Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-system
An optical fiber biosensor has been developed for detection of Hg(II) ion based on inhibition of urease immobilized onto alginate–chitosan membrane, coupled with bromothymol blue (BTB) in the flow-system. In order to get good performance of the biosensor toward Hg(II) ion detection, the experimenta...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Kuwait Journal of Science |
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| Online Access: | https://journalskuwait.org/kjs/index.php/KJS/article/view/9400 |
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| author | Dhony Hermanto Mudasir Dwi Siswanta Bambang Kuswandi Nurul Ismillayli |
| author_facet | Dhony Hermanto Mudasir Dwi Siswanta Bambang Kuswandi Nurul Ismillayli |
| author_sort | Dhony Hermanto |
| collection | DOAJ |
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An optical fiber biosensor has been developed for detection of Hg(II) ion based on inhibition of urease immobilized onto alginate–chitosan membrane, coupled with bromothymol blue (BTB) in the flow-system. In order to get good performance of the biosensor toward Hg(II) ion detection, the experimental parameters of the biosensor were optimized. Here, the biosensor showed maximum wavelength at 580 nm, with the optimum pH at 6. The calibration curve had a dynamic working range at 10 to 500 µg/L of Hg(II) ion with a detection limit of 12.1 µg/L (equal to 10% inhibition) and highly reproducible (RSD= 0.86%). The regeneration of the biosensor has been performed by treating with 1 mM ethylenediaminetetraacetic acid (EDTA) solution where up to 5 times cycles have been achieved with the inhibition decrease to 9.94% from the original biosensor response. Applying the biosensors to real samples showed the conformity of results with the reference method, cold vapor-atomic absorption spectrometry (CV-AAS).
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| format | Article |
| id | doaj-art-b91c40d031fb40ffadb13a1992ba39c7 |
| institution | Kabale University |
| issn | 2307-4108 2307-4116 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Kuwait Journal of Science |
| spelling | doaj-art-b91c40d031fb40ffadb13a1992ba39c72025-08-20T03:26:48ZengElsevierKuwait Journal of Science2307-41082307-41162021-12-0149110.48129/kjs.v49i1.9400Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-systemDhony Hermanto0Mudasir1Dwi Siswanta2Bambang Kuswandi3Nurul Ismillayli4Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of MataramDept. of Chemistry, Faculty of Mathematics and Natural Sciences, University of Gadjah Mada Jl. Sekip Utara BLS 21, Bulaksumur–Sleman, Yogyakarta 55281, IndonesiaDept. of Chemistry, Faculty of Mathematics and Natural Sciences, University of Gadjah Mada Jl. Sekip Utara BLS 21, Bulaksumur–Sleman, Yogyakarta 55281, IndonesiaChemo and Biosensor Group, Faculty of Pharmacy, University of Jember Jl. Kalimantan I/2 Tegalboto Sumbersari, Jember East Java 68121, IndonesiaDept. of Chemistry, Faculty of Mathematics and Natural Sciences, University of Mataram Jl. Majapahit 62, Mataram West Nusa Tenggara, Indonesia 83125 An optical fiber biosensor has been developed for detection of Hg(II) ion based on inhibition of urease immobilized onto alginate–chitosan membrane, coupled with bromothymol blue (BTB) in the flow-system. In order to get good performance of the biosensor toward Hg(II) ion detection, the experimental parameters of the biosensor were optimized. Here, the biosensor showed maximum wavelength at 580 nm, with the optimum pH at 6. The calibration curve had a dynamic working range at 10 to 500 µg/L of Hg(II) ion with a detection limit of 12.1 µg/L (equal to 10% inhibition) and highly reproducible (RSD= 0.86%). The regeneration of the biosensor has been performed by treating with 1 mM ethylenediaminetetraacetic acid (EDTA) solution where up to 5 times cycles have been achieved with the inhibition decrease to 9.94% from the original biosensor response. Applying the biosensors to real samples showed the conformity of results with the reference method, cold vapor-atomic absorption spectrometry (CV-AAS). https://journalskuwait.org/kjs/index.php/KJS/article/view/9400alginate–chitosan membraneBTBHg(II) ionoptical fiber biosensorurease |
| spellingShingle | Dhony Hermanto Mudasir Dwi Siswanta Bambang Kuswandi Nurul Ismillayli Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-system Kuwait Journal of Science alginate–chitosan membrane BTB Hg(II) ion optical fiber biosensor urease |
| title | Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-system |
| title_full | Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-system |
| title_fullStr | Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-system |
| title_full_unstemmed | Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-system |
| title_short | Optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate-chitosan membrane in the flow-system |
| title_sort | optical fiber mercury biosensor based on immobilized urease and bromothymol blue onto the alginate chitosan membrane in the flow system |
| topic | alginate–chitosan membrane BTB Hg(II) ion optical fiber biosensor urease |
| url | https://journalskuwait.org/kjs/index.php/KJS/article/view/9400 |
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