The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion
In this study, ultrasonic-assisted enzymatic extraction (UAEE) method was used to obtain the small molecule dandelion polysaccharides. Based on single-factor experiments, the process of extraction the extraction conditions were further optimized by response surface method (RSM) combined with the Box...
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| Language: | English |
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Elsevier
2025-05-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417725001087 |
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| author | Shuang Ni Haolan Zhao Shaohua Yang Kai Cui |
| author_facet | Shuang Ni Haolan Zhao Shaohua Yang Kai Cui |
| author_sort | Shuang Ni |
| collection | DOAJ |
| description | In this study, ultrasonic-assisted enzymatic extraction (UAEE) method was used to obtain the small molecule dandelion polysaccharides. Based on single-factor experiments, the process of extraction the extraction conditions were further optimized by response surface method (RSM) combined with the Box-Behnken design (BBD). The results showed that in combination with enzymes, with a ratio of 1:32 (g/ml), an enzymolysis temperature 55 °C, an ultrasonic temperature 75℃ and the ultrasonic time 55 min. The maximum extraction yield of crude polysaccharides was 3.127 %. After purification by DEAE-cellulose chromatography and Sephadex G-75 gel filtration, a novel polysaccharide (DANP-Ⅱ) was extracted and the results of high performance liquid chromatography (HPLC) indicated its average molecular weight was 3.373 kDa. Gas chromatography (GC) analysis showed that DANP-Ⅱ was mainly contained by glucose and little of glucuronic acid, mannose, galactose, and arabinose. In vitro antioxidant experiments have demonstrated that DANP-Ⅱ can alleviate H2O2-induced cellular damage, reduce apoptosis rates, and exhibit robust antioxidant activity. Hence, DANP-Ⅱ can be utilized as a natural antioxidant to enhance protection against oxidative stress, providing theoretical guidance for the utilization of low-molecular-weight dandelion polysaccharides. |
| format | Article |
| id | doaj-art-b8bc3754793e4d9da83a0926d370744f |
| institution | OA Journals |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-b8bc3754793e4d9da83a0926d370744f2025-08-20T02:08:31ZengElsevierUltrasonics Sonochemistry1350-41772025-05-0111610732910.1016/j.ultsonch.2025.107329The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelionShuang Ni0Haolan Zhao1Shaohua Yang2Kai Cui3School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Corresponding authors.Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China; Corresponding authors.In this study, ultrasonic-assisted enzymatic extraction (UAEE) method was used to obtain the small molecule dandelion polysaccharides. Based on single-factor experiments, the process of extraction the extraction conditions were further optimized by response surface method (RSM) combined with the Box-Behnken design (BBD). The results showed that in combination with enzymes, with a ratio of 1:32 (g/ml), an enzymolysis temperature 55 °C, an ultrasonic temperature 75℃ and the ultrasonic time 55 min. The maximum extraction yield of crude polysaccharides was 3.127 %. After purification by DEAE-cellulose chromatography and Sephadex G-75 gel filtration, a novel polysaccharide (DANP-Ⅱ) was extracted and the results of high performance liquid chromatography (HPLC) indicated its average molecular weight was 3.373 kDa. Gas chromatography (GC) analysis showed that DANP-Ⅱ was mainly contained by glucose and little of glucuronic acid, mannose, galactose, and arabinose. In vitro antioxidant experiments have demonstrated that DANP-Ⅱ can alleviate H2O2-induced cellular damage, reduce apoptosis rates, and exhibit robust antioxidant activity. Hence, DANP-Ⅱ can be utilized as a natural antioxidant to enhance protection against oxidative stress, providing theoretical guidance for the utilization of low-molecular-weight dandelion polysaccharides.http://www.sciencedirect.com/science/article/pii/S1350417725001087DandelionPolysaccharidesUAEEComponentAntioxidant activity |
| spellingShingle | Shuang Ni Haolan Zhao Shaohua Yang Kai Cui The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion Ultrasonics Sonochemistry Dandelion Polysaccharides UAEE Component Antioxidant activity |
| title | The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion |
| title_full | The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion |
| title_fullStr | The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion |
| title_full_unstemmed | The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion |
| title_short | The effect of ultrasonic-assisted enzymes extraction on antioxidant polysaccharide activity in dandelion |
| title_sort | effect of ultrasonic assisted enzymes extraction on antioxidant polysaccharide activity in dandelion |
| topic | Dandelion Polysaccharides UAEE Component Antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S1350417725001087 |
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