Impact of different 3D regions of interest on quantifying dynamic lumbar vertebral microstructure in ovariectomized rats—a micro-CT study
IntroductionThe selection of regions of interest (ROIs) is crucial for accurate microcomputed tomography (micro-CT) analysis. Distinct ROI selection methods exist for lumbar vertebras in osteoporotic animal model research. However, whether different ROIs directly affect the results of quantitative m...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2024.1503761/full |
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| author | Huihui Xu Huihui Xu Hong Liu Meijie Liu Yan Li Jinghua Pan Shaojun Wang Guowei Wang Xin Liu Ying Liu Xiaoqin Hou Hongyan Zhao |
| author_facet | Huihui Xu Huihui Xu Hong Liu Meijie Liu Yan Li Jinghua Pan Shaojun Wang Guowei Wang Xin Liu Ying Liu Xiaoqin Hou Hongyan Zhao |
| author_sort | Huihui Xu |
| collection | DOAJ |
| description | IntroductionThe selection of regions of interest (ROIs) is crucial for accurate microcomputed tomography (micro-CT) analysis. Distinct ROI selection methods exist for lumbar vertebras in osteoporotic animal model research. However, whether different ROIs directly affect the results of quantitative micro-CT-based microarchitectural data is still unknown. This study aimed to compare the diagnostic accuracy of two commonly used ROIs of lumbar vertebras in ovariectomized (OVX) rats at different time points.MethodsRats were randomly divided into the baseline group, the sham/OVX-operated groups, with 12- or 24-weeks sham (Sham 12w or Sham 24w)/12- or 24-weeks (OVX 12w or OVX 24w)-operated group (n = 6 in every group). The fifth lumbar vertebras were collected and scanned using micro-CT. Quantitative analyses of bone microarchitecture parameters were conducted separately for the central ROI (ROI 1) and overall ROI (ROI 2).ResultsThe results indicated that the Tb.N of baseline group rats for ROI 1 was significantly lower than that for ROI 2. The Tb.Th of rats of the Sham 12w and Sham 24w groups was significantly increased compared to that of the baseline group rats using the ROI 2 analysis. The bone mineral density (BMD) and bone volume fraction (BV/TV) were significantly lower by the ROI 1 than by the ROI 2 in all groups. The BMD and BV/TV also showed a significant reduction at 24 weeks postoperatively compared with those at 12 weeks postoperatively. Bland–Altman analysis showed good consistency between the two different ROI selection methods.ConclusionThis study found that capturing peripheral trabeculas (overall ROI) does not explain the increased Tb.Th in healthy mice and decreased Tb.N in OVX mice; both findings indicate that this is evident in both ROI. Moreover, this study suggested the potential value of the central ROI (effective and quicker) for evaluating osteoporosis of the lumbar vertebras in OVX rats and provides a basis for analyzing the morphological changes of lumbar trabecular. |
| format | Article |
| id | doaj-art-b9b50e5f5b4a417bb19b6c2fc51e8b7f |
| institution | DOAJ |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj-art-b9b50e5f5b4a417bb19b6c2fc51e8b7f2025-08-20T02:48:16ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-02-011110.3389/fmed.2024.15037611503761Impact of different 3D regions of interest on quantifying dynamic lumbar vertebral microstructure in ovariectomized rats—a micro-CT studyHuihui Xu0Huihui Xu1Hong Liu2Meijie Liu3Yan Li4Jinghua Pan5Shaojun Wang6Guowei Wang7Xin Liu8Ying Liu9Xiaoqin Hou10Hongyan Zhao11Department of Joints and Soft Tissue Injury, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaBeijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Science, Beijing, ChinaBeijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Science, Beijing, ChinaBeijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Science, Beijing, ChinaBeijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Science, Beijing, ChinaBeijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Science, Beijing, ChinaBeijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Science, Beijing, ChinaInstitute of Basic Theory of Chinese Medicine, China Academy of Chinese Medical Science, Beijing, ChinaBeijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Science, Beijing, ChinaFangta Hospital of Traditional Chinese Medicine, Shanghai, ChinaDepartment of Clinic No. 1 Office, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaBeijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Science, Beijing, ChinaIntroductionThe selection of regions of interest (ROIs) is crucial for accurate microcomputed tomography (micro-CT) analysis. Distinct ROI selection methods exist for lumbar vertebras in osteoporotic animal model research. However, whether different ROIs directly affect the results of quantitative micro-CT-based microarchitectural data is still unknown. This study aimed to compare the diagnostic accuracy of two commonly used ROIs of lumbar vertebras in ovariectomized (OVX) rats at different time points.MethodsRats were randomly divided into the baseline group, the sham/OVX-operated groups, with 12- or 24-weeks sham (Sham 12w or Sham 24w)/12- or 24-weeks (OVX 12w or OVX 24w)-operated group (n = 6 in every group). The fifth lumbar vertebras were collected and scanned using micro-CT. Quantitative analyses of bone microarchitecture parameters were conducted separately for the central ROI (ROI 1) and overall ROI (ROI 2).ResultsThe results indicated that the Tb.N of baseline group rats for ROI 1 was significantly lower than that for ROI 2. The Tb.Th of rats of the Sham 12w and Sham 24w groups was significantly increased compared to that of the baseline group rats using the ROI 2 analysis. The bone mineral density (BMD) and bone volume fraction (BV/TV) were significantly lower by the ROI 1 than by the ROI 2 in all groups. The BMD and BV/TV also showed a significant reduction at 24 weeks postoperatively compared with those at 12 weeks postoperatively. Bland–Altman analysis showed good consistency between the two different ROI selection methods.ConclusionThis study found that capturing peripheral trabeculas (overall ROI) does not explain the increased Tb.Th in healthy mice and decreased Tb.N in OVX mice; both findings indicate that this is evident in both ROI. Moreover, this study suggested the potential value of the central ROI (effective and quicker) for evaluating osteoporosis of the lumbar vertebras in OVX rats and provides a basis for analyzing the morphological changes of lumbar trabecular.https://www.frontiersin.org/articles/10.3389/fmed.2024.1503761/fullmicro-CTregions of interestovariectomylumbar vertebraeosteoporosis |
| spellingShingle | Huihui Xu Huihui Xu Hong Liu Meijie Liu Yan Li Jinghua Pan Shaojun Wang Guowei Wang Xin Liu Ying Liu Xiaoqin Hou Hongyan Zhao Impact of different 3D regions of interest on quantifying dynamic lumbar vertebral microstructure in ovariectomized rats—a micro-CT study Frontiers in Medicine micro-CT regions of interest ovariectomy lumbar vertebrae osteoporosis |
| title | Impact of different 3D regions of interest on quantifying dynamic lumbar vertebral microstructure in ovariectomized rats—a micro-CT study |
| title_full | Impact of different 3D regions of interest on quantifying dynamic lumbar vertebral microstructure in ovariectomized rats—a micro-CT study |
| title_fullStr | Impact of different 3D regions of interest on quantifying dynamic lumbar vertebral microstructure in ovariectomized rats—a micro-CT study |
| title_full_unstemmed | Impact of different 3D regions of interest on quantifying dynamic lumbar vertebral microstructure in ovariectomized rats—a micro-CT study |
| title_short | Impact of different 3D regions of interest on quantifying dynamic lumbar vertebral microstructure in ovariectomized rats—a micro-CT study |
| title_sort | impact of different 3d regions of interest on quantifying dynamic lumbar vertebral microstructure in ovariectomized rats a micro ct study |
| topic | micro-CT regions of interest ovariectomy lumbar vertebrae osteoporosis |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2024.1503761/full |
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