Analysis of association between FGB, MTHFR, MTR and MTRR genes polymorphisms and ischemic and hemorrhagic stroke
The genetic component is a leading factor of stroke manifestation. Contribution of single nucleotide polymorphisms or combined genotypes to disease development has population differences. Special attention should be paid to genetic features in different types of strokes among the Ukrainian populatio...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zaporizhzhia State Medical and Pharmaceutical University
2020-08-01
|
| Series: | Zaporožskij Medicinskij Žurnal |
| Subjects: | |
| Online Access: | http://zmj.zsmu.edu.ua/article/view/208354/211682 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850242390782115840 |
|---|---|
| author | V. I. Tsymbaliuk I. G. Vasylieva M. R. Kostiuk N. G. Chopyk O. S. Galanta O. I. Tsiubko N. P. Oleksenko A. B. Dmytrenko T. A. Makarova N. D. Snitzar |
| author_facet | V. I. Tsymbaliuk I. G. Vasylieva M. R. Kostiuk N. G. Chopyk O. S. Galanta O. I. Tsiubko N. P. Oleksenko A. B. Dmytrenko T. A. Makarova N. D. Snitzar |
| author_sort | V. I. Tsymbaliuk |
| collection | DOAJ |
| description | The genetic component is a leading factor of stroke manifestation. Contribution of single nucleotide polymorphisms or combined genotypes to disease development has population differences. Special attention should be paid to genetic features in different types of strokes among the Ukrainian population.
The aim of the study was to estimate the association of polymorphisms between FGB, MTHFR, MTR, MTRR genes or gene-gene interaction and a risk of atherotrombotic ischemic stroke and intracranial hemorrhagic stroke among Ukrainians.
Materials and methods. 102 patients with atherotrombotic ischemic stroke, 56 patients with intracranial hemorrhagic stroke and 102 healthy control subjects were included in the study. The control group comprised peoples without cardiovascular diseases, family history of stroke and with normal lipid profile values. The mean age was 53.4 ± 9.1 years for stroke patients and 54.5 ± 8.2 years for the control group. Single nucleotide polymorphisms С677T (rs1801133) MTHFR gene, A66G (rs1801394) MTRR gene, A2756G (rs 1805087) MTR gene, C-148T (rs 1800787) FGB gene were detected by polymerase chain reaction (PCR) followed by restriction of amplificated fragments and polymorphism A1298C (rs1801131) MTHFR gene was determined by allele specific PCR method.
Results. The association between 1298СС genotype MTHFR gene (OR = 3.457, 95 % CI:1.053–11.357) and -148СТ genotype FGB gene (OR = 2.276, 95 % CI: 1.248–4.152) and a risk of atherotrombotic ischemic stroke was revealed. A risk of intracranial hemorrhagic stroke was associated with 66AG (OR = 2.643, 95 % CI: 1.059–6.593) and 66GG (OR = 4.826, 95 % CI: 1.858–12.535) genotypes of MTRR gene. Synergistic effect was shown for polymorphic loci of FGB/С-148Т and MTRR/A66G in atherotrombotic ischemic stroke development as well as independent effect of MTRR/A66G for intracranial hemorrhagic stroke.
