Osteogenic potential of calcium silicate-doped iron oxide nanoparticles versus calcium silicate for reconstruction of critical-sized mandibular defects: An experimental study in dog model
Objective: To evaluate bioactivity and osteogenic potential of calcium silicate (CS)-doped iron oxide (Fe2O3) nanoparticles versus pure CS in the reconstruction of induced critical-sized mandibular defects. Design: CS-doped Fe2O3 was prepared; morphological and microstructure identification of nanop...
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| Language: | English |
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Springer
2022-09-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905222000876 |
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| author | Said K. Taha Mohamed A. Abdel Hamid Esmat M.A. Hamzawy Sayed H. Kenawy Gehan T. El-Bassyouni Elham A. Hassan Heba E. Tarek |
| author_facet | Said K. Taha Mohamed A. Abdel Hamid Esmat M.A. Hamzawy Sayed H. Kenawy Gehan T. El-Bassyouni Elham A. Hassan Heba E. Tarek |
| author_sort | Said K. Taha |
| collection | DOAJ |
| description | Objective: To evaluate bioactivity and osteogenic potential of calcium silicate (CS)-doped iron oxide (Fe2O3) nanoparticles versus pure CS in the reconstruction of induced critical-sized mandibular defects. Design: CS-doped Fe2O3 was prepared; morphological and microstructure identification of nanoparticles were made. An in vivo randomised design was developed on 24 adult male dogs where four critical-sized mandibular defects were created in each dog. Bone defects were allocated into control, CS, CS-3% Fe2O3 and CS-10% Fe2O3 group. Dogs were euthanized at 1 and 3 months (12 dog/time) for histopathologic and histomorphometric evaluation. Results: At three months, bone formation and maturation were evident where mean ± SD percent of mature bone was 2.66 ± 1.8, 9.9 ± 2.5, 22.9 ± 4.9, and 38.6 ± 8.1 in control, CS, CS-3% Fe2O3, and CS-10% Fe2O3 groups respectively. A high significant (P < 0.001) increase in area percent of mature bone was recorded in CS, CS-3% Fe2O3, and CS- 10% Fe2O3 groups compared to control group (73%, 88% and 93.3% respectively). Significant increase (P < 0.001) in area of mature bone was recorded in CS-3% Fe2O3 and CS-10% Fe2O3 groups compared to CS group. A significant increase (P < 0.001) in area of mature bone formation was detected in CS-10% Fe2O3 group compared to other groups. Conclusion: CS-doped Fe2O3 has good osteoconductive, biocompatible properties with promoted bone regeneration. Fe2O3 has synergistic effect in combination with CS to promote bone formation. Increasing concentration of Fe2O3 nanoparticles resulted in improved osteogenesis and maturation. Results suggests that the novel CS-Fe2O3 alloplasts could be used for reconstruction of critical-sized bone defects. |
| format | Article |
| id | doaj-art-b8d3dfeca63d4b44956404c37e3be4fd |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Springer |
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| series | Saudi Dental Journal |
| spelling | doaj-art-b8d3dfeca63d4b44956404c37e3be4fd2025-08-20T01:55:58ZengSpringerSaudi Dental Journal1013-90522022-09-0134648549310.1016/j.sdentj.2022.06.008Osteogenic potential of calcium silicate-doped iron oxide nanoparticles versus calcium silicate for reconstruction of critical-sized mandibular defects: An experimental study in dog modelSaid K. Taha0Mohamed A. Abdel Hamid1Esmat M.A. Hamzawy2Sayed H. Kenawy3Gehan T. El-Bassyouni4Elham A. Hassan5Heba E. Tarek6Surgery and Oral Medicine Department, Oral and Dental Research Institute, National Research Centre, 33 El Buhouth St, Dokki, Giza 12622, EgyptDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptGlass Research Department, Advanced Materials Technology and Mineral Resources Research Institute, National Research Centre, 33 El Buhouth St, Dokki, Giza, 12622, EgyptRefractories, Ceramics and Building Materials Department, Advanced Materials Technology and Mineral Resources Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Giza 12622, EgyptRefractories, Ceramics and Building Materials