Cytotoxic effects of five Iranian medicinal plants on pancreatic cancer cell lines and investigation of induced changes in the expression of several key genes
Abstract Cancer is a deadly and fast-spreading disease that is a growing health problem worldwide due to a lack of comprehensive screening and appropriate medication. However, natural products derived from medicinal plants have gained attention as potential sources of bioactive compounds that select...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-025-04970-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849238108881551360 |
|---|---|
| author | Sahar Akrami Shirin Omidvar Kordshouli Ahmad Tahmasebi Ali Moghadam Farzaneh Aram Ali Golestan Amin Ramezani Ali Niazi |
| author_facet | Sahar Akrami Shirin Omidvar Kordshouli Ahmad Tahmasebi Ali Moghadam Farzaneh Aram Ali Golestan Amin Ramezani Ali Niazi |
| author_sort | Sahar Akrami |
| collection | DOAJ |
| description | Abstract Cancer is a deadly and fast-spreading disease that is a growing health problem worldwide due to a lack of comprehensive screening and appropriate medication. However, natural products derived from medicinal plants have gained attention as potential sources of bioactive compounds that selectively remove cancerous lesions and are nontoxic and safe. Pancreatic cancer (PC) is a major therapeutic challenge and is predicted to surpass breast cancer as the third leading cause of cancer death. This study investigated the cytotoxic effects of methanolic extracts from five Iranian medicinal plants, Cuscuta epithymum, Achillea millefolium, Salvia officinalis, Salvia hydrangea, and Teucrium polium, on pancreatic cancer cell lines (MIA PaCa-2 and PaTu8902). Additionally, we examined the changes in the expression of key genes following treatment with C. epithymum extract. The findings revealed that the plant extracts had a dose-dependent effect on the cell viability of the lines, with the C. epithymum extract exhibiting the greatest cytotoxic effect (IC50 values of 85.03 µg/mL for MIA PaCa-2 and 156.57 µg/mL for PaTu 8902). GC‒MS analysis revealed 25 bioactive compounds in C. epithymum, with quinic acid (14.13%), p-vinylphenol (13.22%), and valeraldehyde (11.21%) as the most abundant. The study also investigated the changes in the expression of the STAT4, PIK3CD, EMP1, and RAB11FIP3 genes in MIA PaCa-2 and PaTu 8902 pancreatic cancer cell lines after treatment with the extract from C. epithymum. A correlation was detected between the expression levels of PIK3CD, STAT4, EMP1, and RAB11FIP3 and various concentrations of C. epithymum extract. The results revealed that the extract increased the mRNA levels of STAT4, PIK3CD, and EMP1, whereas RAB11FIP3 was reduced in the treated cells. Accordingly, C. epithymum extract has strong cytotoxic effects on pancreatic cancer cells and influences the expression of key cancer-related genes, suggesting its potential as a therapeutic candidate for PC treatment. Further in vivo studies are recommended to explore its mechanisms of action and clinical applicability. Graphical Abstract |
| format | Article |
| id | doaj-art-b9cd2de2c79a4147bfdfcb0a1ee6f8b6 |
| institution | Kabale University |
| issn | 2662-7671 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj-art-b9cd2de2c79a4147bfdfcb0a1ee6f8b62025-08-20T04:01:46ZengBMCBMC Complementary Medicine and Therapies2662-76712025-07-0125111210.1186/s12906-025-04970-3Cytotoxic effects of five Iranian medicinal plants on pancreatic cancer cell lines and investigation of induced changes in the expression of several key genesSahar Akrami0Shirin Omidvar Kordshouli1Ahmad Tahmasebi2Ali Moghadam3Farzaneh Aram4Ali Golestan5Amin Ramezani6Ali Niazi7Institute of Biotechnology, Shiraz UniversityInstitute of Biotechnology, Shiraz UniversityInstitute of Biotechnology, Shiraz UniversityInstitute of Biotechnology, Shiraz UniversityInstitute of Biotechnology, Shiraz UniversityDepartment of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical SciencesDepartment of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical SciencesInstitute of Biotechnology, Shiraz UniversityAbstract Cancer is a deadly and fast-spreading disease that is a growing health problem worldwide due to a lack of comprehensive screening and appropriate medication. However, natural products derived from medicinal plants have gained attention as potential sources of bioactive compounds that selectively remove cancerous lesions and are nontoxic and safe. Pancreatic cancer (PC) is a major therapeutic challenge and is predicted to surpass breast cancer as the third leading