Conclusions. There are some differences in the genetic components of different types of acute stroke in Ukrainians that should be taken into account when initiating therapy or preventative measures. |
| format | Article |
| id | doaj-art-b9b7b0972f224a57be4a53925dfcfc9f |
| institution | OA Journals |
| issn | 2306-4145 2310-1210 |
| language | English |
| publishDate | 2020-08-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Zaporožskij Medicinskij Žurnal |
| spelling | doaj-art-b9b7b0972f224a57be4a53925dfcfc9f2025-08-20T02:00:18ZengZaporizhzhia State Medical and Pharmaceutical UniversityZaporožskij Medicinskij Žurnal2306-41452310-12102020-08-0122445946710.14739/2310-1210.2020.4.208354Analysis of association between FGB, MTHFR, MTR and MTRR genes polymorphisms and ischemic and hemorrhagic strokeV. I. Tsymbaliuk0https://orcid.org/0000-0001-7544-6603I. G. Vasylieva1https://orcid.org/0000-0003-4321-5354M. R. Kostiuk2https://orcid.org/0000-0002-6507-5914N. G. Chopyk3https://orcid.org/0000-0003-1024-1556 O. S. Galanta4https://orcid.org/0000-0002-9902-9916O. I. Tsiubko5https://orcid.org/0000-0003-0138-4643N. P. Oleksenko 6https://orcid.org/0000-0002-3267-4961A. B. Dmytrenko7https://orcid.org/0000-0002-0141-3697T. A. Makarova8https://orcid.org/0000-0001-5203-450XN. D. Snitzar9https://orcid.org/0000-0002-3485-2426 State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv. State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv. State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv. State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv. State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv. State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv. State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv. State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv. State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv. State Institution “Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine”, Kyiv.The genetic component is a leading factor of stroke manifestation. Contribution of single nucleotide polymorphisms or combined genotypes to disease development has population differences. Special attention should be paid to genetic features in different types of strokes among the Ukrainian population. The aim of the study was to estimate the association of polymorphisms between FGB, MTHFR, MTR, MTRR genes or gene-gene interaction and a risk of atherotrombotic ischemic stroke and intracranial hemorrhagic stroke among Ukrainians. Materials and methods. 102 patients with atherotrombotic ischemic stroke, 56 patients with intracranial hemorrhagic stroke and 102 healthy control subjects were included in the study. The control group comprised peoples without cardiovascular diseases, family history of stroke and with normal lipid profile values. The mean age was 53.4 ± 9.1 years for stroke patients and 54.5 ± 8.2 years for the control group. Single nucleotide polymorphisms С677T (rs1801133) MTHFR gene, A66G (rs1801394) MTRR gene, A2756G (rs 1805087) MTR gene, C-148T (rs 1800787) FGB gene were detected by polymerase chain reaction (PCR) followed by restriction of amplificated fragments and polymorphism A1298C (rs1801131) MTHFR gene was determined by allele specific PCR method. Results. The association between 1298СС genotype MTHFR gene (OR = 3.457, 95 % CI:1.053–11.357) and -148СТ genotype FGB gene (OR = 2.276, 95 % CI: 1.248–4.152) and a risk of atherotrombotic ischemic stroke was revealed. A risk of intracranial hemorrhagic stroke was associated with 66AG (OR = 2.643, 95 % CI: 1.059–6.593) and 66GG (OR = 4.826, 95 % CI: 1.858–12.535) genotypes of MTRR gene. Synergistic effect was shown for polymorphic loci of FGB/С-148Т and MTRR/A66G in atherotrombotic ischemic stroke development as well as independent effect of MTRR/A66G for intracranial hemorrhagic stroke. Conclusions. There are some differences in the genetic components of different types of acute stroke in Ukrainians that should be taken into account when initiating therapy or preventative measures.http://zmj.zsmu.edu.ua/article/view/208354/211682genetic polymorphismstrokerisk factors |
| spellingShingle | V. I. Tsymbaliuk I. G. Vasylieva M. R. Kostiuk N. G. Chopyk O. S. Galanta O. I. Tsiubko N. P. Oleksenko A. B. Dmytrenko T. A. Makarova N. D. Snitzar Analysis of association between FGB, MTHFR, MTR and MTRR genes polymorphisms and ischemic and hemorrhagic stroke Zaporožskij Medicinskij Žurnal genetic polymorphism stroke risk factors |
| title | Analysis of association between FGB, MTHFR, MTR and MTRR genes polymorphisms and ischemic and hemorrhagic stroke |
| title_full | Analysis of association between FGB, MTHFR, MTR and MTRR genes polymorphisms and ischemic and hemorrhagic stroke |
| title_fullStr | Analysis of association between FGB, MTHFR, MTR and MTRR genes polymorphisms and ischemic and hemorrhagic stroke |
| title_full_unstemmed | Analysis of association between FGB, MTHFR, MTR and MTRR genes polymorphisms and ischemic and hemorrhagic stroke |
| title_short | Analysis of association between FGB, MTHFR, MTR and MTRR genes polymorphisms and ischemic and hemorrhagic stroke |
| title_sort | analysis of association between fgb mthfr mtr and mtrr genes polymorphisms and ischemic and hemorrhagic stroke |
| topic | genetic polymorphism stroke risk factors |
| url | http://zmj.zsmu.edu.ua/article/view/208354/211682 |
| work_keys_str_mv | AT vitsymbaliuk analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke AT igvasylieva analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke AT mrkostiuk analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke AT ngchopyk analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke AT osgalanta analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke AT oitsiubko analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke AT npoleksenko analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke AT abdmytrenko analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke AT tamakarova analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke AT ndsnitzar analysisofassociationbetweenfgbmthfrmtrandmtrrgenespolymorphismsandischemicandhemorrhagicstroke |