Korean Red Ginseng Saponin Fraction Rich in Ginsenoside-Rb1, Rc and Rb2 Attenuates the Severity of Mouse Collagen-Induced Arthritis
Despite a multitude of reports on anti-inflammatory properties of ginseng extracts or individual ginsenosides, data on antiarthritic effect of ginseng saponin preparation with mixed ginsenosides is limited. On the other hand, a combined therapy of safe and inexpensive plant-derived natural products...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/748964 |
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| author | Mehari Endale Eun Ju Im Joo Young Lee Sung Dae Kim Taddesse Yayeh Yong-Bum Song Yi-Seong Kwak Chaekyun Kim Seung-Hyung Kim Seong-Soo Roh Jae Youl Cho Man Hee Rhee |
| author_facet | Mehari Endale Eun Ju Im Joo Young Lee Sung Dae Kim Taddesse Yayeh Yong-Bum Song Yi-Seong Kwak Chaekyun Kim Seung-Hyung Kim Seong-Soo Roh Jae Youl Cho Man Hee Rhee |
| author_sort | Mehari Endale |
| collection | DOAJ |
| description | Despite a multitude of reports on anti-inflammatory properties of ginseng extracts or individual ginsenosides, data on antiarthritic effect of ginseng saponin preparation with mixed ginsenosides is limited. On the other hand, a combined therapy of safe and inexpensive plant-derived natural products such as ginsenosides can be considered as an alternative to treat arthritis. Our previous in vitro data displayed a strong anti-inflammatory action of red ginseng saponin fraction-A (RGSF-A). We, herein, report a marked antiarthritic property of RGSF-A rich in ginsenoside Rb1, Rc, and Rb2. Collagen-induced arthritic (CIA) mice were treated with RGSF-A or methotrexate (MTX) for 5 weeks. Joint pathology, serum antibody production and leukocye activation, cytokine production in the circulation, lymph nodes, and joints were examined. RGSF-A markedly reduced severity of arthritis, cellular infiltration, and cartilage damage. It suppressed CD3+/CD69+, CD4+/CD25+, CD8+ T-cell, CD19+, B220/CD23+ B-cell, MHCII+/CD11c+, and Gr-1+/CD11b+ cell activations. It further suppressed anti-CII- or anti-RF-IgG/IgM, TNF-α, IL-1β, IL-17, and IL-6 secretions but stimulated IL-10 levels in the serum, joint, or splenocyte. RGSF-A attenuated arthritis severity, modified leukocyte activations, and restored cytokine imbalances, suggesting that it can be considered as an antiarthritic agent with the capacity to ameliorate the immune and inflammatory responses in CIA mice. |
| format | Article |
| id | doaj-art-b958cfe5f90944a8a25d8dda192037e8 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b958cfe5f90944a8a25d8dda192037e82025-08-20T03:23:14ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/748964748964Korean Red Ginseng Saponin Fraction Rich in Ginsenoside-Rb1, Rc and Rb2 Attenuates the Severity of Mouse Collagen-Induced ArthritisMehari Endale0Eun Ju Im1Joo Young Lee2Sung Dae Kim3Taddesse Yayeh4Yong-Bum Song5Yi-Seong Kwak6Chaekyun Kim7Seung-Hyung Kim8Seong-Soo Roh9Jae Youl Cho10Man Hee Rhee11Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of KoreaLaboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of KoreaLaboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of KoreaResearch Center, Dongnam Institute of Radiological & Medical Sciences, Busan 619-953, Republic of KoreaDebre Markos University, Debre Markos, EthiopiaGinseng Corporation Central Research Institute, Daejeon 305-805, Republic of KoreaGinseng Corporation Central Research Institute, Daejeon 305-805, Republic of KoreaDepartment of Pharmacology, Inha University School of Medicine, Incheon 400-712, Republic of KoreaInstitute of Traditional Medicine & Bioscience, Daejeon University, Daejeon 300-716, Republic of KoreaDepartment of Herbology, College of Korean Medicine, Daegu Hanny University, Gyeongsan 712-715, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of KoreaLaboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of KoreaDespite a multitude of reports on anti-inflammatory properties of ginseng extracts or individual ginsenosides, data on antiarthritic effect of ginseng saponin preparation with mixed ginsenosides is limited. On the other hand, a combined therapy of safe and inexpensive plant-derived natural products such as ginsenosides can be considered as an alternative to treat arthritis. Our previous in vitro data displayed a strong anti-inflammatory action of red ginseng saponin fraction-A (RGSF-A). We, herein, report a marked antiarthritic property of RGSF-A rich in ginsenoside Rb1, Rc, and Rb2. Collagen-induced arthritic (CIA) mice were treated with RGSF-A or methotrexate (MTX) for 5 weeks. Joint pathology, serum antibody production and leukocye activation, cytokine production in the circulation, lymph nodes, and joints were examined. RGSF-A markedly reduced severity of arthritis, cellular infiltration, and cartilage damage. It suppressed CD3+/CD69+, CD4+/CD25+, CD8+ T-cell, CD19+, B220/CD23+ B-cell, MHCII+/CD11c+, and Gr-1+/CD11b+ cell activations. It further suppressed anti-CII- or anti-RF-IgG/IgM, TNF-α, IL-1β, IL-17, and IL-6 secretions but stimulated IL-10 levels in the serum, joint, or splenocyte. RGSF-A attenuated arthritis severity, modified leukocyte activations, and restored cytokine imbalances, suggesting that it can be considered as an antiarthritic agent with the capacity to ameliorate the immune and inflammatory responses in CIA mice.http://dx.doi.org/10.1155/2014/748964 |
| spellingShingle | Mehari Endale Eun Ju Im Joo Young Lee Sung Dae Kim Taddesse Yayeh Yong-Bum Song Yi-Seong Kwak Chaekyun Kim Seung-Hyung Kim Seong-Soo Roh Jae Youl Cho Man Hee Rhee Korean Red Ginseng Saponin Fraction Rich in Ginsenoside-Rb1, Rc and Rb2 Attenuates the Severity of Mouse Collagen-Induced Arthritis Mediators of Inflammation |
| title | Korean Red Ginseng Saponin Fraction Rich in Ginsenoside-Rb1, Rc and Rb2 Attenuates the Severity of Mouse Collagen-Induced Arthritis |
| title_full | Korean Red Ginseng Saponin Fraction Rich in Ginsenoside-Rb1, Rc and Rb2 Attenuates the Severity of Mouse Collagen-Induced Arthritis |
| title_fullStr | Korean Red Ginseng Saponin Fraction Rich in Ginsenoside-Rb1, Rc and Rb2 Attenuates the Severity of Mouse Collagen-Induced Arthritis |
| title_full_unstemmed | Korean Red Ginseng Saponin Fraction Rich in Ginsenoside-Rb1, Rc and Rb2 Attenuates the Severity of Mouse Collagen-Induced Arthritis |
| title_short | Korean Red Ginseng Saponin Fraction Rich in Ginsenoside-Rb1, Rc and Rb2 Attenuates the Severity of Mouse Collagen-Induced Arthritis |
| title_sort | korean red ginseng saponin fraction rich in ginsenoside rb1 rc and rb2 attenuates the severity of mouse collagen induced arthritis |
| url | http://dx.doi.org/10.1155/2014/748964 |
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