Molecular Elucidation of Anthocyanin Accumulation Mechanisms in <i>Hippeastrum hybridum</i> Cultivars
<i>Hippeastrum</i>, a perennial herbaceous plant belonging to the <i>Amaryllidaceae</i> family, is widely cultivated for its large, vibrant flowers with diverse petal colors, which have significant ornamental and economic value. However, the mechanisms underlying anthocyanin...
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2025-07-01
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| author | Pengyu Guo Chuanji Xing Jiacheng Ye Jing Xue Luis A. J. Mur Bao Di Zongli Hu Guoping Chen Xiuhai Zhang Xuqing Chen |
| author_facet | Pengyu Guo Chuanji Xing Jiacheng Ye Jing Xue Luis A. J. Mur Bao Di Zongli Hu Guoping Chen Xiuhai Zhang Xuqing Chen |
| author_sort | Pengyu Guo |
| collection | DOAJ |
| description | <i>Hippeastrum</i>, a perennial herbaceous plant belonging to the <i>Amaryllidaceae</i> family, is widely cultivated for its large, vibrant flowers with diverse petal colors, which have significant ornamental and economic value. However, the mechanisms underlying anthocyanin accumulation in <i>Hippeastrum</i> petals remain poorly understood. To fully explore the involved regulation mechanism was significant for the breeding of <i>Hippeastrum</i> and other <i>Amaryllidaceae</i> family plants. In this study, we selected six <i>Hippeastrum</i> cultivars with distinctly different petal colors. We used metabolomic profiling and high-throughput transcriptomic sequencing to assess varied anthocyanin profiles and associated expression of genes in their biosynthetic pathways. Four key anthocyanins were identified: cyanidin, cyanidin-3-O-rutinoside, delphinidin-3-glucoside, and delphinidin-3-rutinoside. Weighted gene co-expression network analysis (WGCNA) correlated the abundance of these four anthocyanins with transcriptomic data, to suggest three regulatory modules. Nine transcription factors families in these modules were identified and some of them were validated using qRT-PCR. Y2H assay isolated some transcription factors interacted with TTG1 (WD40 protein), including MYB3/39/44/306 and bHLH13/34/110, illustrating the possibility of forming MBW complexes. Our study provides a comprehensive characterization of anthocyanin composition. These findings laid a theoretical foundation for future research on the regulatory mechanisms of pigment accumulation and the breeding of <i>Hippeastrum</i> cultivars with novel petal colors. |
| format | Article |
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| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-b93f2c571cf34e44a12e6dccc604cb692025-08-20T03:35:36ZengMDPI AGAgronomy2073-43952025-07-01157172210.3390/agronomy15071722Molecular Elucidation of Anthocyanin Accumulation Mechanisms in <i>Hippeastrum hybridum</i> CultivarsPengyu Guo0Chuanji Xing1Jiacheng Ye2Jing Xue3Luis A. J. Mur4Bao Di5Zongli Hu6Guoping Chen7Xiuhai Zhang8Xuqing Chen9Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaThe Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Ceredigion SY23 3DA, UKCollege of Horticulture, Hebei Agriculture University, Baoding 071000, ChinaBioengineering College, Chongqing University, Chongqing 400044, ChinaBioengineering College, Chongqing University, Chongqing 400044, ChinaInstitute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China<i>Hippeastrum</i>, a perennial herbaceous plant belonging to the <i>Amaryllidaceae</i> family, is widely cultivated for its large, vibrant flowers with diverse petal colors, which have significant ornamental and economic value. However, the mechanisms underlying anthocyanin accumulation in <i>Hippeastrum</i> petals remain poorly understood. To fully explore the involved regulation mechanism was significant for the breeding of <i>Hippeastrum</i> and other <i>Amaryllidaceae</i> family plants. In this study, we selected six <i>Hippeastrum</i> cultivars with distinctly different petal colors. We used metabolomic profiling and high-throughput transcriptomic sequencing to assess varied anthocyanin profiles and associated expression of genes in their biosynthetic pathways. Four key anthocyanins were identified: cyanidin, cyanidin-3-O-rutinoside, delphinidin-3-glucoside, and delphinidin-3-rutinoside. Weighted gene co-expression network analysis (WGCNA) correlated the abundance of these four anthocyanins with transcriptomic data, to suggest three regulatory modules. Nine transcription factors families in these modules were identified and some of them were validated using qRT-PCR. Y2H assay isolated some transcription factors interacted with TTG1 (WD40 protein), including MYB3/39/44/306 and bHLH13/34/110, illustrating the possibility of forming MBW complexes. Our study provides a comprehensive characterization of anthocyanin composition. These findings laid a theoretical foundation for future research on the regulatory mechanisms of pigment accumulation and the breeding of <i>Hippeastrum</i> cultivars with novel petal colors.https://www.mdpi.com/2073-4395/15/7/1722<i>Hippeastrum</i>petalsanthocyaninstranscriptomicsmetabolomicsWGCNA |
| spellingShingle | Pengyu Guo Chuanji Xing Jiacheng Ye Jing Xue Luis A. J. Mur Bao Di Zongli Hu Guoping Chen Xiuhai Zhang Xuqing Chen Molecular Elucidation of Anthocyanin Accumulation Mechanisms in <i>Hippeastrum hybridum</i> Cultivars Agronomy <i>Hippeastrum</i> petals anthocyanins transcriptomics metabolomics WGCNA |
| title | Molecular Elucidation of Anthocyanin Accumulation Mechanisms in <i>Hippeastrum hybridum</i> Cultivars |
| title_full | Molecular Elucidation of Anthocyanin Accumulation Mechanisms in <i>Hippeastrum hybridum</i> Cultivars |
| title_fullStr | Molecular Elucidation of Anthocyanin Accumulation Mechanisms in <i>Hippeastrum hybridum</i> Cultivars |
| title_full_unstemmed | Molecular Elucidation of Anthocyanin Accumulation Mechanisms in <i>Hippeastrum hybridum</i> Cultivars |
| title_short | Molecular Elucidation of Anthocyanin Accumulation Mechanisms in <i>Hippeastrum hybridum</i> Cultivars |
| title_sort | molecular elucidation of anthocyanin accumulation mechanisms in i hippeastrum hybridum i cultivars |
| topic | <i>Hippeastrum</i> petals anthocyanins transcriptomics metabolomics WGCNA |
| url | https://www.mdpi.com/2073-4395/15/7/1722 |
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