Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study
Aims. The prognostic role of the proteases uPA and PAI-1, as well as tumor budding, in colon cancer, has been investigated previously. Methods. We provide 6-year follow-up data and results of the validation set. The initial test set and validation set consisted of 55 colon cancers and 68 colorectal...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2017/6504960 |
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| author | Bruno Märkl Jochen Hardt Simon Franz Tina Schaller Gerhard Schenkirsch Bernadette Kriening Reinhard Hoffmann Stefan Rüth |
| author_facet | Bruno Märkl Jochen Hardt Simon Franz Tina Schaller Gerhard Schenkirsch Bernadette Kriening Reinhard Hoffmann Stefan Rüth |
| author_sort | Bruno Märkl |
| collection | DOAJ |
| description | Aims. The prognostic role of the proteases uPA and PAI-1, as well as tumor budding, in colon cancer, has been investigated previously. Methods. We provide 6-year follow-up data and results of the validation set. The initial test set and validation set consisted of 55 colon cancers and 68 colorectal cancers, respectively. Tissue samples were analyzed for uPA and PAI-1 using a commercially available Enzyme-Linked Immunosorbent Assay (ELISA). Tumor budding was analyzed on cytokeratin-stained slides. Survival analyses were performed using cut-offs that were determined previously. Results. uPA was not prognostic for outcome. PAI-1 showed a trend towards reduced cancer specific survival in PAI-1 high-grade cases (68 versus 83 months; P=0.091). The combination of high-grade PAI-1 and tumor budding was associated with significantly reduced cancer specific survival (60 versus 83 months; P=0.021). After pooling the data from both sets, multivariate analyses revealed that the factors pN-stage, V-stage, and a combination of tumor budding and PAI-1 were independently prognostic for the association with distant metastases. Conclusions. A synergistic adverse effect of PAI-1 and tumor budding in uni- and multivariable analyses was found. PAI-1 could serve as a target for anticancer therapy. |
| format | Article |
| id | doaj-art-b8da73f435324d969138351df4a01df1 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-b8da73f435324d969138351df4a01df12025-02-03T06:05:04ZengWileyGastroenterology Research and Practice1687-61211687-630X2017-01-01201710.1155/2017/65049606504960Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective StudyBruno Märkl0Jochen Hardt1Simon Franz2Tina Schaller3Gerhard Schenkirsch4Bernadette Kriening5Reinhard Hoffmann6Stefan Rüth7Institute of Pathology, Klinikum Augsburg, Augsburg, GermanyInstitute of Laboratory Medicine and Microbiology, Klinikum Augsburg, Augsburg, GermanyInstitute of Pathology, Klinikum Augsburg, Augsburg, GermanyInstitute of Pathology, Klinikum Augsburg, Augsburg, GermanyClinical and Population-Based Cancer Registry Augsburg, Augsburg, GermanyDepartment of Visceral Surgery, Klinikum Augsburg, Augsburg, GermanyInstitute of Laboratory Medicine and Microbiology, Klinikum Augsburg, Augsburg, GermanyDepartment of Visceral Surgery, Klinikum Augsburg, Augsburg, GermanyAims. The prognostic role of the proteases uPA and PAI-1, as well as tumor budding, in colon cancer, has been investigated previously. Methods. We provide 6-year follow-up data and results of the validation set. The initial test set and validation set consisted of 55 colon cancers and 68 colorectal cancers, respectively. Tissue samples were analyzed for uPA and PAI-1 using a commercially available Enzyme-Linked Immunosorbent Assay (ELISA). Tumor budding was analyzed on cytokeratin-stained slides. Survival analyses were performed using cut-offs that were determined previously. Results. uPA was not prognostic for outcome. PAI-1 showed a trend towards reduced cancer specific survival in PAI-1 high-grade cases (68 versus 83 months; P=0.091). The combination of high-grade PAI-1 and tumor budding was associated with significantly reduced cancer specific survival (60 versus 83 months; P=0.021). After pooling the data from both sets, multivariate analyses revealed that the factors pN-stage, V-stage, and a combination of tumor budding and PAI-1 were independently prognostic for the association with distant metastases. Conclusions. A synergistic adverse effect of PAI-1 and tumor budding in uni- and multivariable analyses was found. PAI-1 could serve as a target for anticancer therapy.http://dx.doi.org/10.1155/2017/6504960 |
| spellingShingle | Bruno Märkl Jochen Hardt Simon Franz Tina Schaller Gerhard Schenkirsch Bernadette Kriening Reinhard Hoffmann Stefan Rüth Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study Gastroenterology Research and Practice |
| title | Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study |
| title_full | Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study |
| title_fullStr | Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study |
| title_full_unstemmed | Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study |
| title_short | Tumor Budding, uPA, and PAI-1 in Colorectal Cancer: Update of a Prospective Study |
| title_sort | tumor budding upa and pai 1 in colorectal cancer update of a prospective study |
| url | http://dx.doi.org/10.1155/2017/6504960 |
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