Expression of Salivary MMP-3 in Periodontitis Patients
Background: Periodontitis is a chronic inflammatory disease characterized by the destruction of supporting oral tissues. The condition involves unique host responses within the oral environment including the production of selected matrix metalloproteinases (MMPs) and certain inflammatory mediators...
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| Format: | Article |
| Language: | English |
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ziauddin University
2025-04-01
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| Series: | Pakistan Journal of Medicine and Dentistry |
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| Online Access: | https://ojs.zu.edu.pk/pjmd/article/view/3564 |
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| _version_ | 1850199580851830784 |
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| author | Syeda Hura Anwar Saima Sameer Quraeshi Rehan Imad Fizza Saher |
| author_facet | Syeda Hura Anwar Saima Sameer Quraeshi Rehan Imad Fizza Saher |
| author_sort | Syeda Hura Anwar |
| collection | DOAJ |
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Background: Periodontitis is a chronic inflammatory disease characterized by the destruction of supporting oral tissues. The condition involves unique host responses within the oral environment including the production of selected matrix metalloproteinases (MMPs) and certain inflammatory mediators that take part in tissue remodeling. The study aimed to evaluate MMP-3 expression levels in saliva samples collected from healthy and diseased subjects.
Methods: 82 study participants were enrolled in this case-control study through convenience sampling technique from the Periodontology OPD of the Ziauddin College of Dentistry, Karachi, Pakistan from January 2023 to 2024. Participants were divided into cases and controls depending on their clinical periodontal status. Saliva samples were collected from all participants using the Passive Drool Method. Analysis of collected samples was performed through ELISA Assay Method to determine quantitative expression levels of MMP-3. SPSS software version 24 was used to run T-test and Mann-Whitney Test for two group comparisons. Correlation analysis of MMP-3 protein expression levels with periodontal parameters was performed by Pearson’s Correlation. ROC curve was produced to determine the best cut-off value using Youden’s Index with 0.05 level of significance.
Results: Salivary expression levels of MMP-3 was significantly higher in periodontitis patients when compared to controls. MMP-3 expression was positively correlated with periodontal parameters including periodontal pocket depth, clinical attachment loss, bleeding on probing, and plaque score.
Conclusion: MMP-3 was strongly associated with periodontitis and its expression levels could be implicated to determine disease progression.
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| format | Article |
| id | doaj-art-b91b7fc57dad417c868a426c4749f0b6 |
| institution | OA Journals |
| issn | 2313-7371 2308-2593 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | ziauddin University |
| record_format | Article |
| series | Pakistan Journal of Medicine and Dentistry |
| spelling | doaj-art-b91b7fc57dad417c868a426c4749f0b62025-08-20T02:12:34Zengziauddin UniversityPakistan Journal of Medicine and Dentistry2313-73712308-25932025-04-0114210.36283/ziun-pjmd14-2/012Expression of Salivary MMP-3 in Periodontitis PatientsSyeda Hura Anwar0https://orcid.org/0009-0001-1978-7451Saima Sameer Quraeshi1https://orcid.org/0000-0002-5259-597XRehan Imad2https://orcid.org/0000-0003-0560-0171Fizza Saher3https://orcid.org/0000-0003-3953-8222Ziauddin College of Dentistry, Ziauddin University, Karachi, Pakistan.Ziauddin College of Dentistry, Ziauddin University, Karachi, Pakistan.Ziauddin University, Karachi, Pakistan.Ziauddin College of Dentistry, Ziauddin University, Karachi, Pakistan Background: Periodontitis is a chronic inflammatory disease characterized by the destruction of supporting oral tissues. The condition involves unique host responses within the oral environment including the production of selected matrix metalloproteinases (MMPs) and certain inflammatory mediators that take part in tissue remodeling. The study aimed to evaluate MMP-3 expression levels in saliva samples collected from healthy and diseased subjects. Methods: 82 study participants were enrolled in this case-control study through convenience sampling technique from the Periodontology OPD of the Ziauddin College of Dentistry, Karachi, Pakistan from January 2023 to 2024. Participants were divided into cases and controls depending on their clinical periodontal status. Saliva samples were collected from all participants using the Passive Drool Method. Analysis of collected samples was performed through ELISA Assay Method to determine quantitative expression levels of MMP-3. SPSS software version 24 was used to run T-test and Mann-Whitney Test for two group comparisons. Correlation analysis of MMP-3 protein expression levels with periodontal parameters was performed by Pearson’s Correlation. ROC curve was produced to determine the best cut-off value using Youden’s Index with 0.05 level of significance. Results: Salivary expression levels of MMP-3 was significantly higher in periodontitis patients when compared to controls. MMP-3 expression was positively correlated with periodontal parameters including periodontal pocket depth, clinical attachment loss, bleeding on probing, and plaque score. Conclusion: MMP-3 was strongly associated with periodontitis and its expression levels could be implicated to determine disease progression. https://ojs.zu.edu.pk/pjmd/article/view/3564Matrix Metalloproteinase Periodontitis Extracellular Matrix Saliva |
| spellingShingle | Syeda Hura Anwar Saima Sameer Quraeshi Rehan Imad Fizza Saher Expression of Salivary MMP-3 in Periodontitis Patients Pakistan Journal of Medicine and Dentistry Matrix Metalloproteinase Periodontitis Extracellular Matrix Saliva |
| title | Expression of Salivary MMP-3 in Periodontitis Patients |
| title_full | Expression of Salivary MMP-3 in Periodontitis Patients |
| title_fullStr | Expression of Salivary MMP-3 in Periodontitis Patients |
| title_full_unstemmed | Expression of Salivary MMP-3 in Periodontitis Patients |
| title_short | Expression of Salivary MMP-3 in Periodontitis Patients |
| title_sort | expression of salivary mmp 3 in periodontitis patients |
| topic | Matrix Metalloproteinase Periodontitis Extracellular Matrix Saliva |
| url | https://ojs.zu.edu.pk/pjmd/article/view/3564 |
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