CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation

Background. The therapeutic efficacy of allogeneic hemopoietic stem cell transplantation (HSCT) largely relies on the graft-versus-leukemia (GVL) effect. Uncontrolled graft-versus-host disease (GVHD) is a feared complication of HSCT. Regulatory T cells (Treg) are a subset of CD4+ T-helper cells beli...

Full description

Saved in:
Bibliographic Details
Main Authors: Jolanta B. Perz, Selma Gürel, Stefan O. Schonland, Ute Hegenbart, Anthony D. Ho, Peter Dreger
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/606839
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832545600453738496
author Jolanta B. Perz
Selma Gürel
Stefan O. Schonland
Ute Hegenbart
Anthony D. Ho
Peter Dreger
author_facet Jolanta B. Perz
Selma Gürel
Stefan O. Schonland
Ute Hegenbart
Anthony D. Ho
Peter Dreger
author_sort Jolanta B. Perz
collection DOAJ
description Background. The therapeutic efficacy of allogeneic hemopoietic stem cell transplantation (HSCT) largely relies on the graft-versus-leukemia (GVL) effect. Uncontrolled graft-versus-host disease (GVHD) is a feared complication of HSCT. Regulatory T cells (Treg) are a subset of CD4+ T-helper cells believed to maintain tolerance after HSCT. It remains unclear whether low peripheral blood Treg have an impact on the risk for acute (aGVHD) and chronic GVHD (cGVHD). Methods. In this paper we enumerated the CD4+CD25highCD127low Treg in the peripheral blood of 84 patients after at least 150 days from HSCT and in 20 healthy age-matched controls. Results. Although similar mean lymphocyte counts were found in patients and controls, CD3+CD4+ T-cell counts were significantly lower in patients. Patients also had significantly lower Treg percentages among lymphocytes as compared to controls. Patients with cGVHD had even higher percentages of Treg if compared to patients without cGVHD. In multivariate analysis, Treg percentages were not an independent factor for cGVHD. Conclusions. This paper did not show a relation between deficient peripheral blood Treg and cGVHD, therefore cGVHD does not seem to occur as a result of peripheral Treg paucity.
format Article
id doaj-art-b96f7012e2c34bc9abe46142d9fdaa8f
institution Kabale University
issn 1537-744X
language English
publishDate 2012-01-01
publisher Wiley
record_format Article
series The Scientific World Journal
spelling doaj-art-b96f7012e2c34bc9abe46142d9fdaa8f2025-02-03T07:25:27ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/606839606839CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell TransplantationJolanta B. Perz0Selma Gürel1Stefan O. Schonland2Ute Hegenbart3Anthony D. Ho4Peter Dreger5Department of Internal Medicine-Hematology, Oncology and Rheumatology, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Internal Medicine-Hematology, Oncology and Rheumatology, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Internal Medicine-Hematology, Oncology and Rheumatology, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Internal Medicine-Hematology, Oncology and Rheumatology, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Internal Medicine-Hematology, Oncology and Rheumatology, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Internal Medicine-Hematology, Oncology and Rheumatology, University Hospital Heidelberg, 69120 Heidelberg, GermanyBackground. The therapeutic efficacy of allogeneic hemopoietic stem cell transplantation (HSCT) largely relies on the graft-versus-leukemia (GVL) effect. Uncontrolled graft-versus-host disease (GVHD) is a feared complication of HSCT. Regulatory T cells (Treg) are a subset of CD4+ T-helper cells believed to maintain tolerance after HSCT. It remains unclear whether low peripheral blood Treg have an impact on the risk for acute (aGVHD) and chronic GVHD (cGVHD). Methods. In this paper we enumerated the CD4+CD25highCD127low Treg in the peripheral blood of 84 patients after at least 150 days from HSCT and in 20 healthy age-matched controls. Results. Although similar mean lymphocyte counts were found in patients and controls, CD3+CD4+ T-cell counts were significantly lower in patients. Patients also had significantly lower Treg percentages among lymphocytes as compared to controls. Patients with cGVHD had even higher percentages of Treg if compared to patients without cGVHD. In multivariate analysis, Treg percentages were not an independent factor for cGVHD. Conclusions. This paper did not show a relation between deficient peripheral blood Treg and cGVHD, therefore cGVHD does not seem to occur as a result of peripheral Treg paucity.http://dx.doi.org/10.1100/2012/606839
spellingShingle Jolanta B. Perz
Selma Gürel
Stefan O. Schonland
Ute Hegenbart
Anthony D. Ho
Peter Dreger
CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation
The Scientific World Journal
title CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation
title_full CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation
title_fullStr CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation
title_full_unstemmed CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation
title_short CD4+CD25highCD127low Regulatory T Cells in Peripheral Blood Are Not an Independent Factor for Chronic Graft-versus-Host Disease after Allogeneic Stem Cell Transplantation
title_sort cd4 cd25highcd127low regulatory t cells in peripheral blood are not an independent factor for chronic graft versus host disease after allogeneic stem cell transplantation
url http://dx.doi.org/10.1100/2012/606839
work_keys_str_mv AT jolantabperz cd4cd25highcd127lowregulatorytcellsinperipheralbloodarenotanindependentfactorforchronicgraftversushostdiseaseafterallogeneicstemcelltransplantation
AT selmagurel cd4cd25highcd127lowregulatorytcellsinperipheralbloodarenotanindependentfactorforchronicgraftversushostdiseaseafterallogeneicstemcelltransplantation
AT stefanoschonland cd4cd25highcd127lowregulatorytcellsinperipheralbloodarenotanindependentfactorforchronicgraftversushostdiseaseafterallogeneicstemcelltransplantation
AT utehegenbart cd4cd25highcd127lowregulatorytcellsinperipheralbloodarenotanindependentfactorforchronicgraftversushostdiseaseafterallogeneicstemcelltransplantation
AT anthonydho cd4cd25highcd127lowregulatorytcellsinperipheralbloodarenotanindependentfactorforchronicgraftversushostdiseaseafterallogeneicstemcelltransplantation
AT peterdreger cd4cd25highcd127lowregulatorytcellsinperipheralbloodarenotanindependentfactorforchronicgraftversushostdiseaseafterallogeneicstemcelltransplantation