Development of a Specific PCR Assay for <i>Theileria</i> sp. Yokoyama and Assessment of Its Potential to Cause Anemia in Cattle
The clinical implications of <i>Theileria</i> sp. Yokoyama, a recently identified <i>Theileria</i> species in cattle, remain uncertain. The objective of the present study was to evaluate the anemia status in cattle infected with <i>Theileria</i> sp. Yokoyama. Bloo...
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MDPI AG
2024-08-01
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| author | Iromy Dhananjani Amarasiri Kalaichelvan Nizanantha Ngigi Noel Muthoni Mumbi Isuru Sachintha Kothalawala Sampath Madusanka Wettam Perumage Pavithra Sandamali Indrasiri Perera Hemal Kothalawala Thillaiampalam Sivakumar Naoaki Yokoyama |
| author_facet | Iromy Dhananjani Amarasiri Kalaichelvan Nizanantha Ngigi Noel Muthoni Mumbi Isuru Sachintha Kothalawala Sampath Madusanka Wettam Perumage Pavithra Sandamali Indrasiri Perera Hemal Kothalawala Thillaiampalam Sivakumar Naoaki Yokoyama |
| author_sort | Iromy Dhananjani Amarasiri |
| collection | DOAJ |
| description | The clinical implications of <i>Theileria</i> sp. Yokoyama, a recently identified <i>Theileria</i> species in cattle, remain uncertain. The objective of the present study was to evaluate the anemia status in cattle infected with <i>Theileria</i> sp. Yokoyama. Blood samples were collected from 206 cattle across seven Veterinary Ranges in Sri Lanka and analyzed for red blood cell (RBC) indices, including hemoglobin concentration, hematocrit, and RBC counts. Additionally, DNA was extracted from the samples and screened with a newly developed <i>Theileria</i> sp. Yokoyama-specific PCR assay targeting the cytochrome b gene. The PCR results revealed that 60 (29.1%) of the surveyed cattle tested positive for <i>Theileria</i> sp. Yokoyama, with 47 (78.3%) of them being co-infected with other hemopathogen species. Our findings revealed that the cattle breeds, management systems, and tick infestations are potential risk factors for the <i>Theileria</i> sp. Yokoyama infection. Next, we evaluated the anemia status among the surveyed cattle based on the RBC indices. We found that all non-infected cattle were non-anemic. By contrast, anemia was observed in 15 <i>Theileria</i> sp. Yokoyama-infected cattle, including 3 singly infected (anemia rate 3/13, 23.1%) and 12 co-infected cattle (12/47, 25.5%). Our findings suggest that <i>Theileria</i> sp. Yokoyama causes anemia in infected cattle. |
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| id | doaj-art-b97419188b7c4f00a0a46d7d2b300751 |
| institution | OA Journals |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-08-01 |
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| spelling | doaj-art-b97419188b7c4f00a0a46d7d2b3007512025-08-20T01:55:45ZengMDPI AGPathogens2076-08172024-08-0113973510.3390/pathogens13090735Development of a Specific PCR Assay for <i>Theileria</i> sp. Yokoyama and Assessment of Its Potential to Cause Anemia in CattleIromy Dhananjani Amarasiri0Kalaichelvan Nizanantha1Ngigi Noel Muthoni Mumbi2Isuru Sachintha Kothalawala3Sampath Madusanka4Wettam Perumage Pavithra Sandamali Indrasiri Perera5Hemal Kothalawala6Thillaiampalam Sivakumar7Naoaki Yokoyama8Veterinary Research Institute, Peradeniya 20400, Sri LankaDepartment of Farm Animal Production and Health, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya 20400, Sri LankaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro 080-8555, Hokkaido, JapanDepartment of Biochemistry, Faculty of Medicine, University of Peradeniya, Peradeniya 20400, Sri LankaNational Livestock Development Board, No. 40, Nawala Road, Narahenpita, Colombo 00500, Sri LankaDepartment of Farm Animal Production and Health, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya 20400, Sri LankaVeterinary Research Institute, Peradeniya 20400, Sri LankaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro 080-8555, Hokkaido, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro 080-8555, Hokkaido, JapanThe clinical implications of <i>Theileria</i> sp. Yokoyama, a recently identified <i>Theileria</i> species in cattle, remain uncertain. The objective of the present study was to evaluate the anemia status in cattle infected with <i>Theileria</i> sp. Yokoyama. Blood samples were collected from 206 cattle across seven Veterinary Ranges in Sri Lanka and analyzed for red blood cell (RBC) indices, including hemoglobin concentration, hematocrit, and RBC counts. Additionally, DNA was extracted from the samples and screened with a newly developed <i>Theileria</i> sp. Yokoyama-specific PCR assay targeting the cytochrome b gene. The PCR results revealed that 60 (29.1%) of the surveyed cattle tested positive for <i>Theileria</i> sp. Yokoyama, with 47 (78.3%) of them being co-infected with other hemopathogen species. Our findings revealed that the cattle breeds, management systems, and tick infestations are potential risk factors for the <i>Theileria</i> sp. Yokoyama infection. Next, we evaluated the anemia status among the surveyed cattle based on the RBC indices. We found that all non-infected cattle were non-anemic. By contrast, anemia was observed in 15 <i>Theileria</i> sp. Yokoyama-infected cattle, including 3 singly infected (anemia rate 3/13, 23.1%) and 12 co-infected cattle (12/47, 25.5%). Our findings suggest that <i>Theileria</i> sp. Yokoyama causes anemia in infected cattle.https://www.mdpi.com/2076-0817/13/9/735anemiacattlePCR<i>Theileria</i> sp. Yokoyama |
| spellingShingle | Iromy Dhananjani Amarasiri Kalaichelvan Nizanantha Ngigi Noel Muthoni Mumbi Isuru Sachintha Kothalawala Sampath Madusanka Wettam Perumage Pavithra Sandamali Indrasiri Perera Hemal Kothalawala Thillaiampalam Sivakumar Naoaki Yokoyama Development of a Specific PCR Assay for <i>Theileria</i> sp. Yokoyama and Assessment of Its Potential to Cause Anemia in Cattle Pathogens anemia cattle PCR <i>Theileria</i> sp. Yokoyama |
| title | Development of a Specific PCR Assay for <i>Theileria</i> sp. Yokoyama and Assessment of Its Potential to Cause Anemia in Cattle |
| title_full | Development of a Specific PCR Assay for <i>Theileria</i> sp. Yokoyama and Assessment of Its Potential to Cause Anemia in Cattle |
| title_fullStr | Development of a Specific PCR Assay for <i>Theileria</i> sp. Yokoyama and Assessment of Its Potential to Cause Anemia in Cattle |
| title_full_unstemmed | Development of a Specific PCR Assay for <i>Theileria</i> sp. Yokoyama and Assessment of Its Potential to Cause Anemia in Cattle |
| title_short | Development of a Specific PCR Assay for <i>Theileria</i> sp. Yokoyama and Assessment of Its Potential to Cause Anemia in Cattle |
| title_sort | development of a specific pcr assay for i theileria i sp yokoyama and assessment of its potential to cause anemia in cattle |
| topic | anemia cattle PCR <i>Theileria</i> sp. Yokoyama |
| url | https://www.mdpi.com/2076-0817/13/9/735 |
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