Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines
Fulvestrant (ICI 182,780; ICI) is approved for the treatment of advanced metastatic breast cancer that is unresponsive to other endocrine therapies. Berries are frequently consumed for their antioxidant, anti-inflammatory, and anticancer potential. In this study, we tested the efficacy of two berry...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Breast Cancer |
| Online Access: | http://dx.doi.org/10.1155/2012/147828 |
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| author | Denzel R. Woode Harini S. Aiyer Nicole Sie Alan L. Zwart Liya Li Navindra P. Seeram Robert Clarke |
| author_facet | Denzel R. Woode Harini S. Aiyer Nicole Sie Alan L. Zwart Liya Li Navindra P. Seeram Robert Clarke |
| author_sort | Denzel R. Woode |
| collection | DOAJ |
| description | Fulvestrant (ICI 182,780; ICI) is approved for the treatment of advanced metastatic breast cancer that is unresponsive to other endocrine therapies. Berries are frequently consumed for their antioxidant, anti-inflammatory, and anticancer potential. In this study, we tested the efficacy of two berry extracts (Jamun-EJAE and red raspberry-RRE) and their bioactive compounds (Delphinidin-Del and Ellagic acid-EA) to inhibit cell proliferation with or without a sublethal dose of ICI in various breast cancer cell lines. ICI-sensitive (LCC1, ZR75-1, and BT474) and -resistant (LCC9, ZR75-1R) cells were subjected to treatment with berry extracts alone (0.1–100 μg/mL) or with a sub-lethal dose of ICI (<IC50 dose; 1 nM for sensitive; 1 μM for resistant cells). Extracts and Del enhanced the effect of ICI in sensitive ZR75-1 and BT474 cells primarily in an additive fashion (measured by relative index (RI)~1). In ZR75-1R cells, both EJAE and RRE synergistically enhanced the effects of ICI (15–50%; ; ). EA, in doses tested, did not have any significant effects on any of the cell lines. Finally, we found that the extracts were more effective at lower, physiologically relevant concentrations than at higher experimental doses. |
| format | Article |
| id | doaj-art-b948e5211ce7405d8c83d485f0beabfe |
| institution | DOAJ |
| issn | 2090-3170 2090-3189 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Breast Cancer |
| spelling | doaj-art-b948e5211ce7405d8c83d485f0beabfe2025-08-20T03:23:12ZengWileyInternational Journal of Breast Cancer2090-31702090-31892012-01-01201210.1155/2012/147828147828Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell LinesDenzel R. Woode0Harini S. Aiyer1Nicole Sie2Alan L. Zwart3Liya Li4Navindra P. Seeram5Robert Clarke6Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USALombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USARoyal College of Surgeons in Ireland, Dublin, IrelandLombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USADepartment of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 7 Greenhouse Road, Kingston, RI 02881, USADepartment of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 7 Greenhouse Road, Kingston, RI 02881, USALombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USAFulvestrant (ICI 182,780; ICI) is approved for the treatment of advanced metastatic breast cancer that is unresponsive to other endocrine therapies. Berries are frequently consumed for their antioxidant, anti-inflammatory, and anticancer potential. In this study, we tested the efficacy of two berry extracts (Jamun-EJAE and red raspberry-RRE) and their bioactive compounds (Delphinidin-Del and Ellagic acid-EA) to inhibit cell proliferation with or without a sublethal dose of ICI in various breast cancer cell lines. ICI-sensitive (LCC1, ZR75-1, and BT474) and -resistant (LCC9, ZR75-1R) cells were subjected to treatment with berry extracts alone (0.1–100 μg/mL) or with a sub-lethal dose of ICI (<IC50 dose; 1 nM for sensitive; 1 μM for resistant cells). Extracts and Del enhanced the effect of ICI in sensitive ZR75-1 and BT474 cells primarily in an additive fashion (measured by relative index (RI)~1). In ZR75-1R cells, both EJAE and RRE synergistically enhanced the effects of ICI (15–50%; ; ). EA, in doses tested, did not have any significant effects on any of the cell lines. Finally, we found that the extracts were more effective at lower, physiologically relevant concentrations than at higher experimental doses.http://dx.doi.org/10.1155/2012/147828 |
| spellingShingle | Denzel R. Woode Harini S. Aiyer Nicole Sie Alan L. Zwart Liya Li Navindra P. Seeram Robert Clarke Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines International Journal of Breast Cancer |
| title | Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines |
| title_full | Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines |
| title_fullStr | Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines |
| title_full_unstemmed | Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines |
| title_short | Effect of Berry Extracts and Bioactive Compounds on Fulvestrant (ICI 182,780) Sensitive and Resistant Cell Lines |
| title_sort | effect of berry extracts and bioactive compounds on fulvestrant ici 182 780 sensitive and resistant cell lines |
| url | http://dx.doi.org/10.1155/2012/147828 |
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