Development of a green, efficient and renewable biorefinery strategy for the production of xylooligosaccharides from corncobs based on a novel specific xylanase
For the efficient bio‒production of xylooligosaccharides (XOSs) from corncobs, a novel acidophilic and thermostable xylanase from Thermoascus aurantiacus was identified and characterized. The xylanase gene (TaXyn929) was heterologously expressed in Pichia pastoris, and the highest xylanase activity...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001963 |
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| Summary: | For the efficient bio‒production of xylooligosaccharides (XOSs) from corncobs, a novel acidophilic and thermostable xylanase from Thermoascus aurantiacus was identified and characterized. The xylanase gene (TaXyn929) was heterologously expressed in Pichia pastoris, and the highest xylanase activity of 5, 896 U/mL was achieved in a 5 L fermenter by high‒cell density fermentation. The recombinant enzyme (TaXyn929) was most active at pH 5.0 and 75 °C, respectively. It exhibited strict substrate specificity on xylans, showing the highest specific activity of 3, 381 U/mg towards arabinoxylan. On the basis of the newly identified enzyme, a strategy consisting of malic acid pretreatment and enzymatic hydrolysis was developed for the bio‒production of XOSs from corncobs. The highest XOSs yield of 56.9 % (w/w) was obtained under the optimized reaction conditions. In the procedure, the used malic acids (64.2 %, w/w) were offered by the fermentation of A. oryzae using glucoses as the carbon source, which were converted by the hydrolysis of corncob debris (82 %, w/w) remained in the first stage via a synergistic reaction of cellulose and β‒glucosidase. This study may provide a novel specific arabinoxylanase as well as a new green route for bio‒refining of lignocellulose. |
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| ISSN: | 2666-8939 |