italic> genetransformation efficiency |
| spellingShingle | CHEN Li-geng LIU Shu-fang HU Xi-qin Development and studies on LEAFY genetic transformation system with high efficiency in commercial grape (Vitis vinifera L. var muscat) 浙江大学学报. 农业与生命科学版 grapevine <italic>LEAFY</italic> gene transformation efficiency |
| title | Development and studies on LEAFY genetic transformation system with high efficiency in commercial grape (Vitis vinifera L. var muscat) |
| title_full | Development and studies on LEAFY genetic transformation system with high efficiency in commercial grape (Vitis vinifera L. var muscat) |
| title_fullStr | Development and studies on LEAFY genetic transformation system with high efficiency in commercial grape (Vitis vinifera L. var muscat) |
| title_full_unstemmed | Development and studies on LEAFY genetic transformation system with high efficiency in commercial grape (Vitis vinifera L. var muscat) |
| title_short | Development and studies on LEAFY genetic transformation system with high efficiency in commercial grape (Vitis vinifera L. var muscat) |
| title_sort | development and studies on leafy genetic transformation system with high efficiency in commercial grape vitis vinifera l var muscat |
| topic | grapevine <italic>LEAFY</italic> gene transformation efficiency |
| url | https://www.academax.com/doi/10.3785/1008-9209.2001.05.0523 |
| work_keys_str_mv | AT chenligeng developmentandstudiesonleafygenetictransformationsystemwithhighefficiencyincommercialgrapevitisviniferalvarmuscat AT liushufang developmentandstudiesonleafygenetictransformationsystemwithhighefficiencyincommercialgrapevitisviniferalvarmuscat AT huxiqin developmentandstudiesonleafygenetictransformationsystemwithhighefficiencyincommercialgrapevitisviniferalvarmuscat |