Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria
Aim. To determine the antimicrobial potential of guava (Psidium guajava) leaf extracts against two gram-negative bacteria (Escherichia coli and Salmonella enteritidis) and two gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) which are some of foodborne and spoilage bacteria. The gu...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2013/746165 |
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| author | Bipul Biswas Kimberly Rogers Fredrick McLaughlin Dwayne Daniels Anand Yadav |
| author_facet | Bipul Biswas Kimberly Rogers Fredrick McLaughlin Dwayne Daniels Anand Yadav |
| author_sort | Bipul Biswas |
| collection | DOAJ |
| description | Aim. To determine the antimicrobial potential of guava (Psidium guajava) leaf extracts against two gram-negative bacteria (Escherichia coli and Salmonella enteritidis) and two gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) which are some of foodborne and spoilage bacteria. The guava leaves were extracted in four different solvents of increasing polarities (hexane, methanol, ethanol, and water). The efficacy of these extracts was tested against those bacteria through a well-diffusion method employing 50 μL leaf-extract solution per well. According to the findings of the antibacterial assay, the methanol and ethanol extracts of the guava leaves showed inhibitory activity against gram-positive bacteria, whereas the gram-negative bacteria were resistant to all the solvent extracts. The methanol extract had an antibacterial activity with mean zones of inhibition of 8.27 and 12.3 mm, and the ethanol extract had a mean zone of inhibition of 6.11 and 11.0 mm against B. cereus and S. aureus, respectively. On the basis of the present finding, guava leaf-extract might be a good candidate in the search for a natural antimicrobial agent. This study provides scientific understanding to further determine the antimicrobial values and investigate other pharmacological properties. |
| format | Article |
| id | doaj-art-b9a743b351924dfa992ea36d5cbda472 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-b9a743b351924dfa992ea36d5cbda4722025-02-03T05:47:55ZengWileyInternational Journal of Microbiology1687-918X1687-91982013-01-01201310.1155/2013/746165746165Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive BacteriaBipul Biswas0Kimberly Rogers1Fredrick McLaughlin2Dwayne Daniels3Anand Yadav4MS Biotechnology Program, College of Agriculture, Family Sciences and Technology, Fort Valley State University, Fort Valley, GA 31030, USAMS Biotechnology Program, College of Agriculture, Family Sciences and Technology, Fort Valley State University, Fort Valley, GA 31030, USADepartment of Biology, Fort Valley State University, Fort Valley, GA 31030, USADepartment of Chemistry, Fort Valley State University, Fort Valley, GA 31030, USAMS Biotechnology Program, College of Agriculture, Family Sciences and Technology, Fort Valley State University, Fort Valley, GA 31030, USAAim. To determine the antimicrobial potential of guava (Psidium guajava) leaf extracts against two gram-negative bacteria (Escherichia coli and Salmonella enteritidis) and two gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) which are some of foodborne and spoilage bacteria. The guava leaves were extracted in four different solvents of increasing polarities (hexane, methanol, ethanol, and water). The efficacy of these extracts was tested against those bacteria through a well-diffusion method employing 50 μL leaf-extract solution per well. According to the findings of the antibacterial assay, the methanol and ethanol extracts of the guava leaves showed inhibitory activity against gram-positive bacteria, whereas the gram-negative bacteria were resistant to all the solvent extracts. The methanol extract had an antibacterial activity with mean zones of inhibition of 8.27 and 12.3 mm, and the ethanol extract had a mean zone of inhibition of 6.11 and 11.0 mm against B. cereus and S. aureus, respectively. On the basis of the present finding, guava leaf-extract might be a good candidate in the search for a natural antimicrobial agent. This study provides scientific understanding to further determine the antimicrobial values and investigate other pharmacological properties.http://dx.doi.org/10.1155/2013/746165 |
| spellingShingle | Bipul Biswas Kimberly Rogers Fredrick McLaughlin Dwayne Daniels Anand Yadav Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria International Journal of Microbiology |
| title | Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria |
| title_full | Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria |
| title_fullStr | Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria |
| title_full_unstemmed | Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria |
| title_short | Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria |
| title_sort | antimicrobial activities of leaf extracts of guava psidium guajava l on two gram negative and gram positive bacteria |
| url | http://dx.doi.org/10.1155/2013/746165 |
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