Analysis of glutathione and vitamin C effects
The alkaline single-cell gel electrophoresis (or Comet) assay was applied to evaluate the eventual DNA damage induced by the triphenolic metabolite of benzene, 1,2,4-benzenetriol (BT), in isolated human lymphocytes. Prior to BT treatment, ranging from 5 to 50 μM, a supplementation with glutathione (...
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2006.205 |
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| author | M. Pitarque A. Creus R. Marcos |
| author_facet | M. Pitarque A. Creus R. Marcos |
| author_sort | M. Pitarque |
| collection | DOAJ |
| description | The alkaline single-cell gel electrophoresis (or Comet) assay was applied to evaluate the eventual DNA damage induced by the triphenolic metabolite of benzene, 1,2,4-benzenetriol (BT), in isolated human lymphocytes. Prior to BT treatment, ranging from 5 to 50 μM, a supplementation with glutathione (GSH, 350 μg/ml) was carried out to assess whether GSH may have a modulating effect on the Comet response. The effect of a fixed dose of BT was also evaluated in the presence of the exogenous antioxidant vitamin C (40 and 200 μM). Additionally, we investigated whether the polymorphism of glutathione S-transferase T1 (GSTT1) gene may affect the individual level of BT-induced DNA damage in vitro. For all donors included in the present study, BT produced a significant dose-response relationship. No clear effect of GSH preincubation was seen on the BT-induced response. On the contrary, a significant reduction of DNA damage was observed in the presence of vitamin C (at least at 200 μM). Although our data suggest some individual differences according to the GSTT1 genotype in the outcome of the Comet assay, a large number of individuals should be studied in further investigations to obtain reliable conclusions. |
| format | Article |
| id | doaj-art-b8a208d843584e3c8ffd84972a4fd45a |
| institution | DOAJ |
| issn | 1537-744X |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-b8a208d843584e3c8ffd84972a4fd45a2025-08-20T03:22:50ZengWileyThe Scientific World Journal1537-744X2006-01-0161191120110.1100/tsw.2006.205Analysis of glutathione and vitamin C effectsM. Pitarque0A. Creus1R. Marcos2Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainGrup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainGrup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainThe alkaline single-cell gel electrophoresis (or Comet) assay was applied to evaluate the eventual DNA damage induced by the triphenolic metabolite of benzene, 1,2,4-benzenetriol (BT), in isolated human lymphocytes. Prior to BT treatment, ranging from 5 to 50 μM, a supplementation with glutathione (GSH, 350 μg/ml) was carried out to assess whether GSH may have a modulating effect on the Comet response. The effect of a fixed dose of BT was also evaluated in the presence of the exogenous antioxidant vitamin C (40 and 200 μM). Additionally, we investigated whether the polymorphism of glutathione S-transferase T1 (GSTT1) gene may affect the individual level of BT-induced DNA damage in vitro. For all donors included in the present study, BT produced a significant dose-response relationship. No clear effect of GSH preincubation was seen on the BT-induced response. On the contrary, a significant reduction of DNA damage was observed in the presence of vitamin C (at least at 200 μM). Although our data suggest some individual differences according to the GSTT1 genotype in the outcome of the Comet assay, a large number of individuals should be studied in further investigations to obtain reliable conclusions.http://dx.doi.org/10.1100/tsw.2006.205 |
| spellingShingle | M. Pitarque A. Creus R. Marcos Analysis of glutathione and vitamin C effects The Scientific World Journal |
| title | Analysis of glutathione and vitamin C effects |
| title_full | Analysis of glutathione and vitamin C effects |
| title_fullStr | Analysis of glutathione and vitamin C effects |
| title_full_unstemmed | Analysis of glutathione and vitamin C effects |
| title_short | Analysis of glutathione and vitamin C effects |
| title_sort | analysis of glutathione and vitamin c effects |
| url | http://dx.doi.org/10.1100/tsw.2006.205 |
| work_keys_str_mv | AT mpitarque analysisofglutathioneandvitaminceffects AT acreus analysisofglutathioneandvitaminceffects AT rmarcos analysisofglutathioneandvitaminceffects |