Antioxidant Activity and Cytotoxicity of Baru Nut Oil (<i>Dipteryx alata</i> Vogel) Nanoemulsion in Human Cells
Baru nut oil (<i>Dipteryx alata</i> Vogel) is a lipidic extract from a species endemic to the Cerrado biome, renowned for its antioxidant potential. This study aimed to develop a nanoemulsion containing baru nut oil (BNON) using lecithin and polysorbate 80, and to evaluate its antioxidan...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Applied Nano |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-3501/6/1/3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850204883920093184 |
|---|---|
| author | José Queiroz Arthur Figueredo Bruno Silva Sá Daniel Carneiro Moreira João Bueno Nunes Peter Eaton José Roberto Souza de Almeida Leite Andreanne Gomes Vasconcelos |
| author_facet | José Queiroz Arthur Figueredo Bruno Silva Sá Daniel Carneiro Moreira João Bueno Nunes Peter Eaton José Roberto Souza de Almeida Leite Andreanne Gomes Vasconcelos |
| author_sort | José Queiroz |
| collection | DOAJ |
| description | Baru nut oil (<i>Dipteryx alata</i> Vogel) is a lipidic extract from a species endemic to the Cerrado biome, renowned for its antioxidant potential. This study aimed to develop a nanoemulsion containing baru nut oil (BNON) using lecithin and polysorbate 80, and to evaluate its antioxidant activity and cytotoxicity. The physicochemical properties of BNON were characterized, and its cytotoxicity was assessed in human erythrocytes and keratinocytes. Antioxidant activity was evaluated using the DPPH method and inhibition of AAPH-induced hemolysis. BNON exhibited a droplet size of 530.1 ± 20.48 nm, a polydispersity index of 0.496 ± 0.057, and a zeta potential of −35.7 ± 2.19 mV. Free baru nut oil showed no cytotoxicity to keratinocytes or erythrocytes within the concentration ranges tested (1.0–0.031 mg/mL and 0.8–0.006 mg/mL, respectively). In contrast, BNON displayed cytotoxic effects on keratinocytes and erythrocytes only at the highest tested concentration. Atomic force microscopy analysis of erythrocytes from the hemolysis assay revealed normal morphology for cells treated with free oil at 0.8 mg/mL, whereas cells treated with BNON at the same concentration exhibited a slightly widened concave center. Free oil at 0.8 mg/mL significantly protected erythrocytes from AAPH-induced hemolysis, while BNON did not. However, BNON (5 mg/mL) demonstrated free radical scavenging activity, quantified at 0.0074 mg Trolox equivalents/mg via the DPPH assay. These findings suggest that baru nut oil has potential as an antioxidant product, although further optimization of the nanoformulation is required. |
| format | Article |
| id | doaj-art-b9b4c35d6b9b44ac93e83bf97104a7d4 |
| institution | OA Journals |
| issn | 2673-3501 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Nano |
| spelling | doaj-art-b9b4c35d6b9b44ac93e83bf97104a7d42025-08-20T02:11:12ZengMDPI AGApplied Nano2673-35012025-02-0161310.3390/applnano6010003Antioxidant Activity and Cytotoxicity of Baru Nut Oil (<i>Dipteryx alata</i> Vogel) Nanoemulsion in Human CellsJosé Queiroz0Arthur Figueredo1Bruno Silva Sá2Daniel Carneiro Moreira3João Bueno Nunes4Peter Eaton5José Roberto Souza de Almeida Leite6Andreanne Gomes Vasconcelos7Research Center in Morphology and Applied Immunology (NuPMIA), Area of Morphology, Faculty of Medicine (FM), University of Brasilia (UnB), Brasilia 70910-900, BrazilUnified Education Center of the Federal District (UDF), Brasilia 70390045, BrazilResearch Center in Morphology and Applied Immunology (NuPMIA), Area of Morphology, Faculty of Medicine (FM), University of Brasilia (UnB), Brasilia 70910-900, BrazilResearch Center in Morphology and Applied Immunology (NuPMIA), Area of Morphology, Faculty of Medicine (FM), University of Brasilia (UnB), Brasilia 70910-900, BrazilLaboratory for the Synthesis and Analysis of Biomolecules, Institute of Chemistry, University of Brasilia (UnB), Brasília 70910900, BrazilThe Bridge and School of Natural Sciences, University of Lincoln, Lincoln LN6 7EL, UKResearch Center in Morphology and Applied Immunology (NuPMIA), Area of Morphology, Faculty of Medicine (FM), University