Fabrication of a Polyglycolic Acid Porous Microneedle Array Patch Using the Nonsolvent Induced Phase Separation Method for Body Fluid Extraction

ABSTRACT Traditional blood sampling is essential for early diagnosis and subsequent analysis, but the methods using hypodermic needles are painful and burdensome. Recently, a minimally invasive approach utilizing porous microneedles has been developed and various porous microneedle array patches (MA...

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Main Authors: Heyi Jing, Jongho Park, Beomjoon Kim
Format: Article
Language:English
Published: Wiley-VCH 2025-04-01
Series:Nano Select
Subjects:
Online Access:https://doi.org/10.1002/nano.202400145
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author Heyi Jing
Jongho Park
Beomjoon Kim
author_facet Heyi Jing
Jongho Park
Beomjoon Kim
author_sort Heyi Jing
collection DOAJ
description ABSTRACT Traditional blood sampling is essential for early diagnosis and subsequent analysis, but the methods using hypodermic needles are painful and burdensome. Recently, a minimally invasive approach utilizing porous microneedles has been developed and various porous microneedle array patches (MAPs) composed of biodegradable polymers have been investigated. To address issues about low mechanical strength and liquid absorption with porous MAPs, we used polyglycolic acid (PGA) as it is a biodegradable and hydrophilic polymer with high mechanical properties. In this study, we established a nonsolvent‐induced phase separation (NIPS) method for the fabrication of PGA porous MAPs, as the porous MAPs can be fabricated by simply immersing the molds injected with PGA‐hexafluoro‐2‐propanol (HFIP) solution in nonsolvents. We achieved the maximum liquid absorption rate of 16 ± 8.2 × 10−2 µL/min per one microneedle using the PGA porous MAPs fabricated by using ethanol as nonsolvent and PGA concentration of 10% (w/w). Our study provides a comprehensive understanding of porous MAPs fabricated using PGA material as well as its characteristics regarding the structural and mechanical properties of PGA MAPs, with potential as a diagnostic device to substitute conventional hypodermic needles for interstitial fluid (ISF) sampling and diagnosis.
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spelling doaj-art-b9883abecb5249d98b1dc0d8bf25b8262025-08-20T02:08:45ZengWiley-VCHNano Select2688-40112025-04-0164n/an/a10.1002/nano.202400145Fabrication of a Polyglycolic Acid Porous Microneedle Array Patch Using the Nonsolvent Induced Phase Separation Method for Body Fluid ExtractionHeyi Jing0Jongho Park1Beomjoon Kim2Department of Precision Engineering School of Engineering The University of Tokyo Tokyo JapanInstitute of Industrial Science The University of Tokyo Tokyo JapanInstitute of Industrial Science The University of Tokyo Tokyo JapanABSTRACT Traditional blood sampling is essential for early diagnosis and subsequent analysis, but the methods using hypodermic needles are painful and burdensome. Recently, a minimally invasive approach utilizing porous microneedles has been developed and various porous microneedle array patches (MAPs) composed of biodegradable polymers have been investigated. To address issues about low mechanical strength and liquid absorption with porous MAPs, we used polyglycolic acid (PGA) as it is a biodegradable and hydrophilic polymer with high mechanical properties. In this study, we established a nonsolvent‐induced phase separation (NIPS) method for the fabrication of PGA porous MAPs, as the porous MAPs can be fabricated by simply immersing the molds injected with PGA‐hexafluoro‐2‐propanol (HFIP) solution in nonsolvents. We achieved the maximum liquid absorption rate of 16 ± 8.2 × 10−2 µL/min per one microneedle using the PGA porous MAPs fabricated by using ethanol as nonsolvent and PGA concentration of 10% (w/w). Our study provides a comprehensive understanding of porous MAPs fabricated using PGA material as well as its characteristics regarding the structural and mechanical properties of PGA MAPs, with potential as a diagnostic device to substitute conventional hypodermic needles for interstitial fluid (ISF) sampling and diagnosis.https://doi.org/10.1002/nano.202400145interstitial fluid (ISF)microneedle array patch (MAP)phase separationporous microneedle (MN)sensing
spellingShingle Heyi Jing
Jongho Park
Beomjoon Kim
Fabrication of a Polyglycolic Acid Porous Microneedle Array Patch Using the Nonsolvent Induced Phase Separation Method for Body Fluid Extraction
Nano Select
interstitial fluid (ISF)
microneedle array patch (MAP)
phase separation
porous microneedle (MN)
sensing
title Fabrication of a Polyglycolic Acid Porous Microneedle Array Patch Using the Nonsolvent Induced Phase Separation Method for Body Fluid Extraction
title_full Fabrication of a Polyglycolic Acid Porous Microneedle Array Patch Using the Nonsolvent Induced Phase Separation Method for Body Fluid Extraction
title_fullStr Fabrication of a Polyglycolic Acid Porous Microneedle Array Patch Using the Nonsolvent Induced Phase Separation Method for Body Fluid Extraction
title_full_unstemmed Fabrication of a Polyglycolic Acid Porous Microneedle Array Patch Using the Nonsolvent Induced Phase Separation Method for Body Fluid Extraction
title_short Fabrication of a Polyglycolic Acid Porous Microneedle Array Patch Using the Nonsolvent Induced Phase Separation Method for Body Fluid Extraction
title_sort fabrication of a polyglycolic acid porous microneedle array patch using the nonsolvent induced phase separation method for body fluid extraction
topic interstitial fluid (ISF)
microneedle array patch (MAP)
phase separation
porous microneedle (MN)
sensing
url https://doi.org/10.1002/nano.202400145
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AT jonghopark fabricationofapolyglycolicacidporousmicroneedlearraypatchusingthenonsolventinducedphaseseparationmethodforbodyfluidextraction
AT beomjoonkim fabricationofapolyglycolicacidporousmicroneedlearraypatchusingthenonsolventinducedphaseseparationmethodforbodyfluidextraction