Department, Advanced Materials Technology and Mineral Resources Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Giza 12622, EgyptDepartment of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt; Corresponding author.Basic Dental Science Department, Oral and Dental Research Institute, National Research Centre, 33 El Buhouth St, Dokki, Giza 12622, EgyptObjective: To evaluate bioactivity and osteogenic potential of calcium silicate (CS)-doped iron oxide (Fe2O3) nanoparticles versus pure CS in the reconstruction of induced critical-sized mandibular defects. Design: CS-doped Fe2O3 was prepared; morphological and microstructure identification of nanoparticles were made. An in vivo randomised design was developed on 24 adult male dogs where four critical-sized mandibular defects were created in each dog. Bone defects were allocated into control, CS, CS-3% Fe2O3 and CS-10% Fe2O3 group. Dogs were euthanized at 1 and 3 months (12 dog/time) for histopathologic and histomorphometric evaluation. Results: At three months, bone formation and maturation were evident where mean ± SD percent of mature bone was 2.66 ± 1.8, 9.9 ± 2.5, 22.9 ± 4.9, and 38.6 ± 8.1 in control, CS, CS-3% Fe2O3, and CS-10% Fe2O3 groups respectively. A high significant (P < 0.001) increase in area percent of mature bone was recorded in CS, CS-3% Fe2O3, and CS- 10% Fe2O3 groups compared to control group (73%, 88% and 93.3% respectively). Significant increase (P < 0.001) in area of mature bone was recorded in CS-3% Fe2O3 and CS-10% Fe2O3 groups compared to CS group. A significant increase (P < 0.001) in area of mature bone formation was detected in CS-10% Fe2O3 group compared to other groups. Conclusion: CS-doped Fe2O3 has good osteoconductive, biocompatible properties with promoted bone regeneration. Fe2O3 has synergistic effect in combination with CS to promote bone formation. Increasing concentration of Fe2O3 nanoparticles resulted in improved osteogenesis and maturation. Results suggests that the novel CS-Fe2O3 alloplasts could be used for reconstruction of critical-sized bone defects.http://www.sciencedirect.com/science/article/pii/S1013905222000876Calcium silicateIron oxide nanoparticlesMandibleDefectBone regenerationDog |
| spellingShingle | Said K. Taha Mohamed A. Abdel Hamid Esmat M.A. Hamzawy Sayed H. Kenawy Gehan T. El-Bassyouni Elham A. Hassan Heba E. Tarek Osteogenic potential of calcium silicate-doped iron oxide nanoparticles versus calcium silicate for reconstruction of critical-sized mandibular defects: An experimental study in dog model Saudi Dental Journal Calcium silicate Iron oxide nanoparticles Mandible Defect Bone regeneration Dog |
| title | Osteogenic potential of calcium silicate-doped iron oxide nanoparticles versus calcium silicate for reconstruction of critical-sized mandibular defects: An experimental study in dog model |
| title_full | Osteogenic potential of calcium silicate-doped iron oxide nanoparticles versus calcium silicate for reconstruction of critical-sized mandibular defects: An experimental study in dog model |
| title_fullStr | Osteogenic potential of calcium silicate-doped iron oxide nanoparticles versus calcium silicate for reconstruction of critical-sized mandibular defects: An experimental study in dog model |
| title_full_unstemmed | Osteogenic potential of calcium silicate-doped iron oxide nanoparticles versus calcium silicate for reconstruction of critical-sized mandibular defects: An experimental study in dog model |
| title_short | Osteogenic potential of calcium silicate-doped iron oxide nanoparticles versus calcium silicate for reconstruction of critical-sized mandibular defects: An experimental study in dog model |
| title_sort | osteogenic potential of calcium silicate doped iron oxide nanoparticles versus calcium silicate for reconstruction of critical sized mandibular defects an experimental study in dog model |
| topic | Calcium silicate Iron oxide nanoparticles Mandible Defect Bone regeneration Dog |
| url | http://www.sciencedirect.com/science/article/pii/S1013905222000876 |
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