of Brasilia (UnB), Brasilia 70910-900, BrazilResearch Center in Morphology and Applied Immunology (NuPMIA), Area of Morphology, Faculty of Medicine (FM), University of Brasilia (UnB), Brasilia 70910-900, BrazilBaru nut oil (<i>Dipteryx alata</i> Vogel) is a lipidic extract from a species endemic to the Cerrado biome, renowned for its antioxidant potential. This study aimed to develop a nanoemulsion containing baru nut oil (BNON) using lecithin and polysorbate 80, and to evaluate its antioxidant activity and cytotoxicity. The physicochemical properties of BNON were characterized, and its cytotoxicity was assessed in human erythrocytes and keratinocytes. Antioxidant activity was evaluated using the DPPH method and inhibition of AAPH-induced hemolysis. BNON exhibited a droplet size of 530.1 ± 20.48 nm, a polydispersity index of 0.496 ± 0.057, and a zeta potential of −35.7 ± 2.19 mV. Free baru nut oil showed no cytotoxicity to keratinocytes or erythrocytes within the concentration ranges tested (1.0–0.031 mg/mL and 0.8–0.006 mg/mL, respectively). In contrast, BNON displayed cytotoxic effects on keratinocytes and erythrocytes only at the highest tested concentration. Atomic force microscopy analysis of erythrocytes from the hemolysis assay revealed normal morphology for cells treated with free oil at 0.8 mg/mL, whereas cells treated with BNON at the same concentration exhibited a slightly widened concave center. Free oil at 0.8 mg/mL significantly protected erythrocytes from AAPH-induced hemolysis, while BNON did not. However, BNON (5 mg/mL) demonstrated free radical scavenging activity, quantified at 0.0074 mg Trolox equivalents/mg via the DPPH assay. These findings suggest that baru nut oil has potential as an antioxidant product, although further optimization of the nanoformulation is required.https://www.mdpi.com/2673-3501/6/1/3biosafetyfree radical scavengingnanotechnologynatural productsoxidative stress |
| spellingShingle | José Queiroz Arthur Figueredo Bruno Silva Sá Daniel Carneiro Moreira João Bueno Nunes Peter Eaton José Roberto Souza de Almeida Leite Andreanne Gomes Vasconcelos Antioxidant Activity and Cytotoxicity of Baru Nut Oil (<i>Dipteryx alata</i> Vogel) Nanoemulsion in Human Cells Applied Nano biosafety free radical scavenging nanotechnology natural products oxidative stress |
| title | Antioxidant Activity and Cytotoxicity of Baru Nut Oil (<i>Dipteryx alata</i> Vogel) Nanoemulsion in Human Cells |
| title_full | Antioxidant Activity and Cytotoxicity of Baru Nut Oil (<i>Dipteryx alata</i> Vogel) Nanoemulsion in Human Cells |
| title_fullStr | Antioxidant Activity and Cytotoxicity of Baru Nut Oil (<i>Dipteryx alata</i> Vogel) Nanoemulsion in Human Cells |
| title_full_unstemmed | Antioxidant Activity and Cytotoxicity of Baru Nut Oil (<i>Dipteryx alata</i> Vogel) Nanoemulsion in Human Cells |
| title_short | Antioxidant Activity and Cytotoxicity of Baru Nut Oil (<i>Dipteryx alata</i> Vogel) Nanoemulsion in Human Cells |
| title_sort | antioxidant activity and cytotoxicity of baru nut oil i dipteryx alata i vogel nanoemulsion in human cells |
| topic | biosafety free radical scavenging nanotechnology natural products oxidative stress |
| url | https://www.mdpi.com/2673-3501/6/1/3 |
| work_keys_str_mv | AT josequeiroz antioxidantactivityandcytotoxicityofbarunutoilidipteryxalataivogelnanoemulsioninhumancells AT arthurfigueredo antioxidantactivityandcytotoxicityofbarunutoilidipteryxalataivogelnanoemulsioninhumancells AT brunosilvasa antioxidantactivityandcytotoxicityofbarunutoilidipteryxalataivogelnanoemulsioninhumancells AT danielcarneiromoreira antioxidantactivityandcytotoxicityofbarunutoilidipteryxalataivogelnanoemulsioninhumancells AT joaobuenonunes antioxidantactivityandcytotoxicityofbarunutoilidipteryxalataivogelnanoemulsioninhumancells AT petereaton antioxidantactivityandcytotoxicityofbarunutoilidipteryxalataivogelnanoemulsioninhumancells AT joserobertosouzadealmeidaleite antioxidantactivityandcytotoxicityofbarunutoilidipteryxalataivogelnanoemulsioninhumancells AT andreannegomesvasconcelos antioxidantactivityandcytotoxicityofbarunutoilidipteryxalataivogelnanoemulsioninhumancells |