A bacteriocin-based coating strategy to prevent vancomycin-resistant Enterococcus faecium biofilm formation on materials of interest for indwelling medical devices
The ever-increasing use of exogenous materials as indwelling medical devices in modern medicine offers to pathogens new ways to gain access to human body and begin, in some cases, life threatening infections. Biofouling of such materials with bacteria or fungi is a major concern during surgeries, si...
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Elsevier
2024-12-01
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| Series: | Biofilm |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207524000364 |
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| author | Christian Kranjec Jills Puthiaparambil Mathew Kirill Ovchinnikov Idowu Fadayomi Ying Yang Morten Kjos Wen-Wu Li |
| author_facet | Christian Kranjec Jills Puthiaparambil Mathew Kirill Ovchinnikov Idowu Fadayomi Ying Yang Morten Kjos Wen-Wu Li |
| author_sort | Christian Kranjec |
| collection | DOAJ |
| description | The ever-increasing use of exogenous materials as indwelling medical devices in modern medicine offers to pathogens new ways to gain access to human body and begin, in some cases, life threatening infections. Biofouling of such materials with bacteria or fungi is a major concern during surgeries, since this is often associated with biofilm formation and difficult to treat, recalcitrant infections. Intense research efforts have therefore developed several strategies to shield the medical devices' surface from colonization by pathogenic microorganisms. Here, we used dopamine as a coupling agent to coat four different materials of medical interest (plastic polyetheretherketone (PEEK), stainless steel, titanium and silicone catheter) with the bacteriocins, enterocin EJ97-short and the thiopeptide micrococcin P1. Water contact angle measurements and x-ray photoelectron spectroscopy were used to verify the effective coating of the materials. The effect of bacteriocins coated on these materials on the biofilm formation by a vancomycin resistant Enterococcus faecium (VRE) strain was studied by biofilm-oriented antimicrobial test (BOAT) and electron scanning microscopy. The in vitro biocompatibility of bacteriocin-modified biomaterials was tested on cultured human cells. The results demonstrated that the binding of the bacteriocins to the implant surfaces is achieved, and the two bacteriocins in combination could inhibit biofilm formation by E. faecium on all four materials. The modified implant showed no cytotoxicity to the human cells tested. Therefore, surface modification with the two bacteriocins may offer a novel and effective way to prevent biofilm formation on a wide range of implant materials. |
| format | Article |
| id | doaj-art-b8a1786745d34df6a62b002b293b592c |
| institution | DOAJ |
| issn | 2590-2075 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-b8a1786745d34df6a62b002b293b592c2025-08-20T02:50:27ZengElsevierBiofilm2590-20752024-12-01810021110.1016/j.bioflm.2024.100211A bacteriocin-based coating strategy to prevent vancomycin-resistant Enterococcus faecium biofilm formation on materials of interest for indwelling medical devicesChristian Kranjec0Jills Puthiaparambil Mathew1Kirill Ovchinnikov2Idowu Fadayomi3Ying Yang4Morten Kjos5Wen-Wu Li6Laboratory of Microbial Gene Technology, Faculty of Chemistry, Biotechnology and Food Science. Norwegian University of Life Sciences, 1430, Ås, Norway; Corresponding author.School of Pharmacy and Bioengineering, Guy Hilton Research Centre, Keele University, Stoke-on-Trent, ST4 7QB, UKLaboratory of Microbial Gene Technology, Faculty of Chemistry, Biotechnology and Food Science. Norwegian University of Life Sciences, 1430, Ås, NorwaySchool of Pharmacy and Bioengineering, Guy Hilton Research Centre, Keele University, Stoke-on-Trent, ST4 7QB, UKSchool of Pharmacy and Bioengineering, Guy Hilton Research Centre, Keele University, Stoke-on-Trent, ST4 7QB, UKLaboratory of Microbial Gene Technology, Faculty of Chemistry, Biotechnology and Food Science. Norwegian University of Life Sciences, 1430, Ås, Norway; Corresponding author.School of Pharmacy and Bioengineering, Guy Hilton Research Centre, Keele University, Stoke-on-Trent, ST4 7QB, UK; Corresponding author.The ever-increasing use of exogenous materials as indwelling medical devices in modern medicine offers to pathogens new ways to gain access to human body and begin, in some cases, life threatening infections. Biofouling of such materials with bacteria or fungi is a major concern during surgeries, since this is often associated with biofilm formation and difficult to treat, recalcitrant infections. Intense research efforts have therefore developed several strategies to shield the medical devices' surface from colonization by pathogenic microorganisms. Here, we used dopamine as a coupling agent to coat four different materials of medical interest (plastic polyetheretherketone (PEEK), stainless steel, titanium and silicone catheter) with the bacteriocins, enterocin EJ97-short and the thiopeptide micrococcin P1. Water contact angle measurements and x-ray photoelectron spectroscopy were used to verify the effective coating of the materials. The effect of bacteriocins coated on these materials on the biofilm formation by a vancomycin resistant Enterococcus faecium (VRE) strain was studied by biofilm-oriented antimicrobial test (BOAT) and electron scanning microscopy. The in vitro biocompatibility of bacteriocin-modified biomaterials was tested on cultured human cells. The results demonstrated that the binding of the bacteriocins to the implant surfaces is achieved, and the two bacteriocins in combination could inhibit biofilm formation by E. faecium on all four materials. The modified implant showed no cytotoxicity to the human cells tested. Therefore, surface modification with the two bacteriocins may offer a novel and effective way to prevent biofilm formation on a wide range of implant materials.http://www.sciencedirect.com/science/article/pii/S2590207524000364Antibiotic resistanceBacteriocinsBiofilmEnterococcus faeciumPEEKTitanium |
| spellingShingle | Christian Kranjec Jills Puthiaparambil Mathew Kirill Ovchinnikov Idowu Fadayomi Ying Yang Morten Kjos Wen-Wu Li A bacteriocin-based coating strategy to prevent vancomycin-resistant Enterococcus faecium biofilm formation on materials of interest for indwelling medical devices Biofilm Antibiotic resistance Bacteriocins Biofilm Enterococcus faecium PEEK Titanium |
| title | A bacteriocin-based coating strategy to prevent vancomycin-resistant Enterococcus faecium biofilm formation on materials of interest for indwelling medical devices |
| title_full | A bacteriocin-based coating strategy to prevent vancomycin-resistant Enterococcus faecium biofilm formation on materials of interest for indwelling medical devices |
| title_fullStr | A bacteriocin-based coating strategy to prevent vancomycin-resistant Enterococcus faecium biofilm formation on materials of interest for indwelling medical devices |
| title_full_unstemmed | A bacteriocin-based coating strategy to prevent vancomycin-resistant Enterococcus faecium biofilm formation on materials of interest for indwelling medical devices |
| title_short | A bacteriocin-based coating strategy to prevent vancomycin-resistant Enterococcus faecium biofilm formation on materials of interest for indwelling medical devices |
| title_sort | bacteriocin based coating strategy to prevent vancomycin resistant enterococcus faecium biofilm formation on materials of interest for indwelling medical devices |
| topic | Antibiotic resistance Bacteriocins Biofilm Enterococcus faecium PEEK Titanium |
| url | http://www.sciencedirect.com/science/article/pii/S2590207524000364 |
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