cause of cancer death. This study investigated the cytotoxic effects of methanolic extracts from five Iranian medicinal plants, Cuscuta epithymum, Achillea millefolium, Salvia officinalis, Salvia hydrangea, and Teucrium polium, on pancreatic cancer cell lines (MIA PaCa-2 and PaTu8902). Additionally, we examined the changes in the expression of key genes following treatment with C. epithymum extract. The findings revealed that the plant extracts had a dose-dependent effect on the cell viability of the lines, with the C. epithymum extract exhibiting the greatest cytotoxic effect (IC50 values of 85.03 µg/mL for MIA PaCa-2 and 156.57 µg/mL for PaTu 8902). GC‒MS analysis revealed 25 bioactive compounds in C. epithymum, with quinic acid (14.13%), p-vinylphenol (13.22%), and valeraldehyde (11.21%) as the most abundant. The study also investigated the changes in the expression of the STAT4, PIK3CD, EMP1, and RAB11FIP3 genes in MIA PaCa-2 and PaTu 8902 pancreatic cancer cell lines after treatment with the extract from C. epithymum. A correlation was detected between the expression levels of PIK3CD, STAT4, EMP1, and RAB11FIP3 and various concentrations of C. epithymum extract. The results revealed that the extract increased the mRNA levels of STAT4, PIK3CD, and EMP1, whereas RAB11FIP3 was reduced in the treated cells. Accordingly, C. epithymum extract has strong cytotoxic effects on pancreatic cancer cells and influences the expression of key cancer-related genes, suggesting its potential as a therapeutic candidate for PC treatment. Further in vivo studies are recommended to explore its mechanisms of action and clinical applicability. Graphical Abstracthttps://doi.org/10.1186/s12906-025-04970-3Pancreatic cancerMedicinal plantGC–MS analysisGene expression |
| spellingShingle | Sahar Akrami Shirin Omidvar Kordshouli Ahmad Tahmasebi Ali Moghadam Farzaneh Aram Ali Golestan Amin Ramezani Ali Niazi Cytotoxic effects of five Iranian medicinal plants on pancreatic cancer cell lines and investigation of induced changes in the expression of several key genes BMC Complementary Medicine and Therapies Pancreatic cancer Medicinal plant GC–MS analysis Gene expression |
| title | Cytotoxic effects of five Iranian medicinal plants on pancreatic cancer cell lines and investigation of induced changes in the expression of several key genes |
| title_full | Cytotoxic effects of five Iranian medicinal plants on pancreatic cancer cell lines and investigation of induced changes in the expression of several key genes |
| title_fullStr | Cytotoxic effects of five Iranian medicinal plants on pancreatic cancer cell lines and investigation of induced changes in the expression of several key genes |
| title_full_unstemmed | Cytotoxic effects of five Iranian medicinal plants on pancreatic cancer cell lines and investigation of induced changes in the expression of several key genes |
| title_short | Cytotoxic effects of five Iranian medicinal plants on pancreatic cancer cell lines and investigation of induced changes in the expression of several key genes |
| title_sort | cytotoxic effects of five iranian medicinal plants on pancreatic cancer cell lines and investigation of induced changes in the expression of several key genes |
| topic | Pancreatic cancer Medicinal plant GC–MS analysis Gene expression |
| url | https://doi.org/10.1186/s12906-025-04970-3 |
| work_keys_str_mv | AT saharakrami cytotoxiceffectsoffiveiranianmedicinalplantsonpancreaticcancercelllinesandinvestigationofinducedchangesintheexpressionofseveralkeygenes AT shirinomidvarkordshouli cytotoxiceffectsoffiveiranianmedicinalplantsonpancreaticcancercelllinesandinvestigationofinducedchangesintheexpressionofseveralkeygenes AT ahmadtahmasebi cytotoxiceffectsoffiveiranianmedicinalplantsonpancreaticcancercelllinesandinvestigationofinducedchangesintheexpressionofseveralkeygenes AT alimoghadam cytotoxiceffectsoffiveiranianmedicinalplantsonpancreaticcancercelllinesandinvestigationofinducedchangesintheexpressionofseveralkeygenes AT farzaneharam cytotoxiceffectsoffiveiranianmedicinalplantsonpancreaticcancercelllinesandinvestigationofinducedchangesintheexpressionofseveralkeygenes AT aligolestan cytotoxiceffectsoffiveiranianmedicinalplantsonpancreaticcancercelllinesandinvestigationofinducedchangesintheexpressionofseveralkeygenes AT aminramezani cytotoxiceffectsoffiveiranianmedicinalplantsonpancreaticcancercelllinesandinvestigationofinducedchangesintheexpressionofseveralkeygenes AT aliniazi cytotoxiceffectsoffiveiranianmedicinalplantsonpancreaticcancercelllinesandinvestigationofinducedchangesintheexpressionofseveralkeygenes |