Transcriptomic Analysis Provides New Insights into Oocyte Growth and Maturation in Greater Amberjack (<i>Seriola dumerili</i>)
The greater amberjack (<i>Seriola dumerili</i>) is an emerging marine fish that is increasingly favored in aquaculture. Currently, there are few studies on the development and regulation of greater amberjack ovaries. In this study, the ovary transcriptome profiles of greater amberjack at...
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2025-01-01
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| author | Jiahui Yang Xiaoying Ru Yang Huang Jinhui Wu Tonglin Yang Peipei Chen Jin Li Kunfeng Zhu Chunhua Zhu |
| author_facet | Jiahui Yang Xiaoying Ru Yang Huang Jinhui Wu Tonglin Yang Peipei Chen Jin Li Kunfeng Zhu Chunhua Zhu |
| author_sort | Jiahui Yang |
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| description | The greater amberjack (<i>Seriola dumerili</i>) is an emerging marine fish that is increasingly favored in aquaculture. Currently, there are few studies on the development and regulation of greater amberjack ovaries. In this study, the ovary transcriptome profiles of greater amberjack at three different stages (stage II, III, and IV) were performed, and identified the genes and pathways that may play significant roles in the processes of follicle growth and maturation. A total of 6597, and 1061 differentially expressed genes (DEGs) were detected in FII vs. FIII, FIII vs. FIV, and FII vs. FIV stages, respectively. GO and KEGG enrichment analyses revealed that these DEG<sub>S</sub> are primarily involved in steroid hormone biosynthesis (e.g., <i>cyp11a1</i>, <i>cyp17a1</i>, <i>cyp19a1a</i>, <i>hsd3b1</i>, <i>esr1</i>), lipid metabolism (e.g., <i>plpp3</i>, <i>lpl</i>, <i>pld1,</i> and <i>fabp10a</i>), and meiotic arrest and resumption (e.g., <i>pgr</i>, <i>arb</i>, <i>ccnd2</i>, <i>adcy2</i>, <i>adcy9</i>, <i>myl9</i>, <i>calm1</i>). Additionally, several signaling pathways involved in ovarian development have been identified, including the PI3K-Akt, Wnt, TGF-beta, GnRH, and immune-related signaling pathways. qPCR results of nine representative genes related to steroid hormone synthesis and cell growth verified the reliability of the generated RNA-seq data. This research contributes to our comprehension of the molecular processes underlying ovarian growth and maturation in marine fishes and provides a theoretical basis for the investigation of functional genes associated with oogenesis in greater amberjack. |
| format | Article |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-b99c85cd95cf43579dc887b42053b07d2025-08-20T02:48:09ZengMDPI AGAnimals2076-26152025-01-0115333310.3390/ani15030333Transcriptomic Analysis Provides New Insights into Oocyte Growth and Maturation in Greater Amberjack (<i>Seriola dumerili</i>)Jiahui Yang0Xiaoying Ru1Yang Huang2Jinhui Wu3Tonglin Yang4Peipei Chen5Jin Li6Kunfeng Zhu7Chunhua Zhu8Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaDevelopment and Research Center for Biological Marine Resources, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524025, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaAgro-Tech Extension Center of Guangdong Province, Guangzhou 510500, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaGuangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Fisheries College of Guangdong Ocean University, Zhanjiang 524088, ChinaThe greater amberjack (<i>Seriola dumerili</i>) is an emerging marine fish that is increasingly favored in aquaculture. Currently, there are few studies on the development and regulation of greater amberjack ovaries. In this study, the ovary transcriptome profiles of greater amberjack at three different stages (stage II, III, and IV) were performed, and identified the genes and pathways that may play significant roles in the processes of follicle growth and maturation. A total of 6597, and 1061 differentially expressed genes (DEGs) were detected in FII vs. FIII, FIII vs. FIV, and FII vs. FIV stages, respectively. GO and KEGG enrichment analyses revealed that these DEG<sub>S</sub> are primarily involved in steroid hormone biosynthesis (e.g., <i>cyp11a1</i>, <i>cyp17a1</i>, <i>cyp19a1a</i>, <i>hsd3b1</i>, <i>esr1</i>), lipid metabolism (e.g., <i>plpp3</i>, <i>lpl</i>, <i>pld1,</i> and <i>fabp10a</i>), and meiotic arrest and resumption (e.g., <i>pgr</i>, <i>arb</i>, <i>ccnd2</i>, <i>adcy2</i>, <i>adcy9</i>, <i>myl9</i>, <i>calm1</i>). Additionally, several signaling pathways involved in ovarian development have been identified, including the PI3K-Akt, Wnt, TGF-beta, GnRH, and immune-related signaling pathways. qPCR results of nine representative genes related to steroid hormone synthesis and cell growth verified the reliability of the generated RNA-seq data. This research contributes to our comprehension of the molecular processes underlying ovarian growth and maturation in marine fishes and provides a theoretical basis for the investigation of functional genes associated with oogenesis in greater amberjack.https://www.mdpi.com/2076-2615/15/3/333<i>Seriola dumerili</i>different developmental stagesovarytranscriptomic analysis |
| spellingShingle | Jiahui Yang Xiaoying Ru Yang Huang Jinhui Wu Tonglin Yang Peipei Chen Jin Li Kunfeng Zhu Chunhua Zhu Transcriptomic Analysis Provides New Insights into Oocyte Growth and Maturation in Greater Amberjack (<i>Seriola dumerili</i>) Animals <i>Seriola dumerili</i> different developmental stages ovary transcriptomic analysis |
| title | Transcriptomic Analysis Provides New Insights into Oocyte Growth and Maturation in Greater Amberjack (<i>Seriola dumerili</i>) |
| title_full | Transcriptomic Analysis Provides New Insights into Oocyte Growth and Maturation in Greater Amberjack (<i>Seriola dumerili</i>) |
| title_fullStr | Transcriptomic Analysis Provides New Insights into Oocyte Growth and Maturation in Greater Amberjack (<i>Seriola dumerili</i>) |
| title_full_unstemmed | Transcriptomic Analysis Provides New Insights into Oocyte Growth and Maturation in Greater Amberjack (<i>Seriola dumerili</i>) |
| title_short | Transcriptomic Analysis Provides New Insights into Oocyte Growth and Maturation in Greater Amberjack (<i>Seriola dumerili</i>) |
| title_sort | transcriptomic analysis provides new insights into oocyte growth and maturation in greater amberjack i seriola dumerili i |
| topic | <i>Seriola dumerili</i> different developmental stages ovary transcriptomic analysis |
| url | https://www.mdpi.com/2076-2615/15/3/333 |
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