Ordered assemblies of peptide nanoparticles with only positive charge

Abstract Surface charge patchiness of different charge types can influence the solution behaviours of colloidal particles and globular proteins. Herein, coiled-coil ‘bundlemer’ nanoparticles that display only a single type of surface charge (SC) are computationally designed to compare their solution...

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Main Authors: Yi Shi, Tianren Zhang, Rui Guo, Zihan Zhang, Amanda L. McCahill, Yao Tang, Sabrina E. Liskey, Dai-Bei Yang, Christopher J. Kloxin, Jeffery G. Saven, Darrin J. Pochan
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-54340-9
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author Yi Shi
Tianren Zhang
Rui Guo
Zihan Zhang
Amanda L. McCahill
Yao Tang
Sabrina E. Liskey
Dai-Bei Yang
Christopher J. Kloxin
Jeffery G. Saven
Darrin J. Pochan
author_facet Yi Shi
Tianren Zhang
Rui Guo
Zihan Zhang
Amanda L. McCahill
Yao Tang
Sabrina E. Liskey
Dai-Bei Yang
Christopher J. Kloxin
Jeffery G. Saven
Darrin J. Pochan
author_sort Yi Shi
collection DOAJ
description Abstract Surface charge patchiness of different charge types can influence the solution behaviours of colloidal particles and globular proteins. Herein, coiled-coil ‘bundlemer’ nanoparticles that display only a single type of surface charge (SC) are computationally designed to compare their solution behaviours to mixed charge-type (MC) counterparts with both positively and negatively charged side chains. Nematic and columnar liquid crystal phases are discovered in low concentrations of SC particles, indicative of particle end-to-end stacking into columns combined with lateral electrostatic repulsion between columns, while MC particles with the same net charge and particle shape produced only amorphous, soluble aggregates. Similarly, porous lattices are formed in mixtures of SC/MC particles of opposite charges while MC/MC mixtures of opposite charges produce only amorphous aggregates. The lattice structure is inferred with a machine learning optimization approach. The differences between SC and MC particle behaviours directly show the importance of surface electrostatic patchiness.
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series Nature Communications
spelling doaj-art-b94d0f201150413c91aa62fbc09c883f2025-08-20T02:33:06ZengNature PortfolioNature Communications2041-17232024-11-0115111110.1038/s41467-024-54340-9Ordered assemblies of peptide nanoparticles with only positive chargeYi Shi0Tianren Zhang1Rui Guo2Zihan Zhang3Amanda L. McCahill4Yao Tang5Sabrina E. Liskey6Dai-Bei Yang7Christopher J. Kloxin8Jeffery G. Saven9Darrin J. Pochan10Department of Materials Science and Engineering, University of DelawareDepartment of Materials Science and Engineering, University of DelawareDepartment of Chemistry, University of PennsylvaniaDepartment of Materials Science and Engineering, University of DelawareDepartment of Materials Science and Engineering, University of DelawareDepartment of Materials Science and Engineering, University of DelawareDepartment of Materials Science and Engineering, University of DelawareDepartment of Chemistry, University of PennsylvaniaDepartment of Materials Science and Engineering, University of DelawareDepartment of Chemistry, University of PennsylvaniaDepartment of Materials Science and Engineering, University of DelawareAbstract Surface charge patchiness of different charge types can influence the solution behaviours of colloidal particles and globular proteins. Herein, coiled-coil ‘bundlemer’ nanoparticles that display only a single type of surface charge (SC) are computationally designed to compare their solution behaviours to mixed charge-type (MC) counterparts with both positively and negatively charged side chains. Nematic and columnar liquid crystal phases are discovered in low concentrations of SC particles, indicative of particle end-to-end stacking into columns combined with lateral electrostatic repulsion between columns, while MC particles with the same net charge and particle shape produced only amorphous, soluble aggregates. Similarly, porous lattices are formed in mixtures of SC/MC particles of opposite charges while MC/MC mixtures of opposite charges produce only amorphous aggregates. The lattice structure is inferred with a machine learning optimization approach. The differences between SC and MC particle behaviours directly show the importance of surface electrostatic patchiness.https://doi.org/10.1038/s41467-024-54340-9
spellingShingle Yi Shi
Tianren Zhang
Rui Guo
Zihan Zhang
Amanda L. McCahill
Yao Tang
Sabrina E. Liskey
Dai-Bei Yang
Christopher J. Kloxin
Jeffery G. Saven
Darrin J. Pochan
Ordered assemblies of peptide nanoparticles with only positive charge
Nature Communications
title Ordered assemblies of peptide nanoparticles with only positive charge
title_full Ordered assemblies of peptide nanoparticles with only positive charge
title_fullStr Ordered assemblies of peptide nanoparticles with only positive charge
title_full_unstemmed Ordered assemblies of peptide nanoparticles with only positive charge
title_short Ordered assemblies of peptide nanoparticles with only positive charge
title_sort ordered assemblies of peptide nanoparticles with only positive charge
url https://doi.org/10.1038/s41467-024-54340-9
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