Muscle fibre satellite cells are located at a greater distance from capillaries in patients with COPD compared with healthy controls
Background COPD is a disease characterised by skeletal muscle dysfunction. A spatial relationship exists between satellite cells and muscle fibre capillaries, which has been suggested to be of major importance for satellite cell function. In the present study we compared the spatial relationship bet...
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| Format: | Article |
| Language: | English |
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European Respiratory Society
2024-09-01
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| Series: | ERJ Open Research |
| Online Access: | http://openres.ersjournals.com/content/10/5/00203-2024.full |
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| author | Milan W. Betz Jana De Brandt Thorben Aussieker Alejandra P. Monsegue Dion C.J. Houtvast Sebastian Gehlert Lex B. Verdijk Luc J.C. van Loon Harry R. Gosker Ramon J.C. Langen Wim Derave Chris Burtin Martijn A. Spruit Tim Snijders |
| author_facet | Milan W. Betz Jana De Brandt Thorben Aussieker Alejandra P. Monsegue Dion C.J. Houtvast Sebastian Gehlert Lex B. Verdijk Luc J.C. van Loon Harry R. Gosker Ramon J.C. Langen Wim Derave Chris Burtin Martijn A. Spruit Tim Snijders |
| author_sort | Milan W. Betz |
| collection | DOAJ |
| description | Background
COPD is a disease characterised by skeletal muscle dysfunction. A spatial relationship exists between satellite cells and muscle fibre capillaries, which has been suggested to be of major importance for satellite cell function. In the present study we compared the spatial relationship between satellite cells and capillaries in patients with COPD and age-matched healthy older adults.
Methods
Muscle biopsies were obtained from the vastus lateralis of n=18 patients with COPD (8 female, 10 male; age 66±5 years, mild-to-severe airflow obstruction) and n=18 age-, sex- and body mass index-matched healthy control adults (8 female, 10 male; age 68±5 years). Immunohistochemistry was used to assess type I/II muscle fibre size, distribution, myonuclear content, satellite cell number and fibre capillarisation. In addition, type I/II muscle fibre satellite cell distance to its nearest capillary was assessed.
Results
The percentage of type II muscle fibres was significantly greater in patients with COPD (62±10%) compared with controls (50±12%, p<0.05). Muscle fibre capillarisation was significantly lower in patients with COPD compared with controls (p<0.05). While satellite cell content was not different between groups, type I and type II satellite cell distance to its nearest capillary was significantly greater in patients with COPD (type I: 21.3±4.8 µm; type II: 26.7±9.3 µm) compared with controls (type I: 16.1±3.5 µm; type II: 22.7±5.8 µm; p<0.05).
Conclusion
Satellite cells are located at a greater distance from their nearest capillary in patients with COPD compared with age-matched controls. This increased distance could play a role in impaired satellite cell function in patients with COPD. |
| format | Article |
| id | doaj-art-b9c377367a094f199bfaa5d298380a68 |
| institution | OA Journals |
| issn | 2312-0541 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | European Respiratory Society |
| record_format | Article |
| series | ERJ Open Research |
| spelling | doaj-art-b9c377367a094f199bfaa5d298380a682025-08-20T02:14:11ZengEuropean Respiratory SocietyERJ Open Research2312-05412024-09-0110510.1183/23120541.00203-202400203-2024Muscle fibre satellite cells are located at a greater distance from capillaries in patients with COPD compared with healthy controlsMilan W. Betz0Jana De Brandt1Thorben Aussieker2Alejandra P. Monsegue3Dion C.J. Houtvast4Sebastian Gehlert5Lex B. Verdijk6Luc J.C. van Loon7Harry R. Gosker8Ramon J.C. Langen9Wim Derave10Chris Burtin11Martijn A. Spruit12Tim Snijders13 NUTRIM Research Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Department of Human Biology, Maastricht, The Netherlands Faculty of Rehabilitation Sciences, REVAL – Rehabilitation Research Center, BIOMED – Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium NUTRIM Research Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Department of Human Biology, Maastricht, The Netherlands NUTRIM Research Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Department of Human