Changes in Amplitude-integrated Electroencephalograms in Piglets During Selective Mild Head Cooling After Hypoxia-ischemia

Amplitude-integrated electroencephalogram (aEEG) is a simplified, alternative means of monitoring cerebral function and may be more useful clinically in some situations than conventional EEG. The aim of this study is to evaluate newborn piglets as an animal model to examine the effect of selective m...

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Main Authors: Ji-Mei Wang, Guo-Fu Zhang, Wen-Hao Zhou, Ze-Dong Jiang, Xiao-Mei Shao
Format: Article
Language:English
Published: Elsevier 2014-08-01
Series:Pediatrics and Neonatology
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Online Access:http://www.sciencedirect.com/science/article/pii/S1875957213002271
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author Ji-Mei Wang
Guo-Fu Zhang
Wen-Hao Zhou
Ze-Dong Jiang
Xiao-Mei Shao
author_facet Ji-Mei Wang
Guo-Fu Zhang
Wen-Hao Zhou
Ze-Dong Jiang
Xiao-Mei Shao
author_sort Ji-Mei Wang
collection DOAJ
description Amplitude-integrated electroencephalogram (aEEG) is a simplified, alternative means of monitoring cerebral function and may be more useful clinically in some situations than conventional EEG. The aim of this study is to evaluate newborn piglets as an animal model to examine the effect of selective mild head cooling (HC) on aEEG after hypoxia-ischemia (HI). Methods: Thirty-four piglets were randomly allocated to the following treatment groups: normothermic control group (NC, n = 7), selective HC control group (HC, n = 9), normothermic HI group (NHI, n = 9), and selective HC HI group (SHC-HI, n = 9). HI was induced by temporary occlusion of both carotid arteries and simultaneous reduction of the concentration of inspired oxygen to 6% for 30 minutes. Mild hypothermia (35°C) was induced after HI using a HC cap and was maintained for 24 hours. Changes in aEEG were monitored for 6 days after these treatments and the incidence of abnormalities analyzed. Physiological parameters were also measured during this period. Results: In the two HI groups, animals exhibited severely abnormal aEEGs [continuous low voltage (CLV), burst-suppression, or flat tracing (FT)] 20 minutes after the beginning of HI. At 2 hours, the aEEG returned to normal in most of these animals. From 12 hours to 6 days, all animals in the NHI group exhibited severely abnormal aEEGs. Fewer animals in the SHC-HI group exhibited severe abnormal aEEGs during this time period, and four out of nine (44.4%) animals had continuous normal voltage (CNV) at 6 days. Conclusions: Selective mild HC decreases the incidence of severe abnormal aEEGs at late times after HI in newborn piglets.
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spelling doaj-art-b9962f28246c4bc087449b54fbffeaa42025-08-20T03:04:07ZengElsevierPediatrics and Neonatology1875-95722014-08-0155428229010.1016/j.pedneo.2013.09.012Changes in Amplitude-integrated Electroencephalograms in Piglets During Selective Mild Head Cooling After Hypoxia-ischemiaJi-Mei Wang0Guo-Fu Zhang1Wen-Hao Zhou2Ze-Dong Jiang3Xiao-Mei Shao4Department of Neonatology, Gynecology, and Obstetrics, Hospital of Fudan University, Shanghai 200011, ChinaDepartment of Radiology, Gynecology, and Obstetrics, Hospital of Fudan University, Shanghai 200011, ChinaDepartment of Neonatology, Children's Hospital of Fudan University, Shanghai 201102, ChinaDepartment of Neonatology, Children's Hospital of Fudan University, Shanghai 201102, ChinaDepartment of Neonatology, Children's Hospital of Fudan University, Shanghai 201102, ChinaAmplitude-integrated electroencephalogram (aEEG) is a simplified, alternative means of monitoring cerebral function and may be more useful clinically in some situations than conventional EEG. The aim of this study is to evaluate newborn piglets as an animal model to examine the effect of selective mild head cooling (HC) on aEEG after hypoxia-ischemia (HI). Methods: Thirty-four piglets were randomly allocated to the following treatment groups: normothermic control group (NC, n = 7), selective HC control group (HC, n = 9), normothermic HI group (NHI, n = 9), and selective HC HI group (SHC-HI, n = 9). HI was induced by temporary occlusion of both carotid arteries and simultaneous reduction of the concentration of inspired oxygen to 6% for 30 minutes. Mild hypothermia (35°C) was induced after HI using a HC cap and was maintained for 24 hours. Changes in aEEG were monitored for 6 days after these treatments and the incidence of abnormalities analyzed. Physiological parameters were also measured during this period. Results: In the two HI groups, animals exhibited severely abnormal aEEGs [continuous low voltage (CLV), burst-suppression, or flat tracing (FT)] 20 minutes after the beginning of HI. At 2 hours, the aEEG returned to normal in most of these animals. From 12 hours to 6 days, all animals in the NHI group exhibited severely abnormal aEEGs. Fewer animals in the SHC-HI group exhibited severe abnormal aEEGs during this time period, and four out of nine (44.4%) animals had continuous normal voltage (CNV) at 6 days. Conclusions: Selective mild HC decreases the incidence of severe abnormal aEEGs at late times after HI in newborn piglets.http://www.sciencedirect.com/science/article/pii/S1875957213002271aEEGbrain damagehypoxia-ischemianeonatologyneuroprotectionpiglets
spellingShingle Ji-Mei Wang
Guo-Fu Zhang
Wen-Hao Zhou
Ze-Dong Jiang
Xiao-Mei Shao
Changes in Amplitude-integrated Electroencephalograms in Piglets During Selective Mild Head Cooling After Hypoxia-ischemia
Pediatrics and Neonatology
aEEG
brain damage
hypoxia-ischemia
neonatology
neuroprotection
piglets
title Changes in Amplitude-integrated Electroencephalograms in Piglets During Selective Mild Head Cooling After Hypoxia-ischemia
title_full Changes in Amplitude-integrated Electroencephalograms in Piglets During Selective Mild Head Cooling After Hypoxia-ischemia
title_fullStr Changes in Amplitude-integrated Electroencephalograms in Piglets During Selective Mild Head Cooling After Hypoxia-ischemia
title_full_unstemmed Changes in Amplitude-integrated Electroencephalograms in Piglets During Selective Mild Head Cooling After Hypoxia-ischemia
title_short Changes in Amplitude-integrated Electroencephalograms in Piglets During Selective Mild Head Cooling After Hypoxia-ischemia
title_sort changes in amplitude integrated electroencephalograms in piglets during selective mild head cooling after hypoxia ischemia
topic aEEG
brain damage
hypoxia-ischemia
neonatology
neuroprotection
piglets
url http://www.sciencedirect.com/science/article/pii/S1875957213002271
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