Determination of the optimal method for measuring malondialdehyde in human saliva
While few methodological studies have been published on the salivary measurement of malondialdehyde (MDA), none have precisely detailed the analytical method. This work presents the development of an analytical method for MDA measurement in microvolumes of saliva samples from healthy individuals, us...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016124005211 |
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| author | H. Brignot C. Rayot G. Buiret T. Thomas-Danguin G. Feron |
| author_facet | H. Brignot C. Rayot G. Buiret T. Thomas-Danguin G. Feron |
| author_sort | H. Brignot |
| collection | DOAJ |
| description | While few methodological studies have been published on the salivary measurement of malondialdehyde (MDA), none have precisely detailed the analytical method. This work presents the development of an analytical method for MDA measurement in microvolumes of saliva samples from healthy individuals, using thiobarbituric acid derivatization and fluorescence reading of the formed compound. This method was progressively designed to meet specific constraints such as the limited sample volume available, cost-effectiveness of each assay, time required for analysis and costs. After studying the impact of various parameters, quantitative validation of this method was conducted using the accuracy profile. The study demonstrated that the fluorometric analysis method for salivary MDA accurately quantifies the concentration of this molecule within a validity range from 0.031 μM to 2.3 μM. Additionally, the accuracy profile allowed estimation of measurement uncertainty for each concentration level. It was determined that the calibration method using raw data was most appropriate as it introduced the least uncertainty in the obtained results. |
| format | Article |
| id | doaj-art-b8fa9de70b26497283ddbcdb2d8d39e4 |
| institution | Kabale University |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj-art-b8fa9de70b26497283ddbcdb2d8d39e42025-08-20T03:32:03ZengElsevierMethodsX2215-01612025-06-011410307010.1016/j.mex.2024.103070Determination of the optimal method for measuring malondialdehyde in human salivaH. Brignot0C. Rayot1G. Buiret2T. Thomas-Danguin3G. Feron4Centre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université de Bourgogne, F-21000 Dijon, FranceCentre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université de Bourgogne, F-21000 Dijon, FranceCentre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université de Bourgogne, F-21000 Dijon, France; Centre Hospitalier de Valence, 179 Boulevard du Maréchal Juin, 26953 Valence, France; Corresponding author.Centre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université de Bourgogne, F-21000 Dijon, FranceCentre des Sciences du Goût et de l'Alimentation, CNRS, INRAE, Institut Agro, Université de Bourgogne, F-21000 Dijon, FranceWhile few methodological studies have been published on the salivary measurement of malondialdehyde (MDA), none have precisely detailed the analytical method. This work presents the development of an analytical method for MDA measurement in microvolumes of saliva samples from healthy individuals, using thiobarbituric acid derivatization and fluorescence reading of the formed compound. This method was progressively designed to meet specific constraints such as the limited sample volume available, cost-effectiveness of each assay, time required for analysis and costs. After studying the impact of various parameters, quantitative validation of this method was conducted using the accuracy profile. The study demonstrated that the fluorometric analysis method for salivary MDA accurately quantifies the concentration of this molecule within a validity range from 0.031 μM to 2.3 μM. Additionally, the accuracy profile allowed estimation of measurement uncertainty for each concentration level. It was determined that the calibration method using raw data was most appropriate as it introduced the least uncertainty in the obtained results.http://www.sciencedirect.com/science/article/pii/S2215016124005211Using TBA derivatization and fluorescence |
| spellingShingle | H. Brignot C. Rayot G. Buiret T. Thomas-Danguin G. Feron Determination of the optimal method for measuring malondialdehyde in human saliva MethodsX Using TBA derivatization and fluorescence |
| title | Determination of the optimal method for measuring malondialdehyde in human saliva |
| title_full | Determination of the optimal method for measuring malondialdehyde in human saliva |
| title_fullStr | Determination of the optimal method for measuring malondialdehyde in human saliva |
| title_full_unstemmed | Determination of the optimal method for measuring malondialdehyde in human saliva |
| title_short | Determination of the optimal method for measuring malondialdehyde in human saliva |
| title_sort | determination of the optimal method for measuring malondialdehyde in human saliva |
| topic | Using TBA derivatization and fluorescence |
| url | http://www.sciencedirect.com/science/article/pii/S2215016124005211 |
| work_keys_str_mv | AT hbrignot determinationoftheoptimalmethodformeasuringmalondialdehydeinhumansaliva AT crayot determinationoftheoptimalmethodformeasuringmalondialdehydeinhumansaliva AT gbuiret determinationoftheoptimalmethodformeasuringmalondialdehydeinhumansaliva AT tthomasdanguin determinationoftheoptimalmethodformeasuringmalondialdehydeinhumansaliva AT gferon determinationoftheoptimalmethodformeasuringmalondialdehydeinhumansaliva |