Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells
Abstract Lawsonia intracellularis, a Gram-negative obligate intracellular bacterium causing porcine proliferative enteropathy, possesses a type III secretion system (T3SS), yet only a handful of its substrates have been experimentally characterized. In this study, we identify that LI0758 can be secr...
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BMC
2025-02-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-025-01461-8 |
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| author | Yuanxiu Zhong Yiyun Duan Fenju Lai Jinhua Zhang Yimin Dai |
| author_facet | Yuanxiu Zhong Yiyun Duan Fenju Lai Jinhua Zhang Yimin Dai |
| author_sort | Yuanxiu Zhong |
| collection | DOAJ |
| description | Abstract Lawsonia intracellularis, a Gram-negative obligate intracellular bacterium causing porcine proliferative enteropathy, possesses a type III secretion system (T3SS), yet only a handful of its substrates have been experimentally characterized. In this study, we identify that LI0758 can be secreted by the Yersinia T3SS, which suppresses yeast growth and activates mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathway in mammalian cells. Bioinformatics analyses indicate that LI0758 is an ortholog of Rce1, a eukaryotic CAAX protein endoprotease, sharing a similar subcellular localization on the endoplasmic reticulum (ER). While displaying unique activity in the yeast a-factor reporter system, LI0758 restores Ras2 localization in Rce1Δ mutant strains, implying functional similarity. Our findings underscore LI0758’s pivotal role in activating MAPK pathways and suggest its potential to modulate the localization and function of host CAAX proteins. Further investigation holds promise for elucidating novel bacteria-host interaction mechanisms and fostering the development of innovative therapies against proliferative enteritis. |
| format | Article |
| id | doaj-art-b8a0ec70ed6d426cb2e347e86376db23 |
| institution | Kabale University |
| issn | 1297-9716 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-b8a0ec70ed6d426cb2e347e86376db232025-02-09T12:50:12ZengBMCVeterinary Research1297-97162025-02-0156111610.1186/s13567-025-01461-8Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cellsYuanxiu Zhong0Yiyun Duan1Fenju Lai2Jinhua Zhang3Yimin Dai4School of Biological Science and Engineering, Jiangxi Agricultural UniversitySchool of Biological Science and Engineering, Jiangxi Agricultural UniversitySchool of Biological Science and Engineering, Jiangxi Agricultural UniversitySchool of Animal Science and Technology, Jiangxi Agricultural UniversitySchool of Animal Science and Technology, Jiangxi Agricultural UniversityAbstract Lawsonia intracellularis, a Gram-negative obligate intracellular bacterium causing porcine proliferative enteropathy, possesses a type III secretion system (T3SS), yet only a handful of its substrates have been experimentally characterized. In this study, we identify that LI0758 can be secreted by the Yersinia T3SS, which suppresses yeast growth and activates mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathway in mammalian cells. Bioinformatics analyses indicate that LI0758 is an ortholog of Rce1, a eukaryotic CAAX protein endoprotease, sharing a similar subcellular localization on the endoplasmic reticulum (ER). While displaying unique activity in the yeast a-factor reporter system, LI0758 restores Ras2 localization in Rce1Δ mutant strains, implying functional similarity. Our findings underscore LI0758’s pivotal role in activating MAPK pathways and suggest its potential to modulate the localization and function of host CAAX proteins. Further investigation holds promise for elucidating novel bacteria-host interaction mechanisms and fostering the development of innovative therapies against proliferative enteritis.https://doi.org/10.1186/s13567-025-01461-8L. intracellularistype III secretion systemMAPKNF-κBprenylationRce1 |
| spellingShingle | Yuanxiu Zhong Yiyun Duan Fenju Lai Jinhua Zhang Yimin Dai Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells Veterinary Research L. intracellularis type III secretion system MAPK NF-κB prenylation Rce1 |
| title | Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells |
| title_full | Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells |
| title_fullStr | Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells |
| title_full_unstemmed | Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells |
| title_short | Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells |
| title_sort | lawsonia intracellularis t3ss effector li0758 an rce1 ortholog activates mapk and nf κb signaling in mammalian cells |
| topic | L. intracellularis type III secretion system MAPK NF-κB prenylation Rce1 |
| url | https://doi.org/10.1186/s13567-025-01461-8 |
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