Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro
Southern catfish (Silurus meridionalis) is widely distributed in the Yangtze River and its tributaries and is valued for its rapid growth, nutritional content, pleasant taste, absence of intermuscular bone, and disease resistance. However, the species faces challenges in genetic breeding and germ-li...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006756 |
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| author | Jianeng Li Changle Zhao Lei Liu Shuqing Zheng Deshou Wang Changwei Shao Jing Wei |
| author_facet | Jianeng Li Changle Zhao Lei Liu Shuqing Zheng Deshou Wang Changwei Shao Jing Wei |
| author_sort | Jianeng Li |
| collection | DOAJ |
| description | Southern catfish (Silurus meridionalis) is widely distributed in the Yangtze River and its tributaries and is valued for its rapid growth, nutritional content, pleasant taste, absence of intermuscular bone, and disease resistance. However, the species faces challenges in genetic breeding and germ-line preservation due to its late sexual maturity (2–3 years for males and 3–4 years for females) and single spawning cycle. In this study, we successfully established a spermatogonial stem cell line (designated as SC1) derived from the testis of a four-month-old Southern catfish. This cell line has exhibited continuous growth for more than one year, showing significant alkaline phosphatase activity, and maintaining a diploid karyotype. It exhibited expression of germ cell markers, including glial cell-derived neurotrophic factor family receptor alpha-1 (gfrα1) a and b, pou5f3, nanog, and vasa, but no expression of somatic cell markers such as amh, wt1a, and wt1b. Notably, SC1 has shown the ability to differentiate into sperm-like cells in vitro following treatment with retinoic acid (RA) and/or 11-ketotestosterone (KT). Transfection efficiency exceeding 50 % was achieved utilizing microporous membrane electroporation. Furthermore, the sustained proliferation of SC1 was found to depend on a combination of various factors, particularly the extract from Nile tilapia embryos. Collectively, our findings indicate the successful establishment of a Southern catfish spermatogonial stem cell line that exhibits sustained proliferation and sperm production in vitro. This study provides a foundation for generating genome-edited sperm for germ-line transmission in this species and offers a valuable tool for investigating fish spermatogenesis in vitro. |
| format | Article |
| id | doaj-art-b955b43abbd3478c87c46eedb9406b41 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-b955b43abbd3478c87c46eedb9406b412025-02-06T05:12:13ZengElsevierAquaculture Reports2352-51342025-03-0140102587Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitroJianeng Li0Changle Zhao1Lei Liu2Shuqing Zheng3Deshou Wang4Changwei Shao5Jing Wei6National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China; Corresponding authors at: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Corresponding author.National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, Chongqing 400715, China; Corresponding authors at: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.Southern catfish (Silurus meridionalis) is widely distributed in the Yangtze River and its tributaries and is valued for its rapid growth, nutritional content, pleasant taste, absence of intermuscular bone, and disease resistance. However, the species faces challenges in genetic breeding and germ-line preservation due to its late sexual maturity (2–3 years for males and 3–4 years for females) and single spawning cycle. In this study, we successfully established a spermatogonial stem cell line (designated as SC1) derived from the testis of a four-month-old Southern catfish. This cell line has exhibited continuous growth for more than one year, showing significant alkaline phosphatase activity, and maintaining a diploid karyotype. It exhibited expression of germ cell markers, including glial cell-derived neurotrophic factor family receptor alpha-1 (gfrα1) a and b, pou5f3, nanog, and vasa, but no expression of somatic cell markers such as amh, wt1a, and wt1b. Notably, SC1 has shown the ability to differentiate into sperm-like cells in vitro following treatment with retinoic acid (RA) and/or 11-ketotestosterone (KT). Transfection efficiency exceeding 50 % was achieved utilizing microporous membrane electroporation. Furthermore, the sustained proliferation of SC1 was found to depend on a combination of various factors, particularly the extract from Nile tilapia embryos. Collectively, our findings indicate the successful establishment of a Southern catfish spermatogonial stem cell line that exhibits sustained proliferation and sperm production in vitro. This study provides a foundation for generating genome-edited sperm for germ-line transmission in this species and offers a valuable tool for investigating fish spermatogenesis in vitro.http://www.sciencedirect.com/science/article/pii/S2352513424006756CatfishSpermatogonial stem cell lineSpermSpermatogenesis in vitro |
| spellingShingle | Jianeng Li Changle Zhao Lei Liu Shuqing Zheng Deshou Wang Changwei Shao Jing Wei Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro Aquaculture Reports Catfish Spermatogonial stem cell line Sperm Spermatogenesis in vitro |
| title | Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro |
| title_full | Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro |
| title_fullStr | Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro |
| title_full_unstemmed | Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro |
| title_short | Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro |
| title_sort | establishment of a southern catfish silurus meridionalis spermatogonial stem cell line capable of sperm production in vitro |
| topic | Catfish Spermatogonial stem cell line Sperm Spermatogenesis in vitro |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006756 |
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