Biology, Maastricht, The Netherlands NUTRIM Research Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Department of Human Biology, Maastricht, The Netherlands Department for Biosciences of Sports, Institute for Sport Science, University of Hildesheim, Hildesheim, Germany NUTRIM Research Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Department of Human Biology, Maastricht, The Netherlands NUTRIM Research Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Department of Human Biology, Maastricht, The Netherlands NUTRIM, Maastricht University Medical Centre+, Department of Respiratory Medicine, Maastricht, The Netherlands NUTRIM, Maastricht University Medical Centre+, Department of Respiratory Medicine, Maastricht, The Netherlands Department of Movement and Sports Sciences, Ghent University, Ghent, Belgium Faculty of Rehabilitation Sciences, REVAL – Rehabilitation Research Center, BIOMED – Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium NUTRIM, Maastricht University Medical Centre+, Department of Respiratory Medicine, Maastricht, The Netherlands NUTRIM Research Institute of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+, Department of Human Biology, Maastricht, The Netherlands Background COPD is a disease characterised by skeletal muscle dysfunction. A spatial relationship exists between satellite cells and muscle fibre capillaries, which has been suggested to be of major importance for satellite cell function. In the present study we compared the spatial relationship between satellite cells and capillaries in patients with COPD and age-matched healthy older adults. Methods Muscle biopsies were obtained from the vastus lateralis of n=18 patients with COPD (8 female, 10 male; age 66±5 years, mild-to-severe airflow obstruction) and n=18 age-, sex- and body mass index-matched healthy control adults (8 female, 10 male; age 68±5 years). Immunohistochemistry was used to assess type I/II muscle fibre size, distribution, myonuclear content, satellite cell number and fibre capillarisation. In addition, type I/II muscle fibre satellite cell distance to its nearest capillary was assessed. Results The percentage of type II muscle fibres was significantly greater in patients with COPD (62±10%) compared with controls (50±12%, p<0.05). Muscle fibre capillarisation was significantly lower in patients with COPD compared with controls (p<0.05). While satellite cell content was not different between groups, type I and type II satellite cell distance to its nearest capillary was significantly greater in patients with COPD (type I: 21.3±4.8 µm; type II: 26.7±9.3 µm) compared with controls (type I: 16.1±3.5 µm; type II: 22.7±5.8 µm; p<0.05). Conclusion Satellite cells are located at a greater distance from their nearest capillary in patients with COPD compared with age-matched controls. This increased distance could play a role in impaired satellite cell function in patients with COPD.http://openres.ersjournals.com/content/10/5/00203-2024.full |
| spellingShingle | Milan W. Betz Jana De Brandt Thorben Aussieker Alejandra P. Monsegue Dion C.J. Houtvast Sebastian Gehlert Lex B. Verdijk Luc J.C. van Loon Harry R. Gosker Ramon J.C. Langen Wim Derave Chris Burtin Martijn A. Spruit Tim Snijders Muscle fibre satellite cells are located at a greater distance from capillaries in patients with COPD compared with healthy controls ERJ Open Research |
| title | Muscle fibre satellite cells are located at a greater distance from capillaries in patients with COPD compared with healthy controls |
| title_full | Muscle fibre satellite cells are located at a greater distance from capillaries in patients with COPD compared with healthy controls |
| title_fullStr | Muscle fibre satellite cells are located at a greater distance from capillaries in patients with COPD compared with healthy controls |
| title_full_unstemmed | Muscle fibre satellite cells are located at a greater distance from capillaries in patients with COPD compared with healthy controls |
| title_short | Muscle fibre satellite cells are located at a greater distance from capillaries in patients with COPD compared with healthy controls |
| title_sort | muscle fibre satellite cells are located at a greater distance from capillaries in patients with copd compared with healthy controls |
| url | http://openres.ersjournals.com/content/10/5/00203-2024.full |
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