ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCE
Two hundred samples (cow’s and goat’s milk75 for each,their farm soil 25and 25water samples )from different origin have been cultured on Ashdown selective agar which gave a higher percentage (36.5%)for B. pseudomallei isolation , 64% was observed in farm soil followed by water samples 48% than cow’s...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
College of Veterinary Medicine, University of Basrah, Iraq
2012-12-01
|
| Series: | Basrah Journal of Veterinary Research |
| Subjects: | |
| Online Access: | https://bjvr.uobasrah.edu.iq/article_68925_80809305231e3a4a81f7847000c871a5.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849321388808077312 |
|---|---|
| author | Adnan .M . AL-Rodhan Hanaa K. Ibrahim |
| author_facet | Adnan .M . AL-Rodhan Hanaa K. Ibrahim |
| author_sort | Adnan .M . AL-Rodhan Hanaa K. Ibrahim |
| collection | DOAJ |
| description | Two hundred samples (cow’s and goat’s milk75 for each,their farm soil 25and 25water samples )from different origin have been cultured on Ashdown selective agar which gave a higher percentage (36.5%)for B. pseudomallei isolation , 64% was observed in farm soil followed by water samples 48% than cow’s milk33.33% and finally the goat’s milk 26.66%. According to L-arabinose biotyping , 39.72% of the B.pseudomallei isolates were positive for arabinose (Arb+) and 60.3% of isolates were negative for arabinose (Arb-).The virulence testing revealed that most bacterial isolates yield haemolysin, protease, lipase and lecithenase in a percentage of 97.3% , 82.2%, 89.04% and 84.9% respectively.The Arb- biotypes were more virulent than Arb+ biotypes with significant differences (P< 0.05).All bacterial isolates were characterized by their ability to form capsule(100%),.This study confirmed the presence of casein proteolytic and necrotizing enzymes in the suspension of B.pseudomallei .Proteins concentration in this suspension was 0.00248 mg/ml . |
| format | Article |
| id | doaj-art-b98ff8db5a244a548a02c43f20cc33fd |
| institution | Kabale University |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2012-12-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-b98ff8db5a244a548a02c43f20cc33fd2025-08-20T03:49:45ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562012-12-0111214515810.33762/bvetr.2012.6892568925ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCEAdnan .M . AL-Rodhan Hanaa K. IbrahimTwo hundred samples (cow’s and goat’s milk75 for each,their farm soil 25and 25water samples )from different origin have been cultured on Ashdown selective agar which gave a higher percentage (36.5%)for B. pseudomallei isolation , 64% was observed in farm soil followed by water samples 48% than cow’s milk33.33% and finally the goat’s milk 26.66%. According to L-arabinose biotyping , 39.72% of the B.pseudomallei isolates were positive for arabinose (Arb+) and 60.3% of isolates were negative for arabinose (Arb-).The virulence testing revealed that most bacterial isolates yield haemolysin, protease, lipase and lecithenase in a percentage of 97.3% , 82.2%, 89.04% and 84.9% respectively.The Arb- biotypes were more virulent than Arb+ biotypes with significant differences (P< 0.05).All bacterial isolates were characterized by their ability to form capsule(100%),.This study confirmed the presence of casein proteolytic and necrotizing enzymes in the suspension of B.pseudomallei .Proteins concentration in this suspension was 0.00248 mg/ml .https://bjvr.uobasrah.edu.iq/article_68925_80809305231e3a4a81f7847000c871a5.pdfgoat milkarabinosesoil |
| spellingShingle | Adnan .M . AL-Rodhan Hanaa K. Ibrahim ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCE Basrah Journal of Veterinary Research goat milk arabinose soil |
| title | ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCE |
| title_full | ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCE |
| title_fullStr | ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCE |
| title_full_unstemmed | ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCE |
| title_short | ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCE |
| title_sort | isolation and identification of burkholderia pseudomallei from cows goat s milk and their surrounding environment in basrah province |
| topic | goat milk arabinose soil |
| url | https://bjvr.uobasrah.edu.iq/article_68925_80809305231e3a4a81f7847000c871a5.pdf |
| work_keys_str_mv | AT adnanmalrodhanhanaakibrahim isolationandidentificationofburkholderiapseudomalleifromcowsgoatsmilkandtheirsurroundingenvironmentinbasrahprovince |