Alternative DNA Markers to Detect Guam-Specific CRB-G (Clade I) <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae) Indicate That the Beetle Did Not Disperse from Guam to the Solomon Islands or Palau
A partial mitochondrial DNA <i>Cytochrome Oxidase subunit I</i> (mt<i>COI</i>) gene haplotype variant of the coconut rhinoceros beetle (CRB) <i>Oryctes rhinoceros</i>, classed as ‘CRB-G (clade I)’, has been the focus of much research since 2007, with reports of in...
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2024-10-01
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| author | Wee Tek Tay Sean D. G. Marshall Angel David Popa-Baez Glenn F. J. Dulla Andrea L. Blas Juniaty W. Sambiran Meldy Hosang Justine Bennette H. Millado Michael Melzer Rahul V. Rane Tim Hogarty Demi Yi-Chun Cho Jelfina C. Alouw Muhammad Faheem Benjamin D. Hoffmann |
| author_facet | Wee Tek Tay Sean D. G. Marshall Angel David Popa-Baez Glenn F. J. Dulla Andrea L. Blas Juniaty W. Sambiran Meldy Hosang Justine Bennette H. Millado Michael Melzer Rahul V. Rane Tim Hogarty Demi Yi-Chun Cho Jelfina C. Alouw Muhammad Faheem Benjamin D. Hoffmann |
| author_sort | Wee Tek Tay |
| collection | DOAJ |
| description | A partial mitochondrial DNA <i>Cytochrome Oxidase subunit I</i> (mt<i>COI</i>) gene haplotype variant of the coconut rhinoceros beetle (CRB) <i>Oryctes rhinoceros</i>, classed as ‘CRB-G (clade I)’, has been the focus of much research since 2007, with reports of invasions into new Pacific Island locations (e.g., Guam, Hawaii, Solomons Islands). For numerous invasive species, inference of invasion biology via whole genome is superior to assessments via the partial mt<i>COI</i> gene. Here, we explore CRB draft mitochondrial genomes (mitogenomes) from historical and recent collections, with assessment focused on individuals associated within the CRB-G (clade I) classification. We found that all Guam CRB individuals possessed the same mitogenome across all 13 protein-coding genes and differed from individuals collected elsewhere, including ‘non-Guam’ individuals designated as CRB-G (clade I) by partial mt<i>COI</i> assessment. Two alternative <i>ATP6</i> and <i>COIII</i> partial gene primer sets were developed to enable distinction between CRB individuals from Guam that classed within the CRB-G (clade I) haplotype grouping and CRB-G (Clade I) individuals collected elsewhere. Phylogenetic analyses based on concatenated <i>ATP6–COIII</i> genes showed that only Guam CRB-G (clade I) individuals clustered together, and therefore Guam was not the source of the CRB that invaded the other locations in the Pacific assessed in this study. The use of the mt<i>COI</i> and/or mt<i>COIII</i> genes for initial molecular diagnosis of CRB remained crucial, and assessment of more native CRB populations will further advance our ability to identify the provenance of CRB invasions being reported within the Pacific and elsewhere. |
| format | Article |
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| issn | 1424-2818 |
| language | English |
| publishDate | 2024-10-01 |
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| spelling | doaj-art-b99093da758946d18e3e40f1d2e83f0f2025-08-20T02:11:05ZengMDPI AGDiversity1424-28182024-10-01161063410.3390/d16100634Alternative DNA Markers to Detect Guam-Specific CRB-G (Clade I) <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae) Indicate That the Beetle Did Not Disperse from Guam to the Solomon Islands or PalauWee Tek Tay0Sean D. G. Marshall1Angel David Popa-Baez2Glenn F. J. Dulla3Andrea L. Blas4Juniaty W. Sambiran5Meldy Hosang6Justine Bennette H. Millado7Michael Melzer8Rahul V. Rane9Tim Hogarty10Demi Yi-Chun Cho11Jelfina C. Alouw12Muhammad Faheem13Benjamin D. Hoffmann14CSIRO Black Mountain Laboratories, Canberra, ACT 2601, AustraliaAgResearch Limited, Lincoln 7608, New ZealandCSIRO Black Mountain Laboratories, Canberra, ACT 2601, AustraliaCollege of Natural and Applied Sciences, Western Pacific Tropical Research Center, University of Guam, Mangilao 96923, GuamResearch Corporation of the University of Guam, Mangilao 96921, GuamIndonesia Agricultural Instrument Standardization Agency, Kotak Pos 1004, Manado 95001, IndonesiaNational Research & Innovation Agency of Indonesia (BRIN), Jakarta 10340, IndonesiaDepartment of Pest Management, Visayas State University, Leyte 6521, PhilippinesDepartment