Genetic Polymorphisms of the Vitamin D Receptor ApaI Gene and Physiological Parameters Diversity of Polycystic Ovary Syndrome in Iraqi Women

Polycystic ovary syndrome (PCOS) is a prevalent hormonal disorder among women of reproductive age, requiring diverse management strategies. This study, conducted at Kamal Al-Samarrai Hospital Fertility Center/Baghdad from January to March 2023, included 80 Iraqi women aged 18-45, comprising 40 with...

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Main Authors: Safa F. Ali, Shiva Khezri, Ilham A. Khalaf, Dalia J. Mahmood
Format: Article
Language:English
Published: Corporation of Research and Industrial Development 2024-06-01
Series:Iraqi Journal of Industrial Research
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Online Access:http://ijoir.gov.iq/ijoir/index.php/jou/article/view/418
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author Safa F. Ali
Shiva Khezri
Ilham A. Khalaf
Dalia J. Mahmood
author_facet Safa F. Ali
Shiva Khezri
Ilham A. Khalaf
Dalia J. Mahmood
author_sort Safa F. Ali
collection DOAJ
description Polycystic ovary syndrome (PCOS) is a prevalent hormonal disorder among women of reproductive age, requiring diverse management strategies. This study, conducted at Kamal Al-Samarrai Hospital Fertility Center/Baghdad from January to March 2023, included 80 Iraqi women aged 18-45, comprising 40 with PCOS and 40 healthy controls. Hormonal markers (FSH, LH, T, PRL, Ca2+, and Vitamin D3) were systematically assessed, along with Apa1 gene polymorphisms in whole blood genomic DNA using PCR-RFLP. PCOS patients showed significantly elevated LH, LH/FSH, T, and PRL levels (p < 0.01) and lower Vitamin D and calcium levels (p < 0.05) compared to controls. The scrutiny of VDR gene Apa1 polymorphism uncovered a notable prevalence of "Aa" and "aa" genotypes among PCOS patients, constituting 65.00% and 25.00%, respectively, in contrast to the control group's 17.50% and 0.00% in controls. The "aa" allele, more frequent in PCOS, emerged as a potential risk factor for the condition. This study establishes a strong association between VDR gene (Apa1) polymorphism and PCOS, highlighting the significance of the "aa" allele. It emphasizes the homozygous variant genotype "aa" as a substantial risk factor for PCOS, providing insights into its genetic basis.
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spelling doaj-art-b8bdd4b5a66a413896b50cfada5bd76f2025-08-20T02:39:13ZengCorporation of Research and Industrial DevelopmentIraqi Journal of Industrial Research2788-712X2024-06-0111110.53523/ijoirVol11I1ID418418Genetic Polymorphisms of the Vitamin D Receptor ApaI Gene and Physiological Parameters Diversity of Polycystic Ovary Syndrome in Iraqi WomenSafa F. Ali0Shiva Khezri1Ilham A. Khalaf2Dalia J. Mahmood3Al-Razi Center for Research and Diagnostic Kit Production/ Corporation of Research and Industrial Development, IraqDepartment of Biology, Faculty of Science, Urmia University, IranAl-Razi Center for Research and Diagnostic Kit Production/ Corporation of Research and Industrial Development, IraqKamal Al-Samarrai Hospital, Iraq Polycystic ovary syndrome (PCOS) is a prevalent hormonal disorder among women of reproductive age, requiring diverse management strategies. This study, conducted at Kamal Al-Samarrai Hospital Fertility Center/Baghdad from January to March 2023, included 80 Iraqi women aged 18-45, comprising 40 with PCOS and 40 healthy controls. Hormonal markers (FSH, LH, T, PRL, Ca2+, and Vitamin D3) were systematically assessed, along with Apa1 gene polymorphisms in whole blood genomic DNA using PCR-RFLP. PCOS patients showed significantly elevated LH, LH/FSH, T, and PRL levels (p < 0.01) and lower Vitamin D and calcium levels (p < 0.05) compared to controls. The scrutiny of VDR gene Apa1 polymorphism uncovered a notable prevalence of "Aa" and "aa" genotypes among PCOS patients, constituting 65.00% and 25.00%, respectively, in contrast to the control group's 17.50% and 0.00% in controls. The "aa" allele, more frequent in PCOS, emerged as a potential risk factor for the condition. This study establishes a strong association between VDR gene (Apa1) polymorphism and PCOS, highlighting the significance of the "aa" allele. It emphasizes the homozygous variant genotype "aa" as a substantial risk factor for PCOS, providing insights into its genetic basis. http://ijoir.gov.iq/ijoir/index.php/jou/article/view/418Polycystic ovary syndrome (PCOS)(VDR) geneAap1 gene
spellingShingle Safa F. Ali
Shiva Khezri
Ilham A. Khalaf
Dalia J. Mahmood
Genetic Polymorphisms of the Vitamin D Receptor ApaI Gene and Physiological Parameters Diversity of Polycystic Ovary Syndrome in Iraqi Women
Iraqi Journal of Industrial Research
Polycystic ovary syndrome (PCOS)
(VDR) gene
Aap1 gene
title Genetic Polymorphisms of the Vitamin D Receptor ApaI Gene and Physiological Parameters Diversity of Polycystic Ovary Syndrome in Iraqi Women
title_full Genetic Polymorphisms of the Vitamin D Receptor ApaI Gene and Physiological Parameters Diversity of Polycystic Ovary Syndrome in Iraqi Women
title_fullStr Genetic Polymorphisms of the Vitamin D Receptor ApaI Gene and Physiological Parameters Diversity of Polycystic Ovary Syndrome in Iraqi Women
title_full_unstemmed Genetic Polymorphisms of the Vitamin D Receptor ApaI Gene and Physiological Parameters Diversity of Polycystic Ovary Syndrome in Iraqi Women
title_short Genetic Polymorphisms of the Vitamin D Receptor ApaI Gene and Physiological Parameters Diversity of Polycystic Ovary Syndrome in Iraqi Women
title_sort genetic polymorphisms of the vitamin d receptor apai gene and physiological parameters diversity of polycystic ovary syndrome in iraqi women
topic Polycystic ovary syndrome (PCOS)
(VDR) gene
Aap1 gene
url http://ijoir.gov.iq/ijoir/index.php/jou/article/view/418
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