of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822, USACSIRO, Parkville, VIC 3052, AustraliaCSIRO Marine Labs, Hobart, TAS 7000, AustraliaCSIRO Black Mountain Laboratories, Canberra, ACT 2601, AustraliaNational Research & Innovation Agency of Indonesia (BRIN), Jakarta 10340, IndonesiaCAB International Southeast Asia, Serdang 43300, Selangor, MalaysiaCSIRO, Tropical Ecosystems Research Centre, Winnellie, NT 0822, AustraliaA partial mitochondrial DNA <i>Cytochrome Oxidase subunit I</i> (mt<i>COI</i>) gene haplotype variant of the coconut rhinoceros beetle (CRB) <i>Oryctes rhinoceros</i>, classed as ‘CRB-G (clade I)’, has been the focus of much research since 2007, with reports of invasions into new Pacific Island locations (e.g., Guam, Hawaii, Solomons Islands). For numerous invasive species, inference of invasion biology via whole genome is superior to assessments via the partial mt<i>COI</i> gene. Here, we explore CRB draft mitochondrial genomes (mitogenomes) from historical and recent collections, with assessment focused on individuals associated within the CRB-G (clade I) classification. We found that all Guam CRB individuals possessed the same mitogenome across all 13 protein-coding genes and differed from individuals collected elsewhere, including ‘non-Guam’ individuals designated as CRB-G (clade I) by partial mt<i>COI</i> assessment. Two alternative <i>ATP6</i> and <i>COIII</i> partial gene primer sets were developed to enable distinction between CRB individuals from Guam that classed within the CRB-G (clade I) haplotype grouping and CRB-G (Clade I) individuals collected elsewhere. Phylogenetic analyses based on concatenated <i>ATP6–COIII</i> genes showed that only Guam CRB-G (clade I) individuals clustered together, and therefore Guam was not the source of the CRB that invaded the other locations in the Pacific assessed in this study. The use of the mt<i>COI</i> and/or mt<i>COIII</i> genes for initial molecular diagnosis of CRB remained crucial, and assessment of more native CRB populations will further advance our ability to identify the provenance of CRB invasions being reported within the Pacific and elsewhere.https://www.mdpi.com/1424-2818/16/10/634DNA barcodingcomparative mitogenome analysisAsiatic rhinoceros beetlehitchhiker plant pest |
| spellingShingle | Wee Tek Tay Sean D. G. Marshall Angel David Popa-Baez Glenn F. J. Dulla Andrea L. Blas Juniaty W. Sambiran Meldy Hosang Justine Bennette H. Millado Michael Melzer Rahul V. Rane Tim Hogarty Demi Yi-Chun Cho Jelfina C. Alouw Muhammad Faheem Benjamin D. Hoffmann Alternative DNA Markers to Detect Guam-Specific CRB-G (Clade I) <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae) Indicate That the Beetle Did Not Disperse from Guam to the Solomon Islands or Palau Diversity DNA barcoding comparative mitogenome analysis Asiatic rhinoceros beetle hitchhiker plant pest |
| title | Alternative DNA Markers to Detect Guam-Specific CRB-G (Clade I) <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae) Indicate That the Beetle Did Not Disperse from Guam to the Solomon Islands or Palau |
| title_full | Alternative DNA Markers to Detect Guam-Specific CRB-G (Clade I) <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae) Indicate That the Beetle Did Not Disperse from Guam to the Solomon Islands or Palau |
| title_fullStr | Alternative DNA Markers to Detect Guam-Specific CRB-G (Clade I) <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae) Indicate That the Beetle Did Not Disperse from Guam to the Solomon Islands or Palau |
| title_full_unstemmed | Alternative DNA Markers to Detect Guam-Specific CRB-G (Clade I) <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae) Indicate That the Beetle Did Not Disperse from Guam to the Solomon Islands or Palau |
| title_short | Alternative DNA Markers to Detect Guam-Specific CRB-G (Clade I) <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae) Indicate That the Beetle Did Not Disperse from Guam to the Solomon Islands or Palau |
| title_sort | alternative dna markers to detect guam specific crb g clade i i oryctes rhinoceros i coleoptera scarabaeidae indicate that the beetle did not disperse from guam to the solomon islands or palau |
| topic | DNA barcoding comparative mitogenome analysis Asiatic rhinoceros beetle hitchhiker plant pest |
| url | https://www.mdpi.com/1424-2818/16/10/634 |
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